30
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Neurogenic phenotypes induced by RNA interference with bHLH genes of the Enhancer of split complex of Drosophila melanogaster.

      Genesis (New York, N.y. : 2000)
      Animals, Basic Helix-Loop-Helix Transcription Factors, DNA-Binding Proteins, genetics, Drosophila Proteins, antagonists & inhibitors, Drosophila melanogaster, Embryo, Nonmammalian, physiology, Enhancer Elements, Genetic, Gene Expression Regulation, Developmental, Nervous System, embryology, Phenotype, RNA Interference, Repressor Proteins, Transcription Factors

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The Enhancer of split gene complex [E(spl)-C] of Drosophila melanogaster harbors seven highly related genes encoding transcriptional regulators with a basic helix-loop-helix (bHLH) domain. They are activated by the Notch signaling pathway in order to inhibit proneural gene activity, for example, during neurogenesis in the developing embryo. The E(spl) proteins are at least partly redundant, despite some remarkable differences in their expression patterns. We attempted to address the degree of redundancy by means of RNA interference. We find a quantitative correlation between the degree of a neurogenic phenotype and the number of genes affected. Surprisingly, interference with m3 results in a high rate of mortality which cannot be reproduced by genetic mutation. Most likely, m3 dsRNA interferes with unrelated genes involved in other aspects of embryonic development. Copyright 2004 Wiley-Liss, Inc.

          Related collections

          Author and article information

          Comments

          Comment on this article