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      Corneal Cryopreservation Evaluated by Trypane Blue Staining

      Ophthalmic Research

      S. Karger AG

      Corneal cryopreservation, Corneal graft, Corneal staining, Trypane blue, Bovine cornea

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          Bovine corneal tissue was employed in the study of variable factors in a method of deep-freezing, storage and thawing, using Trypane blue staining as indicator of cellular damage. Variations in cell population density in the endothelial sheet were studied as a prerequisite for the evaluation of damage during the successive stages of the freezing procedures. 90% of the cells in the endothelial sheet survived freezing to ––160°C when the corneal buttons were passed through increasing concentrations of DMSO in serum and frozen at a certain rate in 1 ml 8% DMSO. The final concentration of DMSO, the amount of fluid surrounding the buttons during freezing and the duration of the isotermic part of the freezing curve were found to be critical.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          27 November 2009
          : 6
          : 1
          : 23-35
          Department of Ophthalmology and Tissue-Typing Laboratory, Århus Kommunehospital, University of Århus, Denmark
          264597 Ophthal Res 1974;6:23–35
          © 1974 S. Karger AG, Basel

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          Page count
          Pages: 13


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