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      • Record: found
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      Metabolic Analysis of the Rabbit Cornea by Proton Nuclear Magnetic Resonance Spectroscopy

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          Abstract

          Perchloric acid extracts of the rabbit cornea were analyzed by high-resolution <sup>1</sup>H NMR spectroscopy, including the two-dimensional shift-correlated (COSY) technique. Numerous metabolites including leucine/isoleucine, 3-hydroxybutyrate, valine, lactate, alanine, lysine, acetate, glutamate, creatine/ phosphocreatine, taurine, glycine, inositol, glucose, tyrosine, phenylalanine, histidine, adenosine and formate have been simultaneously detected and identified in the NMR spectra. Concentrations of some of these were also determined. Of particular importance are the detection and identification of hypotaurine in the corneal tissue. Hypotaurine is a precursor of the synthesis of taurine and may act as an antioxidant. Moreover, the presence of choline, a precursor for acetylcholine, was established. This novel metabolite profile of the cornea obtained by high-resolution l·H NMR spectroscopy may be of value in the study of pathologic and toxic disorders.

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          Author and article information

          Journal
          Journal ID (publisher-id): ORE
          Journal ID (nlm-ta): Ophthalmic Res
          Journal ID (doi): 10.1159/issn.0030-3747
          Title: Ophthalmic Research
          Publisher: S. Karger AG
          ISSN (Print): 0030-3747
          ISSN (Electronic): 1423-0259
          Publication date Subscription-year: 1996
          Publication date (Print): 1996
          Publication date (Electronic): 11 December 2009
          Volume: 28
          Issue: 5
          Pages: 319-329
          Affiliations
          aDepartment of Ophthalmology, Faculty of Medicine, and bDepartment of Biophysics, Norwegian Institute of Technology, University of Trondheim; cMR Center, Natural Science Section, SINTEF UNIMED, Trondheim, Norway
          Article
          Publisher ID: 267921 Other ID: Ophthalmic Res 1996;28:319–329
          DOI: 10.1159/000267921
          PubMed ID: 8979281
          SO-VID: 9553fbb3-b78a-4454-bfb2-47bdd0c93c8a
          Copyright © © 1996 S. Karger AG, Basel
          License:

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          Date received : 19 December 1995
          Date accepted : 11 June 1996
          Page count
          Pages: 11
          Categories
          Subject: Original Paper

          ScienceOpen disciplines: Vision sciences,Ophthalmology & Optometry,Pathology
          Keywords: High-resolution NMR spectroscopy,Cornea,Choline,Proton NMR spectroscopy,Hypotaurine,Amino acids,Metabolites
          Data availability:
          ScienceOpen disciplines: Vision sciences, Ophthalmology & Optometry, Pathology
          Keywords: High-resolution NMR spectroscopy, Cornea, Choline, Proton NMR spectroscopy, Hypotaurine, Amino acids, Metabolites

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