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      Observational Study Regarding the Relationship between Nutritional Status, Dental Caries, Mutans Streptococci, and Lactobacillus Bacterial Colonies

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          Abstract

          The prevalence of dental caries and obesity is high as both raise significant health problems. The objective of this study was to evaluate the relationship between dental caries, the number of salivary colonies forming units of Mutans Streptococci (MS) and Lactobacillus (LB), and the nutritional status in a group of children from Transylvania. This observational study used a sample of 154 school children, aged 9 to 12 years. The prevalence of caries was measured using the decayed, missing, and filled teeth index for deciduous teeth (dmft index) and for permanent teeth (DMFT index). Height and weight were assessed for each subject, and their body mass index (BMI) percentile was calculated. Salivary levels of Mutans Streptococci (MS) and Lactobacillus (LB) were determined using the CRT Bacteria Test from Ivoclar Vivadent. In our study, we found a positive association between the BMI percentile, MS count, LB count, tooth brushing frequency, and the incidence of dental caries in children aged 9 to 12 years old. Future preventive programs should include nutrition control in order to prevent both the apparition of dental caries and obesity in children.

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          The Biology of Streptococcus mutans

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            A practical guide to the oral microbiome and its relation to health and disease.

            The oral microbiome is incredibly complex with the average adult harboring about 50-100 billion bacteria in the oral cavity, which represent about 200 predominant bacterial species. Collectively, there are approximately 700 predominant taxa of which less than one-third still have not yet been grown in vitro. Compared to other body sites, the oral microbiome is unique and readily accessible. There is extensive literature available describing the oral microbiome and discussing the roles that bacteria may play in oral health and disease. However, the purpose of this review is not to rehash these detailed studies but rather to educate the reader with understanding the essence of the oral microbiome, namely that there are abundant bacteria in numbers and types, that there are molecular methods to rapidly determine bacterial associations, that there is site specificity for colonization of the host, that there are specific associations with oral health and disease, that oral bacteria may serve as biomarkers for non-oral diseases, and that oral microbial profiles may have potential use to assess disease risk.
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              Is obesity an oral bacterial disease?

              The world-wide explosion of overweight people has been called an epidemic. The inflammatory nature of obesity is widely recognized. Could it really be an epidemic involving an infectious agent? In this climate of concern over the increasing prevalence of overweight conditions in our society, we focus on the possible role of oral bacteria as a potential direct contributor to obesity. To investigate this possibility, we measured salivary bacterial populations of overweight women. Saliva was collected from 313 women with a body mass index between 27 and 32, and bacterial populations were measured by DNA probe analysis. Levels in this group were compared with data from a population of 232 healthy individuals from periodontal disease studies. The median percentage difference of 7 of the 40 bacterial species measured was greater than 2% in the saliva of overweight women. Classification tree analysis of salivary microbiological composition revealed that 98.4% of the overweight women could be identified by the presence of a single bacterial species (Selenomonas noxia) at levels greater than 1.05% of the total salivary bacteria. Analysis of these data suggests that the composition of salivary bacteria changes in overweight women. It seems likely that these bacterial species could serve as biological indicators of a developing overweight condition. Of even greater interest, and the subject of future research, is the possibility that oral bacteria may participate in the pathology that leads to obesity.
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                Author and article information

                Contributors
                Role: Academic Editor
                Journal
                Int J Environ Res Public Health
                Int J Environ Res Public Health
                ijerph
                International Journal of Environmental Research and Public Health
                MDPI
                1661-7827
                1660-4601
                29 March 2021
                April 2021
                : 18
                : 7
                : 3551
                Affiliations
                [1 ]Faculty of Dental Medicine, University of Medicine and Pharmacy, Science and Technology George Emil Palade, 540139 Târgu-Mureș, Romania; Eugen.bud@ 123456umfst.ro (E.S.B.); cristina.bica@ 123456umfst.ro (C.I.B.); oana.stoica@ 123456umfst.ro (O.E.S.); Alexandru.vlasa@ 123456umfst.ro (A.V.); daniela.esian@ 123456umfst.ro (D.E.); manuela.chibelean@ 123456umfst.ro (M.C.); mariana.pacurar@ 123456umfst.ro (M.P.)
                [2 ]Faculty of Medicine, University Dimitrie Cantemir, 540545 Târgu-Mureș, Romania
                Author notes
                [* ]Correspondence: bucursoranamaria@ 123456gmail.com (S.-M.B.); anamaria.bud@ 123456umfst.ro (A.B.); Tel.: +40-744437661 (S.-M.B.); +40-742825920 (A.B.); Fax: +40-742825920 (A.B.)
                Author information
                https://orcid.org/0000-0001-5193-4944
                https://orcid.org/0000-0001-5433-1137
                Article
                ijerph-18-03551
                10.3390/ijerph18073551
                8038087
                33805541
                98675308-dc1c-4a38-ae61-20e4a577b5cc
                © 2021 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 10 February 2021
                : 26 March 2021
                Categories
                Article

                Public health
                bmi,dental caries,children,obesity,dmft
                Public health
                bmi, dental caries, children, obesity, dmft

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