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      Media Conditioned by Coculture of Pericytes and Endothelial Cells under a Hypoxic State Stimulate in vitro Angiogenesis

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          Abstract

          We investigated the role of pericytes in retinal neovascularization and the effect of hypoxia on it. Conditioned media (CM) were harvested from cultured pericytes, retinal capillary endothelial cells (RCEs) or cocultured pericytes and RCEs at 1 5 and 20% O<sub>2</sub>. The effect of these CM on the capillary-like tube formation of RCEs in the collagen gel was then investigated. The CM harvested from either pericytes or coculture of pericytes and RCEs in a hypoxic environment (1 and 5 % O<sub>2</sub>) significantly enhanced the tube formation of RCEs (p < 0.01), while the CM of coculture at 20% O<sub>2</sub> showed an inhibitory effect (p < 0.01). The present study suggests that pericytes release angiogenic factors under hypoxic conditions, i.e. at 1 and 5% O<sub>2</sub>, while they release angiostatic factors when they are cocultured with RCEs at 20% O<sub>2</sub>.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1994
          1994
          11 December 2009
          : 26
          : 1
          : 23-31
          Affiliations
          Departments of aPathology and bOphthalmology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
          Article
          267370 Ophthalmic Res 1994;26:23–31
          10.1159/000267370
          8134086
          © 1994 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 9
          Categories
          Original Paper

          Vision sciences, Ophthalmology & Optometry, Pathology

          Hypoxia, Neovascularization, Retina, Endothelium, Pericyte

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