7
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Massively parallel sequencing, a new method for detecting adventitious agents.

      Biologicals : journal of the International Association of Biological Standardization
      Algorithms, Animals, Cell Line, Gene Expression Profiling, Genome, Viral, genetics, Humans, Sequence Analysis, DNA, methods, Virion, Viruses, classification, isolation & purification

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          There has been an upsurge of interest in developing new veterinary and human vaccines and, in turn, this has involved the development of new mammalian and insect cell substrates. Excluding adventitious agents from these cells can be problematic, particularly for cells derived from species with limited virological investigation. Massively parallel sequencing is a powerful new method for the identification of viruses and other adventitious agents, without prior knowledge of the nature of the agent. We have developed methods using random priming to detect viruses in the supernatants from cell substrates or in virus seed stocks. Using these methods we have recently discovered a new parvovirus in bovine serum. When applied to sequencing the transcriptome, massively parallel sequencing can reveal latent or silent infections. Enormous amounts of data are developed in this process usually between 100 and 400 Mbp. Consequently, sophisticated bioinformatic algorithms are required to analyse and verify virus targets. 2010. Published by Elsevier Ltd.

          Related collections

          Author and article information

          Journal
          20338780
          10.1016/j.biologicals.2010.01.003

          Chemistry
          Algorithms,Animals,Cell Line,Gene Expression Profiling,Genome, Viral,genetics,Humans,Sequence Analysis, DNA,methods,Virion,Viruses,classification,isolation & purification

          Comments

          Comment on this article