Experimental studies on the survival of infective stage larvae of horse strongyles and their ability to overwinter on pasture were carried out in central Ukraine (Poltavska oblast). Faecal pats (1.5 kg each) of naturally infected horses were placed on pasture, and samples of faeces and surrounding vegetation (10 g each) were collected each month, excluding the winter months, from November 2002 until April 2004. The number of infective third stage larvae was calculated in each sample and compared with that from the control faecal samples cultivated in the laboratory. In the control samples, the ratio of infective third stage larvae to the initial number of eggs was from 54.7% in June up to 84.2% in November. This ratio depended on the presence of nematophagous fungi growing in the faeces. On pasture, the development of larvae to the infective third stage took approximately 4 weeks in the warm season, from April until September. In October, a percentage of the eggs (25% to EPG value) did not hatch. No larval development was observed in faeces in November. A minute quantity of larvae, about 0.03% of their initial number, was observed to survive on pasture for the 12 months. Migration of infective larvae from the faeces to vegetation was not intensive, between 71% and 89% of larvae remained in the faeces 4 weeks after deposition of the faecal pats, the percentage related to soil humidity in each month. The proportion of larvae successfully surviving during winter appeared to be maximal in faecal pats deposited on pasture in September of the previous year (up to 42.0% of the initial number of larvae). Some larvae were observed surviving winter in soil beneath the faecal pats. The results of the study demonstrated that horse pastures in the central part of Ukraine are never free from the infective third stage larvae of strongyles.