Draft Genome Sequence of Leptospira interrogans Serovar Bataviae Strain LepIMR 22 Isolated from a Rodent in Johor, Malaysia

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Abstract

Leptospira interrogans serovar Bataviae was recently identified as one of the persistent Leptospira serovars in Malaysia. Here, we report the draft genome sequence of the L. interrogans serovar Bataviae strain LepIMR 22 isolated from kidney of a rodent in Johor, Malaysia.

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Leptospiral Pathogenomics

Jason S. Lehmann, Michael Matthias, Joseph Vinetz … (2014)

Leptospirosis, caused by pathogenic spirochetes belonging to the genus Leptospira, is a zoonosis with important impacts on human and animal health worldwide. Research on the mechanisms of Leptospira pathogenesis has been hindered due to slow growth of infectious strains, poor transformability, and a paucity of genetic tools. As a result of second generation sequencing technologies, there has been an acceleration of leptospiral genome sequencing efforts in the past decade, which has enabled a concomitant increase in functional genomics analyses of Leptospira pathogenesis. A pathogenomics approach, by coupling of pan-genomic analysis of multiple isolates with sequencing of experimentally attenuated highly pathogenic Leptospira, has resulted in the functional inference of virulence factors. The global Leptospira Genome Project supported by the U.S. National Institute of Allergy and Infectious Diseases to which key scientific contributions have been made from the international leptospirosis research community has provided a new roadmap for comprehensive studies of Leptospira and leptospirosis well into the future. This review describes functional genomics approaches to apply the data generated by the Leptospira Genome Project towards deepening our knowledge of virulence factors of Leptospira using the emerging discipline of pathogenomics.

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Determination of Leptospira borgpetersenii serovar Javanica and Leptospira interrogans serovar Bataviae as the persistent Leptospira serovars circulating in the urban rat populations in Peninsular Malaysia

Douadi Benacer, Siti Nursheena Mohd Zain, Shin Sim … (2016)

Background Leptospirosis is an emerging infectious disease of global significance, and is endemic in tropical countries, including Malaysia. Over the last decade, a dramatic increase of human cases was reported; however, information on the primary vector, the rat, and the Leptospira serovars circulating among the rat population is limited. Therefore, the present study was undertaken to isolate Leptospira and characterise the serovars circulating in the urban rat populations from selected main cities in Peninsular Malaysia. Methods Rat trappings were carried out between October 2011 to February 2014 in five urban cities which were chosen as study sites to represent different geographical locations in Peninsular Malaysia. Microscopic agglutination test (MAT) and PCR were carried out to identify the Leptospiral serogroup and determine the pathogenic status of the isolates, respectively while pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD)-PCR were used to characterize the isolates. Results Three rat species were identified from the three hundred and fifty seven rats captured with Rattus rattus, being the dominant rat species (285, 80 %) followed by Rattus norgevicus (53, 15 %) and Rattus exulans (19, 5 %). Only 39 samples (11.0 %) were positive by culture and further confirmed as pathogenic Leptospira by PCR. Significant associations were shown between host infection with locality, season, host-age and species. Based on MAT, two serogroups were identified in the population namely; L. borgpetersenii serogroup Javanica (n = 16) and L. interrogans serogroup Bataviae (n = 23). Pulsed-field gel electrophoresis (PFGE) distinguished the two serovars in the urban rat populations: L. borgpetersenii serovar Javanica (41 %), and L. interrogans serovar Bataviae (59 %). RAPD-PCR yielded 14 distinct patterns and was found to be more discriminative than PFGE. Conclusions This study confirms two Leptospira serovars circulating among the urban rats population in Peninsular Malaysia namely; L. borgpetersenii serovar Javanica and L. interrogans serovars Bataviae. Despite the low number of isolates obtained from the rat population, this study suggests that rodent control programs and disease surveillance may help to reduce the possible risk of disease transmission.

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Predominance of the ST143 and ST50 Leptospira clones in the urban rat populations of Peninsular Malaysia.

Douadi Benacer, Siti Nursheena Mohd Zain, Ahmed A Ahmed … (2016)

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Author and article information

Journal

Journal ID (nlm-ta): Genome Announc

Journal ID (iso-abbrev): Genome Announc

Journal ID (hwp): ga

Journal ID (pmc): ga

Journal ID (publisher-id): GA

Title: Genome Announcements

Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )

ISSN (Electronic): 2169-8287

Publication date (Electronic): 8 September 2016

Publication date Collection: Sep-Oct 2016

Volume: 4

Issue: 5

Electronic Location Identifier: e00956-16

Affiliations

[a ]Infectious Disease Research Centre, Bacteriology Unit, Institute for Medical Research, Kuala Lumpur, Malaysia

[b ]Specialised Diagnostics Centre, Molecular Diagnostics and Protein Unit, Institute for Medical Research, Kuala Lumpur, Malaysia

[c ]Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia

[d ]Primary Immunodeficiency Unit, Allergy and Immunology Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia

[e ]KIT Biomedical Research, Royal Tropical Institute (KIT), Amsterdam, The Netherlands

Author notes

Address correspondence to Mohd Khairul Nizam Mohd Khalid, mknizam@ 123456imr.gov.my .

Article

Publisher ID: genomeA00956-16

DOI: 10.1128/genomeA.00956-16

PMC ID: 5017229

PubMed ID: 27609924

SO-VID: 93c38c57-60cb-4fd8-9434-3cc863947944

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

History

Date received : 14 July 2016

Date accepted : 19 July 2016

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Figures: 0, Tables: 0, Equations: 0, References: 8, Pages: 2, Words: 975

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cover-date September/October 2016

ScienceOpen disciplines: Genetics

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ScienceOpen disciplines: Genetics

Draft Genome Sequence of Leptospira interrogans Serovar Bataviae Strain LepIMR 22 Isolated from a Rodent in Johor, Malaysia

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Leptospiral Pathogenomics

Determination of Leptospira borgpetersenii serovar Javanica and Leptospira interrogans serovar Bataviae as the persistent Leptospira serovars circulating in the urban rat populations in Peninsular Malaysia

Predominance of the ST143 and ST50 Leptospira clones in the urban rat populations of Peninsular Malaysia.

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