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      Is Open Access

      Influence of Lung Parenchyma Surgical Manipulation on Circulating Free DNA

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          Transitions between epithelial and mesenchymal states: acquisition of malignant and stem cell traits.

          Transitions between epithelial and mesenchymal states have crucial roles in embryonic development. Emerging data suggest a role for these processes in regulating cellular plasticity in normal adult tissues and in tumours, where they can generate multiple, distinct cellular subpopulations contributing to intratumoural heterogeneity. Some of these subpopulations may exhibit more differentiated features, whereas others have characteristics of stem cells. Owing to the importance of these tumour-associated phenotypes in metastasis and cancer-related mortality, targeting the products of such cellular plasticity is an attractive but challenging approach that is likely to lead to improved clinical management of cancer patients.
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            Global patterns of cancer incidence and mortality rates and trends.

            While incidence and mortality rates for most cancers (including lung, colorectum, female breast, and prostate) are decreasing in the United States and many other western countries, they are increasing in several less developed and economically transitioning countries because of adoption of unhealthy western lifestyles such as smoking and physical inactivity and consumption of calorie-dense food. Indeed, the rates for lung and colon cancers in a few of these countries have already surpassed those in the United States and other western countries. Most developing countries also continue to be disproportionately affected by cancers related to infectious agents, such as cervix, liver, and stomach cancers. The proportion of new cancer cases diagnosed in less developed countries is projected to increase from about 56% of the world total in 2008 to more than 60% in 2030 because of the increasing trends in cancer rates and expected increases in life expectancy and growth of the population. In this review, we describe these changing global incidence and mortality patterns for select common cancers and the opportunities for cancer prevention in developing countries. (c)2010 AACR.
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              Real time quantitative PCR.

              We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude). Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.
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                Author and article information

                Journal
                Journal of Circulating Biomarkers
                Journal of Circulating Biomarkers
                IntechOpen
                1849-4544
                1849-4544
                January 2014
                January 2014
                January 2014
                January 2014
                : 3
                : 7
                Affiliations
                [1 ] University of Rome SAPIENZA, Department of Thoracic Surgery, Rome, Italy
                [2 ] University of Rome SAPIENZA, Department of Pathology, Rome, Italy
                [3 ] Fondazione Eleonora Lorillard Spencer Cenci, Rome, Italy
                Article
                10.5772/59875
                981c6566-7eaf-4272-8ac2-f62314919f9a
                © 2014

                http://journals.sagepub.com/page/policies/text-and-data-mining-license

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