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      Metabolic Analysis of the Rabbit Cornea by Proton Nuclear Magnetic Resonance Spectroscopy

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          Perchloric acid extracts of the rabbit cornea were analyzed by high-resolution <sup>1</sup>H NMR spectroscopy, including the two-dimensional shift-correlated (COSY) technique. Numerous metabolites including leucine/isoleucine, 3-hydroxybutyrate, valine, lactate, alanine, lysine, acetate, glutamate, creatine/ phosphocreatine, taurine, glycine, inositol, glucose, tyrosine, phenylalanine, histidine, adenosine and formate have been simultaneously detected and identified in the NMR spectra. Concentrations of some of these were also determined. Of particular importance are the detection and identification of hypotaurine in the corneal tissue. Hypotaurine is a precursor of the synthesis of taurine and may act as an antioxidant. Moreover, the presence of choline, a precursor for acetylcholine, was established. This novel metabolite profile of the cornea obtained by high-resolution l·H NMR spectroscopy may be of value in the study of pathologic and toxic disorders.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          11 December 2009
          : 28
          : 5
          : 319-329
          aDepartment of Ophthalmology, Faculty of Medicine, and bDepartment of Biophysics, Norwegian Institute of Technology, University of Trondheim; cMR Center, Natural Science Section, SINTEF UNIMED, Trondheim, Norway
          267921 Ophthalmic Res 1996;28:319–329
          © 1996 S. Karger AG, Basel

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          Pages: 11
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