Acute renal necrosis of the distal or straight portion of the proximal convoluted tubule was induced in the mouse kidney with an intraperitoneal injection of uranyl nitrate. Regeneration of the tubular epithelium occurred via mitotic proliferation of the cells in a zone of dedifferentiation which developed at the cortico-medullary interface with migration of the cells proximally up the nephron. Re-epifhelialization was essentially complete within five days of the injection. The new cells at first developed organelles associated with synthesis of protein, i.e. RNP particles, rough surfaced ER, prominent Golgi apparatus and increased numbers of nuclear pores. Once the tubular cell mass has been reconstituted, this specialized machinery was replaced over the course of the first 7–10 days by microvilli, large numbers of mitochondria and basal infoldings thus adapting the cell for the process of re-absorption. No acute glomerular lesions and only rare chronic tubular alterations were seen.