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      Acute and Long-Term Changes Observed in Imprints of Mouse Mesothelium Exposed to Glucose-Enriched, Lactated, Buffered Dialysis Solutions


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          Solutions for peritoneal dialysis (PD), the hyperosmolarity of which is obtained with glucose, have been shown to initiate and maintain a situation of continuous mesothelial cell injury associated with a process of regeneration which also takes place continuously. The present study was done using the in vivo and almost in situ technique of mesothelial cell imprints. Acute exposure to 4.25% glucose solution at pH 5.2 and 7.0 induced higher mitotic activity, defective cytokinesis, and reduced cell viability. Long-term exposure (15 and 30 days) to both 4.25% glucose solutions was associated with a reduced population density, increased surface area, and lower mesothelial cell viability, regardless of the pH. The use of 1.5% glucose fluid showed that this effect was dose related. After 30 days of recovery, mesothelial cells exposed to the high-glucose solution at both pH 5.2 and 7.0 appeared repopulated by small cells and showed evidence of defective cytokinesis. So far, it appears that the alterations observed after long-term exposure of the mesothelium to PD fluid are mainly caused by the high concentration of glucose per se. The additional effects of hyperosmolarity are still unclear, whereas the eventual role of low pH, at least in the experimental model used here, can be defined as less than marginal.

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          Author and article information

          S. Karger AG
          18 December 2008
          : 70
          : 4
          : 466-477
          Department of Nephrology, and Komach Laboratory for Experimental Nephrology, Central Emek Hospital, Afula, Israel
          188647 Nephron 1995;70:466–477
          © 1995 S. Karger AG, Basel

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          : 05 July 1994
          Page count
          Pages: 12
          Original Paper

          Cardiovascular Medicine,Nephrology
          Biocompatibility of peritoneal dialysis fluid,Mesothelial cell imprints,Peritoneal cytology,Peritoneal dialysis


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