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      Metabolic Analysis of the Rabbit Cornea by Proton Nuclear Magnetic Resonance Spectroscopy

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          Abstract

          Perchloric acid extracts of the rabbit cornea were analyzed by high-resolution <sup>1</sup>H NMR spectroscopy, including the two-dimensional shift-correlated (COSY) technique. Numerous metabolites including leucine/isoleucine, 3-hydroxybutyrate, valine, lactate, alanine, lysine, acetate, glutamate, creatine/ phosphocreatine, taurine, glycine, inositol, glucose, tyrosine, phenylalanine, histidine, adenosine and formate have been simultaneously detected and identified in the NMR spectra. Concentrations of some of these were also determined. Of particular importance are the detection and identification of hypotaurine in the corneal tissue. Hypotaurine is a precursor of the synthesis of taurine and may act as an antioxidant. Moreover, the presence of choline, a precursor for acetylcholine, was established. This novel metabolite profile of the cornea obtained by high-resolution l·H NMR spectroscopy may be of value in the study of pathologic and toxic disorders.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1996
          1996
          11 December 2009
          : 28
          : 5
          : 319-329
          Affiliations
          aDepartment of Ophthalmology, Faculty of Medicine, and bDepartment of Biophysics, Norwegian Institute of Technology, University of Trondheim; cMR Center, Natural Science Section, SINTEF UNIMED, Trondheim, Norway
          Article
          267921 Ophthalmic Res 1996;28:319–329
          10.1159/000267921
          8979281
          © 1996 S. Karger AG, Basel

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          Page count
          Pages: 11
          Categories
          Original Paper

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