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      Detección del virus de la hoja amarilla de la caña de azúcar y virus mosaico de la caña de azúcar en sorgo (Sorghum bicolor (L.) Moench) en el Estado de Morelos, México Translated title: Detection of sugarcane yellow leaf virus and sugarcane mosaic virus in sorghum (Sorghum bicolor (L.) Moench) in the state of Morelos, México

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          Abstract

          El presente estudio se realizó en los meses de junio-julio del 2017, con el propósito de determinar la presencia de virus asociados al cultivo del sorgo (Sorghum bicolor (L.) Moench). Se muestrearon 21 localidades, distribuidas en 10 municipios de los 33 que forman el Estado de Morelos. Se utilizaron dos anticuerpos para detectar las enfermedades Virus de la hoja amarilla de la caña de azúcar (SCYLV) y Virus mosaico de la caña de azúcar (SCMV). Se analizaron muestras de follaje colectadas al azar en cinco puntos de cada campo o parcela. El diagnóstico se realizó mediante el test inmunoenzimático tipo DAS-ELISA (doble anticuerpo), con un set de reactivos AGDIA (2017), con conjugado de fosfatasa alcalina para (SCMV) y el segundo set de reactivos de NANO Diagnostic (2017) "AC Diagnostic" con conjugado fosfatasa alcalina para (SCYLV). Los resultados obtenidos evidenciaron presencia de ambas enfermedades virales en el cultivo del sorgo para el estado de Morelos. Con muestras positivas al (SCYLV) y absorbancias altas (1,50 A° y 1,10 A°) en los municipios de Amacuzac y Tequesquitengo respectivamente. Mientras solo dos muestras positivas al (SCMV) fueron detectadas en las localidades de Puente de Ixtla y Yautepec, pero con valores bajos de absorbancia respecto a los controles positivos

          Translated abstract

          The study was carried out in the months of June-July 2017, with the purpose of studying the current situation of the viruses associated with the cultivation of sorghum (Sorghum bicolor (L.) Moench). Twenty-one commercial sorghum plantation in Morelos state, were analyzed to detected Sugarcane yellow leaf virus (SCYLV) and Sugarcane mosaic virus (SCMV). The diagnosis was made, by means DAS-ELISA (double antibody) an immunoenzymatic test, that used a AGDIA kit (2017) and a reagent set of NANO Diagnostic (2017) "AC Diagnostic" with conjugated alkaline phosphatase, to detection of SCMV and SCYLV respectively. The results obtained evidenced, the presence of both viral diseases in the sorghum cultivation, in ten municipalities of Morelos state whit predominance of SCYLV. Positives samples to this virus with high absorbance (1.50 A° and 1.10 A°) on the municipalities of Amacuzac and Tequesquitengo, respectively were observed. While, only two positive samples of SCMV, in Puente de Ixtla and Yautepec were detected, but with low absorbance values respect to positive controls

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          Most cited references13

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          Molecular Variability and Distribution of Sugarcane Mosaic Virus in Shanxi, China

          Background Sugarcane mosaic virus (SCMV) is responsible for large-scale economic losses in the global production of sugarcane, maize, sorghum, and some other graminaceous species. To understand the evolutionary mechanism of SCMV populations, this virus was studied in Shanxi, China. A total of 86 maize leaf samples (41 samples in 2012 and 45 samples in 2013) were collected from 4 regions of Shanxi. Results Double-antibody sandwich (DAS)-ELISA and RT-PCR showed 59 samples (30 samples in 2012 and 29 samples in 2013) to be positive for SCMV, from which 10 new isolates of SCMV were isolated and sequenced. The complete genomes of these isolates are 9610 nt long, including the 5′ and 3′ non-coding regions, and encode a 3063-amino acid polyprotein. Phylogenetic analyses revealed that 24 SCMV isolates could be divided on the basis of the whole genome into 2 divergent evolutionary groups, which were associated with the host species. Among the populations, 15 potential recombination events were identified. The selection pressure on the genes of these SCMV isolates was also calculated. The results confirmed that all the genes were under negative selection. Conclusions Negative selection and recombination appear to be important evolutionary factors shaping the genetic structure of these SCMV isolates. SCMV is distributed widely in China and exists as numerous strains with distinct genetic diversity. Our findings will provide a foundation for evaluating the epidemiological characteristics of SCMV in China and will be useful in designing long-term, sustainable management strategies for SCMV.
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            Simultaneous detection and identification of four sugarcane viruses by one-step RT-PCR

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              The complete genome sequences of two naturally occurring recombinant isolates of Sugarcane mosaic virus from Iran.

              Sugarcane mosaic virus (SCMV) is the most prevalent virus causing sugarcane mosaic and maize dwarf mosaic diseases. Here, we presented the first two complete genomic sequences of Iranian SCMV isolates, NRA and ZRA from sugarcane and maize. The complete genome sequences of NRA and ZRA were, respectively, 9571 and 9572 nucleotides (nt) in length, excluding the 3'-terminal poly(A) tail. Both isolates contained a 5'-untranslated region (UTR) of 149 nt, an open reading frame of 9192 nt encoding a polyprotein of 3063 amino acids (aa), and 3'-UTR of 230 nt for NRA and 231 nt for ZRA. SCMV-NRA and -ZRA genome nucleotide sequences were 97.3 % identical and shared nt identities of 79.1-92 % with those of other 21 SCMV isolates available in the GenBank, highest with the isolate Bris-A (AJ278405) (92 and 91.7 %) from Australia. When compared for separate genes, most of their genes shared the highest identities with Australian and Argentinean isolates. Phylogenetic analysis of the complete genomic sequences reveals that SCMV can be clustered to three groups. Both NRA and ZRA were clustered with sugarcane isolates from Australia and Argentina in group III but formed a separate sublineage. Recombination analysis showed that both isolates were intraspecific recombinants, and represented two novel recombination patterns of SCMV (in the P1 coding region). NRA had six recombination sites within the P1, HC-Pro, CI, NIa-Vpg, and NIa-pro coding regions, while ZRA had four within the P1, HC-Pro, NIa-Pro, and NIb coding regions.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
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                Role: ND
                Journal
                agro
                Scientia Agropecuaria
                Scientia Agropecuaria
                Universidad Nacional de Trujillo. Facultad de Ciencias Agropecuarias (Trujillo, , Peru )
                2077-9917
                July 2018
                : 9
                : 3
                : 423-427
                Affiliations
                [03] Ciudad Ayala Morelos orgnameUAEM orgdiv1Escuela de Estudios Superiores de Xalostoc México
                [04] Cuernavaca Morelos orgnameUAEM orgdiv1Facultad de Ciencias Agropecuarias
                [01] Cuernavaca Morelos orgnameUAEM orgdiv1Facultad de Ciencias Agropecuarias
                [02] Cuautla Morelos orgnameLaboratorio de Agrodiagnóstico Fitolab México
                Article
                S2077-99172018000300014
                10.17268/sci.agropecu.2018.03.14
                978b81bd-9b2f-487b-a349-c521b14747bb

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 16 May 2018
                : 13 June 2018
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 19, Pages: 5
                Product

                SciELO Peru

                Categories
                Comunicación Corta

                sorgo,sorghum,virus,DAS-ELISA
                sorgo, sorghum, virus, DAS-ELISA

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