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      Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[( R)-3-hydroxybutyramido]-D-glucose

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          Abstract

          Introduction:

          Hafnia alveiis the only species of the genus Hafnia, which belongs to the family of Enterobacteriaceae. These Gram-negative bacteria are commonly distributed in the natural environment and are often the cause of human opportunistic infections. Their lipopolysaccharides (LPSs) are important surface antigens which are responsible for the serological specificity and numerous cross-reactions with other enterobacterial genera. So far, 29 different O-polysaccharide (OPS, O-antigen) structures in Hafnias LPSs have been established and for some of them the molecular basis of the serological activity has been elucidated.

          Materials and Methods:

          OPS from H. alvei strain PCM 1219 was obtained by mild acid hydrolysis of the LPS followed by gel permeation chromatography of carbohydrate material on Sephadex G-50 column. The polysaccharide structure was determined using chemical methods as well as 13C NMR and 1H NMR spectroscopy. For serological studies, SDS-PAGE, immunoblotting, and passive hemagglutination tests were used.

          Results:

          The serological studies revealed a cross-reactivity of the LPSs of H. alvei PCM 1219 and a group of H. alvei strains with an O-antigen containing D-glucose 1-phosphate and [ (R)-3-hydroxybutyramido]-D-glucose. The following structure of the OPS was established: where Acyl stands for ( R)-3-hydroxybutyryl and the degree of O-acetylation is ~70%. The structure of the core oligosaccharide was found to be typical of the genus Hafnia.

          Conclusions:

          Based on the OPS structure and serological results it was concluded that H. alvei strain PCM 1219 should be classified in the same serogroup as the H. alvei type strain ATCC 13337 and five other strains containing D-glucose 1-phosphate and 2-deoxy-2-[( R)-3-hydroxybutyramido]-D-glucose in their O-antigens.

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          Most cited references29

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          A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.

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            Carbohydrate structural determination by NMR spectroscopy: modern methods and limitations.

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              The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine, and its analytical application.

              S Hestrin (1949)
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                Author and article information

                Contributors
                +48 71 337-13-82 , katzenel@iitd.pan.wroc.pl
                Journal
                Arch Immunol Ther Exp (Warsz)
                Archivum Immunologiae et Therapiae Experimentalis
                Birkhäuser-Verlag (Basel )
                0004-069X
                1661-4917
                6 October 2008
                October 2008
                : 56
                : 5
                : 347-352
                Affiliations
                [1 ]Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland
                [2 ]N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
                [3 ]Department of Medical Biochemistry, Wrocław Medical University, Wrocław, Poland
                Article
                34
                10.1007/s00005-008-0034-1
                2768797
                18836891
                9829d2f8-6562-4430-b3d3-f6719af21293
                © Birkhaueser 2008
                History
                Categories
                Original Article
                Custom metadata
                © Birkhaueser 2008

                Immunology
                hafnia alvei,enterobacteria,o-antigen,bacterial polysaccharide structure,serological cross-reactivity,lipopolysaccharide

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