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      Corneal Cryopreservation Evaluated by Trypane Blue Staining

      Ophthalmic Research

      S. Karger AG

      Corneal cryopreservation, Corneal graft, Corneal staining, Trypane blue, Bovine cornea

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          Abstract

          Bovine corneal tissue was employed in the study of variable factors in a method of deep-freezing, storage and thawing, using Trypane blue staining as indicator of cellular damage. Variations in cell population density in the endothelial sheet were studied as a prerequisite for the evaluation of damage during the successive stages of the freezing procedures. 90% of the cells in the endothelial sheet survived freezing to ––160°C when the corneal buttons were passed through increasing concentrations of DMSO in serum and frozen at a certain rate in 1 ml 8% DMSO. The final concentration of DMSO, the amount of fluid surrounding the buttons during freezing and the duration of the isotermic part of the freezing curve were found to be critical.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1974
          1974
          27 November 2009
          : 6
          : 1
          : 23-35
          Affiliations
          Department of Ophthalmology and Tissue-Typing Laboratory, Århus Kommunehospital, University of Århus, Denmark
          Article
          264597 Ophthal Res 1974;6:23–35
          10.1159/000264597
          © 1974 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 13
          Categories
          Paper

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