Microbial models of mammalian metabolism. Furosemide glucoside formation using the fungus Cunninghamella elegans.

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Abstract

The diuretic furosemide (Lasix) was metabolized by the fungus Cunninghamella elegans (ATCC 36112) to the phase II conjugate, furosemide acyl glucoside. This metabolite was isolated following semipreparative scale incubations of C. elegans involving glucose nutrient dosing, and was characterized by NMR spectroscopy (1H and 1H/1H correlated), MS (FAB), UV, HPLC with fluorescence detection, and enzymatic treatments. The aglycone fragment of the conjugate was characterized as furosemide by treatment of the metabolite with sodium hydroxide, whereas the sugar part was identified as glucose by cleavage of the conjugate, derivatization of the released sugar, and GC/MS analysis.

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Journal

Journal ID (iso-abbrev): Drug Metab. Dispos.

Title: Drug metabolism and disposition: the biological fate of chemicals

ISSN: 0090-9556

ISSN (Print): 0090-9556

Publication date (Electronic): March 1 1993

Volume: 21

Issue: 2

Affiliations

[1 ] Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Texas, Austin 78712-1074.

Article

PubMed ID: 8097695

SO-VID: 967f1a9d-a635-4d9c-a732-aee1dd533c8b

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