10
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Correction: A rapid multiplex PCR assay for presumptive species identification of rhinoceros horns and its implementation in Vietnam

      correction

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          There are errors in Table 1. The amplicon lengths for primers RID_FWD/RID_REV and Mac_FWD/Mac_REV do not include primer regions. Please see the corrected Table 1 here. 10.1371/journal.pone.0202433.t001 Table 1 Cytochrome b markers and their corresponding primers. Genetic marker for: Primer name: Primer sequence (5’–3’): Annealing temperature (°C): Amplicon length* (bp): Reference: Diceros bicornis (black rhino) Rh_BR_FWD (forward) AATCTGCCTAATCCTACAAATC 60 222 This study Rh_BR_REV (reverse) GGTTTCTAGGAAGGTGTAGG Ceratotherium simum (white rhino) Rh_WR_FWD (forward) CCACTCATTCATCGATCTGC 60 266 This study Rh_WR_REV (reverse) TAATAGATACCGCGTCCTAC Rhinoceros unicornis (Indian rhino) Rh_IR_FWD (forward) TCTCACCCACTAGTTAAAATCA 60 310 This study Rh_IR_REV (reverse) AGGAAGGTGTAAGATCCATAG All rhino species RID_FWD (forward) AACATCCGTAAATCYCACCCA 55 274 [6] RID_REV (reverse) GGCAGATRAARAATATGGATGCT All rhino species Mac_FWD (forward CAYTATACACCAGACACAACAAC 55 226 This study Mac_REV (reverse) TGAAYGCDGTGGCTATTAGRG *Amplicon lengths are inclusive of primer regions.

          Related collections

          Most cited references1

          • Record: found
          • Abstract: found
          • Article: found
          Is Open Access

          A rapid multiplex PCR assay for presumptive species identification of rhinoceros horns and its implementation in Vietnam

          Rhinoceros (rhinos) have suffered a dramatic increase in poaching over the past decade due to the growing demand for rhino horn products in Asia. One way to reverse this trend is to enhance enforcement and intelligence gathering tools used for species identification of horns, in particular making them fast, inexpensive and accurate. Traditionally, species identification tests are based on DNA sequence data, which, depending on laboratory resources, can be either time or cost prohibitive. This study presents a rapid rhino species identification test, utilizing species-specific primers within the cytochrome b gene multiplexed in a single reaction, with a presumptive species identification based on the length of the resultant amplicon. This multiplex PCR assay can provide a presumptive species identification result in less than 24 hours. Sequence-based definitive testing can be conducted if/when required (e.g. court purposes). This work also presents an actual casework scenario in which the presumptive test was successfully utlitised, in concert with sequence-based definitive testing. The test was carried out on seized suspected rhino horns tested at the Institute of Ecology and Biological Resources, the CITES mandated laboratory in Vietnam, a country that is known to be a major source of demand for rhino horns. This test represents the basis for which future ‘rapid species identification tests’ can be trialed.
            Bookmark

            Author and article information

            Journal
            PLoS One
            PLoS ONE
            plos
            plosone
            PLoS ONE
            Public Library of Science (San Francisco, CA USA )
            1932-6203
            10 August 2018
            2018
            10 August 2018
            : 13
            : 8
            : e0202433
            Article
            PONE-D-18-23001
            10.1371/journal.pone.0202433
            6086477
            30096182
            972a1636-8ded-4c01-b4e8-014f1687973a
            © 2018 Ewart et al

            This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

            History
            Page count
            Figures: 0, Tables: 1, Pages: 2
            Categories
            Correction

            Uncategorized
            Uncategorized

            Comments

            Comment on this article