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Abstract
Two experiments were carried out. In the first, three groups of lambs were inoculated
subcutaneously with 3 X 10(6) schizonts of different passages (3, 30 and 63) of Theileria
hirci propagated in tissue culture. Severe reactions were observed in lambs inoculated
with organisms derived from the 3rd passage. In the second experiment, four groups
were inoculated with 5 X 10(5), 3 X 10(6), 1 X 10(7) and 5 X 10(8) schizonts of the
63rd passage. No clinical reactions or parasites were detected in lambs inoculated
with 5 X 10(5) schizonts. Mild reactions were observed in lambs inoculated with 3
X 10(6), 1 X 10(7), and 5 X 10(7) schizonts. Lambs inoculated with 3 X 10(6) schizonts
were resistant to challenge with a virulent strain. The indirect fluorescent antibody
(IFA) test was used to determine the antibody titre.