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      Lifeact: a versatile marker to visualize F-actin.

      Nature Methods
      Springer Science and Business Media LLC

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          Abstract

          Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.

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          Journal
          18536722
          2814344
          10.1038/nmeth.1220

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