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      Production of Hydrophobic Zein-Based Films Bioinspired by The Lotus Leaf Surface: Characterization and Bioactive Properties

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          Abstract

          Hydrophobic zein-based functional films incorporating licorice essential oil were successfully developed as new alternative materials for food packaging. The lotus-leaf negative template was obtained using polydimethylsiloxane (PDMS). The complex surface patterns of the lotus leaves were transferred onto the surface of the zein-based films with high fidelity (positive replica), which validates the proposed proof-of-concept. The films were prepared by casting method and fully characterized by Scanning Electron Microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC). The grammage, thickness, contact angle, mechanical, optical and barrier properties of the films were measured, together with the evaluation of their biodegradability, antioxidant and antibacterial activities against common foodborne pathogens ( Enterococcus faecalis and Listeria monocytogenes). The zein-based films with the incorporation of licorice essential oil presented the typical rugosities of the lotus leaf making the surfaces very hydrophobic (water contact angle of 112.50°). In addition to having antioxidant and antibacterial properties, the films also shown to be biodegradable, making them a strong alternative to the traditional plastics used in food packaging.

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          Most cited references 37

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          Listeria monocytogenes: towards a complete picture of its physiology and pathogenesis

          Listeria monocytogenes is a food-borne pathogen responsible for a disease called listeriosis, which is potentially lethal in immunocompromised individuals. This bacterium, first used as a model to study cell-mediated immunity, has emerged over the past 20 years as a paradigm in infection biology, cell biology and fundamental microbiology. In this Review, we highlight recent advances in the understanding of human listeriosis and L. monocytogenes biology. We describe unsuspected modes of hijacking host cell biology, ranging from changes in organelle morphology to direct effects on host transcription via a new class of bacterial effectors called nucleomodulins. We then discuss advances in understanding infection in vivo, including the discovery of tissue-specific virulence factors and the 'arms race' among bacteria competing for a niche in the microbiota. Finally, we describe the complexity of bacterial regulation and physiology, incorporating new insights into the mechanisms of action of a series of riboregulators that are critical for efficient metabolic regulation, antibiotic resistance and interspecies competition.
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            Polysaccharide-based films and coatings for food packaging: A review

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              Use of essential oils in active food packaging: Recent advances and future trends

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                Author and article information

                Journal
                Microorganisms
                Microorganisms
                microorganisms
                Microorganisms
                MDPI
                2076-2607
                16 August 2019
                August 2019
                : 7
                : 8
                Affiliations
                [1 ]Centro de Investigação em Ciências da Saúde (CICS-UBI), Universidade da Beira Interior, Avenida Infante D. Henrique, 6200–506 Covilhã, Portugal
                [2 ]Laboratório de Fármaco-Toxicologia, UBIMedical, Universidade da Beira Interior, Estrada Municipal 506, 6200–284 Covilhã, Portugal
                [3 ]Departamento de Química, Faculdade de Ciências, Universidade da Beira Interior, Rua Marquês d’Ávila e Bolama, 6201–001 Covilhã, Portugal
                [4 ]Materiais Fibrosos e Tecnologias Ambientais (FibEnTech), Universidade da Beira Interior, Rua Marquês d’Ávila e Bolama, 6201–001 Covilhã, Portugal
                Author notes
                [* ]Correspondence: ammr@ 123456ubi.pt ; Tel.: +351-275-319-700
                Article
                microorganisms-07-00267
                10.3390/microorganisms7080267
                6723050
                31426406
                9613ed92-997e-416b-be59-61f7d1daeaf2
                © 2019 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

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