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      An in vitro Study of the Pharmacological Responses of Rat Middle Cerebral Artery: Effects of Overnight Storage

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          Abstract

          This study was designed to establish the in vitro pharmacological responses of rat middle cerebral arteries (MCA), and also their susceptibility to overnight cold storage at 4°C. MCAs were harvested from rats and pharmacological responses were studied using a Mulvany myograph. Responses to prostaglandin F2<sub>α</sub> (PGF<sub>2α</sub>), uridine triphosphate (UTP), noradrenaline (NA) and histamine were determined to investigate receptor-dependent responses, sodium nitroprusside and papaverine to investigate receptor-independent relaxation and L-arginine and Nω-Nitro- L-arginine methyl ester ( L-NAME) to examine nitric oxide synthase-dependent responses. Responses in fresh arteries were established and compared with responses in arteries which had been mounted on a myograph and stored overnight at 4°C before study the next day. The MCAs had an effective lumen diameter of 263 µm (SD 25) and sustained concentration-dependent contractions were produced by 124 m M K<sup>+</sup>, PGF<sub>2α</sub>, UTP and L-NAME. Sodium nitroprusside, NA, histamine, papaverine and L-arginine produced concentration-dependent relaxations in precontracted arteries. Overnight cold storage did not alter the pharmacological responses to any of the agonists tested.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1995
          1995
          24 September 2008
          : 32
          : 4
          : 230-236
          Affiliations
          Departments of aNeurosurgery and bExperimental Medicine and Pharmacology, Royal Hallamshire Hospital and University of Sheffield, UK
          Article
          159097 J Vasc Res 1995;32:230–236
          10.1159/000159097
          7654880
          © 1995 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 7
          Categories
          Research Paper

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