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      Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP.

      1 , ,
      Medical and veterinary entomology
      Wiley

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          Abstract

          For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.

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          Author and article information

          Journal
          Med Vet Entomol
          Medical and veterinary entomology
          Wiley
          0269-283X
          0269-283X
          Dec 2002
          : 16
          : 4
          Affiliations
          [1 ] London School of Hygiene and Tropical Medicine, London, UK.
          Article
          10.1046/j.1365-2915.2002.00393.x
          12510902
          957bab18-2eb4-4b36-a3c4-b4c0d31ab87c
          History

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