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      Target-decoy search strategy for mass spectrometry-based proteomics.

      Methods in Molecular Biology (Clifton, N.j.)
      Algorithms, Databases, Protein, Mass Spectrometry, methods, Proteins, analysis, Proteomics, Software

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          Abstract

          Accurate and precise methods for estimating incorrect peptide and protein identifications are crucial for effective large-scale proteome analyses by tandem mass spectrometry. The target-decoy search strategy has emerged as a simple, effective tool for generating such estimations. This strategy is based on the premise that obvious, necessarily incorrect "decoy" sequences added to the search space will correspond with incorrect search results that might otherwise be deemed to be correct. With this knowledge, it is possible not only to estimate how many incorrect results are in a final data set but also to use decoy hits to guide the design of filtering criteria that sensitively partition a data set into correct and incorrect identifications.

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          Author and article information

          Journal
          20013364
          2922680
          10.1007/978-1-60761-444-9_5

          Chemistry
          Algorithms,Databases, Protein,Mass Spectrometry,methods,Proteins,analysis,Proteomics,Software
          Chemistry
          Algorithms, Databases, Protein, Mass Spectrometry, methods, Proteins, analysis, Proteomics, Software

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