An in vitro Study of the Pharmacological Responses of Rat Middle Cerebral Artery: Effects of Overnight Storage

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Abstract

This study was designed to establish the in vitro pharmacological responses of rat middle cerebral arteries (MCA), and also their susceptibility to overnight cold storage at 4°C. MCAs were harvested from rats and pharmacological responses were studied using a Mulvany myograph. Responses to prostaglandin F2α (PGF2α), uridine triphosphate (UTP), noradrenaline (NA) and histamine were determined to investigate receptor-dependent responses, sodium nitroprusside and papaverine to investigate receptor-independent relaxation and L-arginine and Nω-Nitro- L-arginine methyl ester ( L-NAME) to examine nitric oxide synthase-dependent responses. Responses in fresh arteries were established and compared with responses in arteries which had been mounted on a myograph and stored overnight at 4°C before study the next day. The MCAs had an effective lumen diameter of 263 µm (SD 25) and sustained concentration-dependent contractions were produced by 124 m M K+, PGF2α, UTP and L-NAME. Sodium nitroprusside, NA, histamine, papaverine and L-arginine produced concentration-dependent relaxations in precontracted arteries. Overnight cold storage did not alter the pharmacological responses to any of the agonists tested.

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Journal

Journal ID (publisher-id): JVR

Journal ID (nlm-ta): J Vasc Res

Journal ID (doi): 10.1159/issn.1018-1172

Title: Journal of Vascular Research

Publisher: S. Karger AG

ISSN (Print): 1018-1172

ISSN (Electronic): 1423-0135

Publication date Subscription-year: 1995

Publication date (Print): 1995

Publication date (Electronic): 24 September 2008

Volume: 32

Issue: 4

Pages: 230-236

Affiliations

Departments of ^aNeurosurgery and ^bExperimental Medicine and Pharmacology, Royal Hallamshire Hospital and University of Sheffield, UK

Article

Publisher ID: 159097 Other ID: J Vasc Res 1995;32:230–236

DOI: 10.1159/000159097

PubMed ID: 7654880

SO-VID: 985a75f0-bb04-4436-85bb-8c910abf5e8d

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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 10 October 1994

Date accepted : 06 March 1995

Page count

Pages: 7

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