15
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      The application of Ussing chambers for determining the impact of microbes and probiotics on intestinal ion transport.

      1 ,
      Canadian journal of physiology and pharmacology

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Host-microbe interactions have gained considerable attention in recent years with regards to their role in various organic disorders and diseases. In particular, research efforts have focused on the intestinal microbiota, where the largest and most diverse populations not only co-exist with the host, but also directly influence the state and function of the gastrointestinal (GI) tract. Moreover, both human and animal studies alike are now beginning to show a positive influence of probiotic bacteria on GI disorders associated with diarrhoea or constipation. Diarrheagenic GI diseases, such as those caused by Vibreo cholera or enterpathogenic Eschericia coli, have well-characterised interactions with the host that explain much of the observed symptoms, in particular severe diarrhoea. However, the mechanisms of action of nonpathogenic bacteria or probiotics on host physiology are less clearly understood. In the context of defining the mechanisms of action of probiotics in vitro, the Ussing chamber has proven to be a particularly useful tool. Here, we will present data from several studies that have defined molecular targets for microbes and putative probiotics in the regulation of intestinal secretory and absorptive function, and we will discuss these in the context of their application in pathogen- or inflammation-induced alterations in intestinal ion transport.

          Related collections

          Author and article information

          Journal
          Can. J. Physiol. Pharmacol.
          Canadian journal of physiology and pharmacology
          1205-7541
          0008-4212
          Sep 2013
          : 91
          : 9
          Affiliations
          [1 ] Department of Pharmacology and Therapeutics, Western Gateway Building, Western Road, University College Cork, Co. Cork, Ireland.
          Article
          10.1139/cjpp-2013-0027
          23984904
          951d60e1-5806-4433-9916-c71d92d4e7b7
          History

          Comments

          Comment on this article