11
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Genetic characterization and authentication of Dimocarpus longan Lour. using an improved RAPD technique.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Dimocarpus longan Lour. is an edible and traditional herb in China, commonly referred to as longon. An improved randomly amplified polymorphic DNA (RAPD) protocol was here developed in order to determine the geographical origins of D. longan samples collected from 5 provinces in the southern and southwestern areas of China, including Sichuan, Hainan, Fujian, Guangdong, and Guangxi. Generally, the improved RAPD method generated good fingerprinting of the 5 samples using the selected 17 primers. In particular, primers SBS-A5, SBS-A13, SBS-I9, SBS-I20, SBS-M1, and SBS-Q12 produced distinguishable bands that clearly separated all 5 cultivars, suggesting that there are variations in RAPD genetic sites among the samples. The similarity index ranged from 0.69 to 0.76. The Sichuan and Hainan clades clustered together with a 0.73 similarity index. The Guangxi and Fujian clades clustered together with a 0.76 similarity index, and they formed the sister clade to the Sichuan/Hainan clade with a 0.71 similarity index. The Guangdong clade was in a basal polytomy with a 0.70 similarity index. Based on the abundant DNA polymorphisms, these longan accessions are distinguishable using our improved RAPD technique. Therefore, RAPD analysis is an effective technique in distinguishing the geographical origins of D. longan. Moreover, the improved method could also be employed for a variety of applications including genetic diversity and fingerprinting analyses.

          Related collections

          Author and article information

          Journal
          Genet. Mol. Res.
          Genetics and molecular research : GMR
          Genetics and Molecular Research
          1676-5680
          1676-5680
          Mar 06 2014
          : 13
          : 1
          Affiliations
          [1 ] Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou, Sichuan Province, China.
          [2 ] Michael E. DeBakey High School for Health Professions, Houston, TX, USA.
          [3 ] Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou, Sichuan Province, China fujunjiang@hotmail.com.
          Article
          gmr2858
          10.4238/2014.March.6.3
          24634243
          96b7facc-78a4-486f-a1b5-dc342305f5b9
          History

          Comments

          Comment on this article