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The action of a water-soluble carbodiimide on adenosine-5'-polyphosphates.

Nucleic Acids Research

Water, Solubility, Phosphoric Diester Hydrolases, Phosphodiesterase I, Hydrolysis, pharmacology, Ethyldimethylaminopropyl Carbodiimide, Carbodiimides, analogs & derivatives, CME-Carbodiimide, Alkaline Phosphatase, metabolism, Adenine Nucleotides

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      Abstract

      When aqueous solutions of adenosine-5'-mono-, di-, or triphosphates are treated with a water soluble carbodiimide the major product is the expected diadenosine-5'-5'-polyphosphate. The yields of these pyrophosphates are greatly increased in the presence of the Mg2+ ion. Adenosine-5'-tetraphosphate behaves differently. The major product is adenosine-5'-monophosphate. We believe that this hydrolysis occurs via a cyclic trimetaphosphate intermediate.

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      340750
      3033606
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