Dietary replacement of soybean meal by fermented feedstuffs for aged laying hens: effects on laying performance, egg quality, nutrient digestibility, intestinal health, follicle development, and biological parameters in a long-term feeding period

Abstract

This study aimed to investigate the effects of dietary supplementation with fermented soybean meal ( FSM) or fermented miscellaneous meal ( FMM, cottonseed meal: coconut meal = at a 1:1 ratio) on the intestinal health, laying performance, egg quality, and follicle development of laying hens. A total of 1,008 54-wk-old laying hens were randomly divided into 7 treatment groups and fed a corn-soybean base diet in addition to 2%, 4%, and 8% FSM or FMM. The results showed that fermentation increased the contents of crude protein, amino acids (Ser, Gly, Cys, Leu, Lys, His, and Arg), and organic acids (butyric acid, citric acid, succinic acid) and decreased the contents of neutral and acid detergent fiber in the soybean and miscellaneous meals ( P < 0.05). Compared with the results found for the control group, feeding with 4% FSM increased the egg production, egg mass and average daily feed intake ( ADFI), and feeding with 4% FMM increased the ADFI of laying hens ( P < 0.05). Furthermore, feeding with 8% FMM reduced the productive performance and laying performance, supplementation with 4% FSM increased the eggshell strength and weight, and 2 to 4% FSM increased the egg albumen height and Haugh unit ( P < 0.05). Moreover, 2 to 8% FSM or 2 to 4% FMM enhanced the apparent digestibility of dry matter, crude protein, and NDF for laying hens ( P < 0.05). The relative weight, villus height, crypt depth, and villus:crypt ratio of the jejunum were higher in the 4% FSM- and FMM-fed groups ( P < 0.05). Moreover, diamine oxidase ( DAO) activity, transepithelial electrical resistance ( TEER), and the expression of tight junction proteins (ZO-1, Occluding, and Claudin1), the intestinal stem cell marker Lgr5, and the proliferation cell marker proliferating cell nuclear antigen ( PCNA) was upregulated in the jejunum of laying hens fed 4% FSM and FMM ( P < 0.05). The relative weight of the ovaries, and the number of small yellow follicles and large white follicles were elevated after 4% FSM or FMM supplementation. Furthermore, the levels of serum follicle-stimulating hormone and luteinizing hormone were increased in the 4% FSM and FMM groups ( P < 0.05). In conclusion, the supplementation of laying hen feed with FSM and FMM improved the laying performance, egg quality, intestinal barrier function, and follicle development of aged laying hens, and 4% FSM supplementation was optimal.