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      An Inexpensive, Fast and Sensitive Quantitative Lateral Flow Magneto-Immunoassay for Total Prostate Specific Antigen

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          Abstract

          We describe the detection characteristics of a device the Resonant Coil Magnetometer (RCM) to quantify paramagnetic particles (PMPs) in immunochromatographic (lateral flow) assays. Lateral flow assays were developed using PMPs for the measurement of total prostate specific antigen (PSA) in serum samples. A detection limit of 0.8 ng/mL was achieved for total PSA using the RCM and is at clinically significant concentrations. Comparison of data obtained in a pilot study from the analysis of serum samples with commercially available immunoassays shows good agreement. The development of a quantitative magneto-immunoassay in lateral flow format for total PSA suggests the potential of the RCM to operate with many immunoassay formats. The RCM has the potential to be modified to quantify multiple analytes in this format. This research shows promise for the development of an inexpensive device capable of quantifying multiple analytes at the point-of-care using a magneto-immunoassay in lateral flow format.

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          Most cited references38

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          Point of care diagnostics: status and future.

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            Practical Statistics for Medical Research

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              Advances in paper-based point-of-care diagnostics.

              Advanced diagnostic technologies, such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), have been widely used in well-equipped laboratories. However, they are not affordable or accessible in resource-limited settings due to the lack of basic infrastructure and/or trained operators. Paper-based diagnostic technologies are affordable, user-friendly, rapid, robust, and scalable for manufacturing, thus holding great potential to deliver point-of-care (POC) diagnostics to resource-limited settings. In this review, we present the working principles and reaction mechanism of paper-based diagnostics, including dipstick assays, lateral flow assays (LFAs), and microfluidic paper-based analytical devices (μPADs), as well as the selection of substrates and fabrication methods. Further, we report the advances in improving detection sensitivity, quantification readout, procedure simplification and multi-functionalization of paper-based diagnostics, and discuss the disadvantages of paper-based diagnostics. We envision that miniaturized and integrated paper-based diagnostic devices with the sample-in-answer-out capability will meet the diverse requirements for diagnosis and treatment monitoring at the POC. © 2013 Elsevier B.V. All rights reserved.
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                Author and article information

                Journal
                Biosensors (Basel)
                Biosensors (Basel)
                biosensors
                Biosensors
                MDPI
                2079-6374
                08 July 2014
                September 2014
                : 4
                : 3
                : 204-220
                Affiliations
                [1 ]Institute of Bio-Sensing Technology, University of the West of England, Bristol, BS16 1QY, UK; E-Mails: jackie.barnett@ 123456uwe.ac.uk (J.M.B.); patrickwraith@ 123456blueyonder.co.uk (P.W.); janice.kiely@ 123456uwe.ac.uk (J.K.); hp@ 123456hphawkins.com (P.H.)
                [2 ]Bristol Urological Institute, Southmead Hospital, Bristol, BS10 5NB, UK; E-Mail: rajpersad@ 123456bristolurology.com
                [3 ]Department of Surgery, University Hospitals Bristol NHS Trust, Upper Maudlin Street, Bristol, BS2 8HW, UK; E-Mail: Katrina.Hurley@ 123456uhbristol.nhs.uk
                Author notes
                [†]

                These authors contributed equally to this work.

                [* ]Author to whom correspondence should be addressed; E-Mail: richard.luxton@ 123456uwe.ac.uk ; Tel.: +44-117-328-2472; Fax: +44-117-328-2904.
                Article
                biosensors-04-00204
                10.3390/bios4030204
                4264355
                970f382f-0159-4cbb-85d3-ed1fdc39a7e9
                © 2014 by the authors; licensee MDPI, Basel, Switzerland.

                This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/3.0/).

                History
                : 08 April 2014
                : 09 June 2014
                : 13 June 2014
                Categories
                Article

                paramagnetic particles,resonant coil magnetometer,prostate specific antigen

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