Journal ID (nlm-ta): PLoS One
Journal ID (iso-abbrev): PLoS ONE
Journal ID (publisher-id): plos
Journal ID (pmc): plosone
Title:
PLoS ONE
Publisher:
Public Library of Science
(San Francisco, CA USA
)
ISSN
(Electronic):
1932-6203
Publication date
(Electronic):
18
October
2016
Publication date Collection: 2016
Volume: 11
Issue: 10
Electronic Location Identifier: e0164041
Affiliations
[1
]Division of Respiratory Medicine, University of Nottingham, Nottingham, United Kingdom
[2
]Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden
[3
]Department of Biology, University of York, York, United Kingdom
[4
]Pfizer Worldwide Research & Development, Cambridge, Massachusetts, United States of
America
[5
]Pfizer Worldwide Research & Development, Cambridge, United Kingdom
[6
]Institute for Ageing and Health, Newcastle University, Campus for Ageing and Vitality,
Newcastle upon Tyne, United Kingdom
[7
]National Heart and Lung Institute, Imperial College London, London, United Kingdom
[8
]Institute for Lung Health, Immunity and Inflammation, University of Leicester, Leicester,
United Kingdom
University of Pittsburgh, UNITED STATES
Author notes
Competing Interests: AM and IK are employees of Pfizer Ltd. PPP Healthcare Medical Trust funded sample
collection for SGP for this publication. AJW is an advisory board member reporting
personal fees from GlaxoSmithKline, Boehringer Ingelheim and Pulmocide outside of
the submitted work. IK and AM's commercial affiliation with Pfizer does not alter
our adherence to PLOS ONE policies on sharing data and materials.
-
Conceptualization: IPH IS
-
Data curation: SM APH EH AKK CKB SKB MO EM SKM CS CG CPN CES
-
Formal analysis: SM APH EH AKK CKB SKB MO EM SKM CS CG CPN CES
-
Funding acquisition: IK AM IPH IS
-
Investigation: SM APH EH AKK CKB TLR SKB MO EM SKM CS CG CPN CES
-
Methodology: SM APH EH AKK CKB SKB EM CS CES IPH IS
-
Project administration: SM IPH IS
-
Resources: EM CEB SGP MFM AJW IPH IS
-
-
Validation: SM APH EH CKB TLR CS CG CPN CES
-
Visualization: SM EH AKK SKB MO EM SKM CS CES
-
Writing – original draft: SM IPH IS
-
Writing – review & editing: SM APH EH AKK CKB TLR SKB MO EM SKM CS CG CPN CES CEB IK AM SGP MFM AJW IPH IS.
[¤a]
Current address: James Hogg Research Centre, Institute for Heart and Lung Health,
University of British Columbia, Vancouver, British Columbia, Canada
[¤b]
Current address: Sachs’ Children’s Hospital, Stockholm, Sweden
Article
Publisher ID:
PONE-D-16-16828
DOI: 10.1371/journal.pone.0164041
PMC ID: 5068780
PubMed ID: 27755550
SO-VID: 9674aa52-247f-4624-8717-d41ca9520236
Copyright © © 2016 Miller et al
License:
This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
the original author and source are credited.
Funded by: funder-id http://dx.doi.org/10.13039/501100000265, Medical Research Council;
Award ID: G1000861
Award Recipient
:
Ian P. Hall
Funded by: funder-id http://dx.doi.org/10.13039/100009032, Pfizer UK;
Award Recipient
:
Ian P. Hall
Funded by: The Swedish Research Council
Award Recipient
:
Erik Melén
Funded by: Swedish Heart-Lung Foundation
Award Recipient
:
Erik Melén
Funded by: Stockholm County Council
Award Recipient
:
Erik Melén
Funded by: funder-id http://dx.doi.org/10.13039/501100000272, National Institute for Health Research;
Award Recipient
:
Andrew J. Wardlaw
This study was funded by grants from the Medical Research Council, UK (G1000861) and
Pfizer Inc. EM has received funding from The Swedish Research Council, The Swedish
Heart-Lung Foundation and Stockholm County Council (ALF). AJW is supported by the
National Institute for Health Research Leicester Respiratory Biomedical Research Unit.
The views expressed are those of the authors and not necessarily those of the NHS,
the NIHR or the Department of Health. The human embryonic and fetal material was provided
by the Joint MRC/Wellcome Trust (grant # 099175/Z/12/Z) Human Developmental Biology
Resource (
www.hdbr.org). The funder (Pfizer) provided support in the form of salaries for authors [IK and
AM], but did not have any additional role in the study design, data collection and
analysis, decision to publish, or preparation of the manuscript. The specific roles
of these authors are articulated in the 'author contributions' section.
Subject:
Research Article
Subject:
Biology and Life Sciences
Subject:
Developmental Biology
Subject:
Organism Development
Subject:
Organogenesis
Subject:
Lung Development
Subject:
Medicine and Health Sciences
Subject:
Pulmonology
Subject:
Chronic Obstructive Pulmonary Disease
Subject:
Biology and Life Sciences
Subject:
Genetics
Subject:
Gene Expression
Subject:
Biology and Life Sciences
Subject:
Cell Biology
Subject:
Cellular Types
Subject:
Animal Cells
Subject:
Epithelial Cells
Subject:
Biology and Life Sciences
Subject:
Anatomy
Subject:
Biological Tissue
Subject:
Epithelium
Subject:
Epithelial Cells
Subject:
Medicine and Health Sciences
Subject:
Anatomy
Subject:
Biological Tissue
Subject:
Epithelium
Subject:
Epithelial Cells
Subject:
Biology and life sciences
Subject:
Molecular biology
Subject:
Molecular biology techniques
Subject:
Sequencing techniques
Subject:
RNA sequencing
Subject:
Research and analysis methods
Subject:
Molecular biology techniques
Subject:
Sequencing techniques
Subject:
RNA sequencing
Subject:
Research and analysis methods
Subject:
Extraction techniques
Subject:
RNA extraction
Subject:
Biology and life sciences
Subject:
Biochemistry
Subject:
Nucleic acids
Subject:
RNA
Subject:
Messenger RNA
Subject:
Biology and Life Sciences
Subject:
Anatomy
Subject:
Biological Tissue
Subject:
Epithelium
Subject:
Medicine and Health Sciences
Subject:
Anatomy
Subject:
Biological Tissue
Subject:
Epithelium