4
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Simultaneous quantitative determination of arachidonic acid and cascade metabolites in rat serum by UPLC-MS/MS: application for longitudinal metabolomics of anlotinib

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Robust UPLC/MS/MS method was firstly developed and validated; arachidonic acid and cascade metabolites have been shown to be associated with anlotinib longitudinal metabolomics.

          Abstract

          Arachidonic acid (AA) and cascade metabolites have been shown to be involved in cancer pathologic states. Anlotinib, a novel oral small molecule inhibitor of multiple receptor tyrosine kinases, has brought significant improvement to the survival of patients with advanced lung cancer. Here, a robust and reproducible ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed, optimized and validated for quantitating AA and cascade metabolites for the first time. Through careful optimization of the analytical conditions, a total of 69 analytes can be efficiently separated and quantitated in a single run of 17 min. A simple and labor-saving protein precipitation procedure was utilized for serum sample pretreatment. The validation parameters and quality control chart of all analytes satisfy the acceptance criteria of bioanalytical method guidelines. The limit of detection (LOD) ranged from 0.005 ng mL −1 to 1 ng mL −1, and the volume of serum was only 20 μL. This rapid and sensitive UPLC-MS/MS method was successfully applied to a longitudinal metabolomics study in rat serum after a single administration of anlotinib (6 mg kg −1). Finally, a total of 41 metabolites can be calculated under the present conditions. Serum samples from the same rat were segregated into a tight cluster in both unsupervised principal component analysis (PCA) and supervised orthogonal partial least-squares discriminant analysis (OPLS-DA) at different sampling time points after anlotinib treatment. Moreover, the number of analytes whose variable importance (VIP) values were larger than 1.0 was 17. The present study not only offers a UPLC-MS/MS analytical reference for AA but also brings out insights for future mechanistic studies or biomarkers to predict the efficacy, toxicity and clinical outcomes in patients with cancer.

          Related collections

          Most cited references1

          • Record: found
          • Abstract: not found
          • Book: not found

          The Pharmacopoeia of the People's Republic of China, 2015 Edition Part IV

          Chinese (2015)
            Bookmark

            Author and article information

            Contributors
            Journal
            ANALAO
            The Analyst
            Analyst
            Royal Society of Chemistry (RSC)
            0003-2654
            1364-5528
            July 13 2020
            2020
            : 145
            : 14
            : 4972-4981
            Affiliations
            [1 ]Beijing Chao-Yang Hospital
            [2 ]Capital Medical University
            [3 ]Beijing
            [4 ]China
            Article
            10.1039/D0AN00867B
            97e88522-afb7-4825-a71c-2dbd7d0c7eab
            © 2020

            http://rsc.li/journals-terms-of-use

            History

            Comments

            Comment on this article