Abstracts are listed in alphabetical order by the last name of the senior author.
Characterization of a novel peptide with allatotropic activity in the fall armyworm,
Spodoptera frugiperda
M. Abdel-latief, M. Meyering-Vos and K.H. Hoffmann
Department of Animal Ecology I, University of Bayreuth, 95440 Bayreuth, Germany
klaus.hoffmann@uni-bayreuth.de
The biosynthesis of juvenile hormone (JH) by the corpora allata (CA) can be either
stimulated or inhibited by neuropeptides termed allatotropin (AT) or allatostatin
(AS), respectively. To date, only one AT has been identified. It was first isolated
from heads of pharate adults of Manduca sexta (Manse-AT; GFKNVEMMTARGF-NH2). The gene
encoding Manse-AT has been cloned from various lepidopterans, including S. frugiperda.
Here we report the identification of a second peptide with allatotropic activity from
the brain of S. frugiperda by molecular techniques. A cDNA that encodes 53 amino acids
included one copy of the RVRG NPISCF-OH peptide. This peptide strongly stimulated
the synthesis and release of JH in vitro by the CA of female adult S. frugiperda and
was code-named Spofr-AT 2. The stimulation was dose-dependent with an app. EC50 of
ca. 10−7 M. CA that were activated with Spofr-AT 2 could be inhibited by the addition
of Manse-AS to the incubation medium. Northern blotting and RT-PCR analyses revealed
that the preprohormone is expressed in the brain, midgut, and ovary in a developmental-specific
manner. Whole-mount in situ hybridisation confirmed the gene expression in various
tissues of adult females. Supported by the DFG (Ho 631/15-4).
Juvenile hormone accelerates ovarian development and does not affect age polyethism
in the primitively eusocial wasp, Ropalidia marginata
M. Agrahari1 and R. Gadagkar1,2
1Indian Institute of Science, Centre for Ecological Sciences, Bangalore 560 012, India
2Jawaharlal Nehru Centre for Advanced Scientific Research, Evolutionary and Organismal
Biology Unit, Jakkur, Bangalore 560 064, India
mauli@ces.iisc.ernet.in
Juvenile hormone modulates post-imaginal reproductive division of labor in primitively
eusocial species and promotes the production of queens (e.g., Polistes) while it modulates
age polyethism and promotes the production of foragers in highly eusocial species
(e.g., the honey bee). Ropalidia marginata is a primitively eusocial wasp that shows
both post-imaginal regulation of reproductive division of labor as well as age polyethism.
Hence, R. marginata is a particularly interesting model system to study the effect
of juvenile hormone. We demonstrate here that a single, topical application of 100
µg of juvenile hormone-III per female wasp accelerates ovarian development of wasps
held in isolation. Similar application to wasps released back on to their natal nests
has no effect on their rate of behavioral development as witnessed from the age of
first performance of feed larva, build, bring pulp and bring food. We conclude therefore
that in R. marginata, juvenile hormone has retained its function of modulating reproductive
division of labor and has not acquired the function of modulating age polyethism.
Uncovering Juvenile Hormone's Mode of Action in Ips pini (Say)
J.C. Bearfield, S. Young, G. J. Blomquist and C. Tittiger
Department of Biochemistry, University of Nevada, Reno, NV 89557-0014, USA
jeremyb@unr.nevada.edu
In the pine engraver beetle, Ips pini, pheromone components are produced de novo via
the mevalonate pathway. Feeding and JH III treatment induce an increase in pheromone
biosynthetic gene expression in male midgut cells, especially 3-hydroxy-3-methylglutaryl-CoA
reductase (HMG-R) and synthase (HMG-S). Microarray analysis has identified several
other mevalonate pathway genes, as well as a few unknowns, that appear to be JH-responsive.
Further expression analyses using real-time and semi-quantitative RT-PCR prepared
from midguts at various time points (within the first 8 hours) after JH treatment
have revealed some that are rapid, primary JHresponders, with transcripts visible
after only 1 hour. The promoter regions of JH-responsive genes are needed to search
for putative JH response elements. Therefore, an I. pini genomic library was screened
using JH-responsive clones in order to isolate their 5′ flanking regions. Electrophoretic
mobility shift assays using nuclear extracts prepared from Sf21 cells treated with
either hormone or carrier alone were performed to identify regions of JH-induced protein
binding. Also, important regions for induction are being mapped using transcriptional
assays with luciferase reporter constructs. The isolation of JH-inducible genes and
their regulatory sequences will provide vital information about the mechanism of JH
action in the Coleoptera.
Unique structural features of moth farnesyl diphosphate synthase: implications for
the biosynthesis of homologous juvenile hormones
C. Béliveau1, S. Sen2, P. Rehse3 and M. Cusson1
1Laurentian Forestry Centre, NRCan-CFS, 1055 du PEPS, Sainte-Foy, QC, G1V 4C7, Canada
2Dept. of Chemistry, Indiana University-Purdue Universit, Indianapolis, Indiana, 46202,
USA
3RIKEN Harima Institute, Mikazuki, Sayo, Hyogo, 679-5148, Japan
cusson@cfl.forestry.ca
Although the vast majority of insects produce only one form of juvenile hormone (JH
III), the Lepidoptera produce a total of five JHs, including four structural homologs
that feature one or more ethyl side chains. In a key step of JH biosynthesis, three
isoprene units undergo a head-totail condensation catalysed by farnesyl diphosphate
synthase (FPPS), generating the C15 farnesyl diphosphate – the backbone of JH – or
one of its C16-18 structural homologs. We cloned and sequenced FPPS cDNAs from two
species of moths. A comparison of these sequences with those of all other known FPPSs
– cloned from various organisms ranging from Drosophila melanogaster to Homo sapiens
– revealed several amino acid substitutions within the catalytic cavity. Molecular
modeling of moth FPPS suggests that some of these substitutions could provide the
extra space needed for binding the bulkier ethyl-substituted substrates/products.
In assays aimed at assessing the substrate specificity of the recombinant enzymes,
we observed that moth FPPS displays a relative activity twice as high as that of Drosophila's
in the presence of homogeranyl-PP and [3H]-isopentenyl-PP, suggesting that the moth
enzyme shows preference for homologous substrates
Juvenile hormone regulation of bark beetle monoterpenoid pheromone biosynthesis
G.J. Blomquist, C.I. Keeling, A. Gilg-Young, J. Bearfield, M.D. Ginzel, S. Young and
C. Tittiger
Department of Biochemistry, University of Nevada, Reno, NV, 89557-0014, USA
garyb@cabnr.unr.edu
Juvenile hormone (JH) III regulates de novo aggregation pheromone production in bark
beetles. Feeding on host phloem or application of JH III induces the production of
ipsdienol in male Ips pini and frontalin in male Dendroctonus jeffreyi in a dose and
time related manner. The incorporation of [14C]mevalonolactone into ipdienol and frontalin
demonstrated that these pheromone components were produced via the mevalonate pathway.
JH III induction of 3hydroxy-3-methylglutaryl-CoA reductase, a key regulatory enzyme
in the mevalonate pathway, was localized to midgut tissue by in situ hybridization.
Studies with [14C]acetate demonstrated that midgut tissue from male I. pini produced
ipsdienol and from male D. jeffreyi produced frontalin. This work led to a paradigm
shift in which it is now recognized that most, but not all, monoterpenoid pheromone
components from bark beetles are produced de novo. It also provided the background
to current studies using microarrays to examine JH III regulation of mevalonate pathway
enzymes in midgut tissue, the characterization and JH III regulation of geranyl diphosphate
synthase, the discovery of putative JH III regulatory elements and a novel regulatory
motif in I. confuses.
The effect of juvenile hormone on the PBAN-receptor and pheromone production in the
moth Helicoverpa armigera: An aging hormone in adult females?
R. Bober, T. Zacharova and A. Rafaeli
Institute for Technology & Storage of Agricultural Products, ARO, Volcani Center,
P.O. Box 6, Bet Dagan, Israel
vtada@volcani.agri.gov.il
The present study was designed to elucidate the effect of Juvenile Hormone (JH) on
PBAN activation of pheromone production in vitro and on specific binding to a PBAN-receptor
(PBANR) in pheromone glands of H. armigera females. PBAN can activate pheromone biosynthesis
only in pheromone glands of newly emerged adult females, after which levels increase
with age and reach maximum levels in 3 and 4 day old female glands, thereafter the
pheromonotropic response decreases. Female pharate pupae do not respond to PBAN but,
in the presence of JH II or its analog, fenoxycarb (FX), PBAN can induce a response
in pharate females. In addition, a photoaffinity-biotin-labeled PBAN-analog, specifically
binds to a 50 kD putative PBAN membrane receptor in adult females. This binding is
absent in pharate females but can be induced after JH II or FX treatments to levels
of newly emerged females, thereby providing evidence that JH up-regulates the PBAN-R
in immature females. In contrast, in 3–4 day-old mature adult females, FX causes down-regulation
of both pheromone production and PBAN-R binding to levels present in older females.
Taken together these data indicate that juvenile hormone treatment shifts the normal
age-dependent response to PBAN.
Effect of Precocene II on Fatty Acid Metabolism in the Pea Aphid, Acyrthosiphon pisum
Under Cold Stress
Zhaorigetu Chen1, Robin D. Madden2, & Jack W. Dillwith2.
1Section of Integrative Biology, University of Texas at Austin, TX 78712
2Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater,
OK, 74078, USA
zc239@mail.utexas.edu
Pea aphid, Acyrthosiphon pisum, accumulate high levels of myristic acid as triglycerides
at 10°C. To investigate whether juvenile hormone (JH) mediates fatty acid metabolism
in the aphids, the aphids from 25°C colony were treated with precocene II (a JH antagonist)
at 0.5 and 2 µg/aphid, respectively, then reared at 10°C for 16 days. An increase
in fatty acid content was observed in the aphids treated with 0.5 µg, whereas no change
was observed in 2 µg treatment. When the aphids were retreated with same amount of
precocene II 24 h after 1st treatment, the fatty acid content in the aphids did not
change in either treatment. A further experiment was conducted to investigate the
reproductive fecundity in the aphids treated with 2 µg precocene II. The nymphs per
aphid in the treated aphids decreased significantly at either 25°C or 10°C compared
to that in the control aphids. However, the nymphs per day in treated aphids at 10°C
is similar to that in the control aphids. These results imply that JH may mediate
fatty acid metabolism and affect the reproduction in the pea aphids reared at low
temperatures.
Molecular characterization of the lipophorin receptor of Blattella germanica
L. Ciudad, M.D. Piulachs and X. Belles
Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular
de Barcelona (CSIC), Jordi Girona 18, 08034 Barcelona, Spain
xbragr@ibmb.csic.es
We have cloned and molecularly characterized a Blattella germanica lipophorin receptor
(BgLpR) cDNA. It has a length of 2595 bp encoding a 865-residue protein with a predicted
molecular mass of 96.8 kDa. BgLpR is a member of the low density lipoprotein receptor
family with five domains: ligand domain, epidermal growth factor precursor, putative
O-linked sugar, transmembrane domains, and cytoplasmic tail with the internalization
signal FDNPVY. In adult females, BgLpR is expressed in the fat body and the ovary
during the 7 days of the first gonadotrophic cycle. In the ovary, mRNA levels are
high during the first 5 days and suddenly decrease on days 6 and 7. In the fat body,
mRNA levels are high at adult emergence, decrease on days 1 and 2, increase from day
3 to day 5, and slightly decrease on days 6 and 7. The pattern in the ovary corresponds
to the dynamics of lipophorin incorporation into the ooplasm during the vitellogenic
cycle. That in the fat body may be related with a lipid scavenger activity. The pattern
difference in the fat body and in the ovary suggests that the regulatory mechanisms
in these two organs are different. The possible role of JH in BgLpR regulation is
discussed.
Expression and localization of a putative farnesoic acid o-methyltransferase (FAMeT)
in Drosophila melanogaster
L. Dayton1, S.S. Tobe2 and W.G. Bendena1
1 Department of Biology, Queen's University, Kingston, Ontario, K7L 3N6, Canada
2 Department of Zoology, University of Toronto, Toronto, Ontario, M5S 3G5, Canada
daytonl@biology.queensu.ca
Farnesoic acid o-methyl transferase (FAMeT) is an important enzyme in the pathway
leading to juvenile hormone biosynthesis. Juvenile hormone participates in the development
and reproduction of most insects. Database searches based on sequence identity with
crustacean FAMeT has revealed a putative gene product in Drosophila melanogaster.
This gene encodes a protein with 41% sequence identity to Metapenaeus ensis. It is
thought that FAMeT may play a rate limiting role in juvenile hormone biosynthesis
in D. melanogaster; however, no experimental evidence has been reported. In order
to elucidate the putative Drosophila FAMeT orthologue's role in juvenile hormone biosynthesis,
we have investigated protein distribution, activity and in vivo transcription in D.
melanogaster. This has been examined by 1) protein expression to test FAMeT's activity
in vitro and 2) by fusion of the putative FAMeT gene promoter to green fluorescent
protein (GFP) within a p-element vector. Germ-line transformations of this construct
will be carried out to provide localization data.
Up-regulation of JH levels in Lacanobia oleracea larvae attacked by the parasitoid
wasp Eulophus pennicornis, is associated with reduction in host JH esterase activity
J.P. Edwards1, H.A. Bell1, G.C. Marris1, A. Kirkbride-Smith1, G. Bryning1 and M. Cusson2
1Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK
2Laurentian Forestry Centre, Sainte-Foy, Quebec, G1V 4C7, Canada
j.edwards@csl.gov.uk
The ectoparasitoid wasp Eulophus pennicornis attacks 5 th and 6th instar larvae of
its host Lacanobia oleracea. We measured the activity of juvenile hormone esterase
(JHE) daily in unparasitized and parasitized penultimate (5th) and final (6th) instar
host larvae. In unparasitized larvae, JHE activity was low throughout the penultimate
larval stadium, but was markedly higher in final instar larvae. In parasitized penultimate
instar larvae, JHE levels were similar to those in unparasitized insects. However,
in parasitized final instar larvae, JHE activity was markedly reduced when compared
to the levels in unparasitized larvae. In the light of these differences, we measured
the titres of juvenile hormones in parasitized and unparasitized larvae, on days 2
and 5 of the final larval stadium. Whereas JH levels were virtually absent in unparasitized
larvae, substantial quantities of JH (∼100-fold higher) were detected in parasitized
larvae of the equivalent ages. We have also investigated the JH present in larval
and adult stages of the parasitoid. In both parasitoid larvae and in adult wasps,
we found high titres of JH III, but little JH II, and no JH I.
The making of a yolky egg: It takes more than juvenile hormone and reserves
F. Engelmann
Department OBEE, University of California, Los Angeles, CA 90095, USA
fengelma@ucla.edu
In the majority of insects the production of a yolky egg necessitates the induction
of vitellogenin (Vg) by juvenile hormone (JH). Vg, i.e. vitellin (Vt), is the predominent
yolk protein. Also, lipophorin (Lp) and hexamerins, contribute quantitatively to the
protein yolk of the egg. In cockroaches, normally only JH drives the de novo synthesis
of Vg. Adequate titers of JH are maintained through the controls of rates of JH synthesis
and JH degradation. Using the example of the cockroach Leucophaea maderae the complexity
of controls that allow a timely making of a mature egg will be discussed. This includes
the determinations of 1) the JH and Vg profiles, 2) the JH controlled augmentation
of lipophorin (Lp) synthesis, 3) the JH influenced increase in JH esterase titers
during vitellogenesis and 4) the assessement of the involvement of the hexamerins.
Lp and Vg are the 2 major JH binding proteins in circulation. Consequently, the rate
of degradation of JH is efficiently reduced even during periods when we measure the
highest titers of the JH esterases. Conversely, in the absence of Lp the circulating
JH is short lived and therefore Vg production is reduced resulting in a curtailed
egg production.
The role of JH and its effector, Broad in the evolution of insect metamorphosis
D.F. Erezyilmaz, L.M. Riddiford and J.W. Truman
Department of Biology, University of Washington, Seattle, WA, 98195, USA
denizere@u.washington.edu
Metamorphosis in Holometabola is regulated by JH and its effector, Broad. To begin
to understand how complete metamorphosis has evolved in insects, we examined the roles
of JH and Broad in hemimetabolous insects. We have found, by simulating the JH profile
of holometabolous embryos in cricket embryos, that JH is able to redirect embryonic
cuticle formation in a stage and dose-dependent way. To determine the role of Broad
in hemimetabolous insects, we cloned this gene from crickets and bugs. Unlike its
expression in Holometabola, where it is expressed exclusively during pupal development,
we found that the Z1 isoform of Broad is expressed during embryonic development of
crickets, first appearing during segmentation. We found that dsRNA knock down of Broad
resulted in embryos with posterior truncations. Another difference between hemimetabolous
and metamorphosing insects is seen during post-embryonic development. Our preliminary
data indicates that Broad is expressed during the nymphal stages, coincident with
high JH levels. Taken together, our data suggests that metamorphosis in insects could
have arisen after embryonic and nymphal expression of Broad acquired inhibition by
JH.
The regulation of vitellogenesis in the lubber grasshopper by JH and nutrition
H. Fei1, M. E. Wier2, K. M. Rhoads2, and D. W. Borst1
1Illinois State University, Normal, IL 61790
2Illinois Wesleyan University, Bloomington, IL, 61701
hfei@ilstu.edu
Hemolymph levels of vitellogenin (Vg) and juvenile hormone (JH) in the lubber grasshopper
(Romalea microptera) change in parallel during the oviposition cycle, suggesting that
JH may closely regulate Vg production. Likewise, the duration of the oviposition cycle
is regulated by the amount of food available. We investigated the relationships between
nutrition, JH, and vitellogenesis by feeding animals high (H; 4.5g lettuce and 0.6g
oats/d) and low (L; 1.5g lettuce and 0.2g oats/d) diets. JH levels in H-diet animals
increased on ∼ d10, reached a peak on d20-25, and declined thereafter. The levels
of Vg-mRNA in these animals show a similar rise and fall, and ovarian mass increased
30-fold. JH levels in L-diet animals remained low through d30, and Vg-mRNA levels
and ovarian mass rose only 4-fold above initial levels. When animals were switched
from the L-diet to the H-diet on d20, JH levels and Vg-mRNA levels rose quickly on
d25 and reached a maximum on d30. Levels of Vg-mRNA also rose about 9 and 10-fold
above initial levels on d25 and 30, respectively, and ovarian mass increased 11-fold
above initial levels. Infusion of L-diet animals with physiological levels of JH for
5 days stimulated Vg-mRNA levels, but the increase was intermediate to that of animals
switched to the H-diet over this period. These data suggest that nutrition regulates
vitellogenesis is complex, and only partly reflects its effects on JH levels. (Supported
by NSF grant DBI - 9978810 to DWB).
Juvenile Hormone Binding Proteins and Neuronal Plasticity
Y. Gaubard1, C. Gadenne2, G.D. Prestwich3,4, T. Shiotsuki5, C. Löfstedt1 and J-F.
Picimbon1,3
1Lund University, Department of Ecology, Sölvegatan 37, SE-223 62 Lund, Sweden
2INRA, Unité Mixte de Recherches en Santé Végétale, BP 81, 33883 Villenave d'Ornon
cédex, France
3Department of Chemistry, State University of New York, Stony Brook, New York 11794,
USA
4Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah 84112-5820,
USA
5National Institute of Agrobiological Sciences, 1–2 Owashi, Tsukuba, Ibaraki 305-8634,
JAPAN
yohann.gaubard@ekol.lu.se
In the black cutworm moth, Agrotis ipsilon, Juvenile Hormone (JH) has been shown to
control both pheromone production and perception. In particular, most recent studies
have shown the ability of males to adapt their pheromone behavior by modulation of
neural responses to the olfactory stimuli. This phenomenon may be directly related
to the regulation of pheromone sensitivity of interneurons in the antennal lobe. This
suggests that, in males, JH may act by controlling neuronal plasticity in the macroglomerular
complex. We aim at the molecular mechanism underlying JH regulation of neuroplasticity
associated to pheromone perception. With this respect, we have identified Juvenile
Hormone binding Proteins (JHBPs) in the brain of A. ipsilon. In photolabeling experiments,
the tritiated JH I analogue ([3H]-EBDA) bound to a protein with a molecular weight
of 30-35 kDa in protein extracts from A. ipsilon brains. Complementary DNA encoding
cytosolic JHBP was cloned from a brain cDNA library. This suggests the action of JH
at the intracellular level in brain cells. In all, we have identified the first JH-Binding
Protein from a moth brain and discuss its role in the control of neuroplasticity and
pheromone perception.
Selective Inhibition of Juvenile Hormone Biosynthesis by Ammonium Diphosphate Compounds
D. Gernert, R. Denton, K. Burns, and S. Sen
Department of Chemistry, Indiana U.-Purdue U. Indianapolis, 402 North Blackford Street,
Indianapolis, IN 46202, USA
sen@chem.iupui.edu
Juvenile hormone is biosynthesized by an unusual metabolic pathway that utilizes enzymes
of the MVA pathway. Two enzymes, FPP synthase and IPP isomerase are potential sites
for inhibitor development, particularly in light of the fact that they play a pivotal
role in the construction of JH homologs in Lepidoptera. A series of ammonium diphosphate
analogs of dimethylallyl diphosphate (DMAPP) and geranyl diphosphate (GPP) were prepared
and tested for their ability to inhibit JH biosynthesis in vitro, using corpora allata
homogenates of larval Manduca sexta. When potencies were compared to those obtained
with the corresponding porcine enzymes, differences were seen, indicating that similar
design strategies could be useful for the development of selective anti-juvenile hormone
agents.
The Isolation and Characterization of the First Animal Geranyl Diphosphate Synthase
from the Pine Engraver, Ips pini (Say)
A. Gilg-Young, C. Tittiger, W. Welch and G.J. Blomquist
Department of Biochemistry, University of Nevada, Reno, NV 89557-0014, USA
annagy@unr.edu
Bark beetles release aggregation pheromones that coordinate the colonization of their
coniferous hosts. Over the years, debate has ensued over whether these monoterpene
pheromone components are derived from dietary precursors or produced de novo. We present
evidence in support of their de novo biosynthesis via the mevalonate pathway. We have
isolated the first animal geranyl diphosphate synthase (GPPS) from a cDNA library
of juvenile hormone (JH) III treated male Ips pini. GPPS catalyzes the formation of
the C10 precursor of ipsdienol, a major aggregation pheromone component in male I.
pini. We have expressed the recombinant GPPS and assayed for functional activity.
Sequence comparisons and structural modeling show conserved motifs and an all ?-helical
fold similar to other short-chain isoprenyl diphosphate synthases. In a number of
bark beetles, JHIII regulates pheromone production. Northern analysis of I. pini shows
GPPS transcript levels are up-regulated in a JHIII dose-and time dependent manner,
similar to other mevalonate genes involved in aggregation pheromone biosynthesis in
male I. pini.
The role of juvenile hormone in the endocrine regulation of pheromone production in
the pinyon Ips
M.D. Ginzel, C.I. Keeling, C. Tittiger and G.J. Blomquist
Department of Biochemistry, University of Nevada, Reno, NV 89557-0014, USA
ginzel@uiuc.edu
Bark beetles are among the most economically important forest pests in the northern
hemisphere and rely on monoterpenoid aggregation pheromones to coordinate host colonization
and mating. In this study, we investigate the interplay between feeding on host phloem
and the induction of de novo pheromone biosynthesis in Ips confusus, the pinyon Ips.
I. confusus has become a major pest in the southwestern United States, destroying
hundreds of thousands of acres of pinyon pines. Juvenile hormone (JH) III regulates
pheromone production in a number of bark beetles. Interestingly, it appears that JH
III alone does not stimulate pheromone biosynthesis in male I. confusus but rather
some other regulatory factor, perhaps a brain hormone, is required for pheromone production.
We have found that feeding on host phloem, but not JH III treatment, strongly induces
pheromone production in male I. confusus. Moreover, feeding alone stimulates the activity
of a key mevalonate pathway enzyme, 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMG-R),
only in males. Nevertheless, feeding and JH III both significantly up-regulate mRNA
levels of HMG-R and other mevalonate pathway genes.
Presence and possible function of allatotropin in developmental stages of Heliothis
virescens and Apis mellifera
J. M. Glasscock and A. Rachinsky
Department of Biology, University of Minnesota Duluth, Duluth, MN, 55812, USA
arachins@d.umn.edu
Juvenile hormone biosynthesis is, in part, regulated by stimulatory neuropeptides
called allatotropins. Using immunocytochemical techniques, we localized Manduca sexta
allatotropincontaining cells in the central nervous system (CNS) of selected developmental
stages of two insect species, the tobacco budworm Heliothis virescens and the honeybee
Apis mellifera. H. virescens produces allatotropin (AT) consistently throughout larval
development. The distribution patterns of AT-containing cells in the CNS persisted
from one larval instar to the next, and were similar to the distribution patterns
in adults. However, the total number of AT-containing cells in the brain gradually
increased during larval development. In the honeybee A. mellifera, AT-containing cells
were only found in a few brains from late last instar larvae (prepupae). AT was present
in a group of 6–8 cells in the pars intercerebralis. However, we did not find any
AT in brains of early last instar larvae, whose corpora allata (CA) were shown to
be more sensitive to in vitro stimulation by AT than prepupal CA. The significance
of our results in the context of insect development in general, and in the context
of caste development in honeybees will be discussed.
Juvenile hormone in the control of reproductive function in Drosophila virilis under
stress
N.E. Gruntenko1, M. Bownes2, N.V. Adonyeva1, J. Terashima2, E.K. Karpova1 and I.Yu.
Rauschenbach1
1Institute of Cytology and Genetics SD RAS, Novosibirsk 630090, Russia
2Institute of Cell and Molecular Biology, University of Edinburgh, The King's Buildings,
Edinburgh EH9 3R, UK
nataly@bionet.nsc.ru
To unravel the role of juvenile hormone (JH) in the control of reproduction under
stress, JH degradation and reproduction were studied under heat and nutritional stresses
and JH treatment in wild type (wt) Drosophila virilis females and in a heat stress
(hs) mutant. The hs mutation prevents alterations in JH metabolism under heat stress.
Both heat stress and starvation result in a decrease of JH degradation, a delay in
oocyte maturation, degradation of early vitellogenic egg chambers, accumulation of
mature oocytes, an oviposition arrest and followed by decrease in fertility in wt
females. JH treatment leads to a decrease in JH degradation and an arrest of oviposition
for 24h in wt and hs females. JH treatment prior starvation seems to protect some
oocytes from degradation: in JH-treated wt female's fertility increases rapidly following
the end of starvation. The dynamics of JH degradation and fertility are similar following
stress and JH treatment in wt females. In hs females, there is no decrease in JH degradation,
accumulation of mature oocytes and oviposition arrest under heat stress. However,
they show all these alterations under starvation. This suggests that JH controls later
stages of oogenesis and oviposition under stress.
Is methyl palmitate an indicator of reproductive maturation in female Medflies?
T. Herman1, I. Miloslavski2, Z. Aizenshtat2 and S.W. Applebaum1
1Department of Entomology, The Hebrew University of Jerusalem, Rehovot Campus, Israel
2Department of Organic Chemistry, The Hebrew University of Jerusalem, Givat- Ram Campus,
Israel
herman@agri.huji.ac.il
Methyl palmitate (MP) is a biosynthetic in vitro product of the corpus allatum (CA)
of adult female (Ceratitis capitata; Diptera:Trypetidae). The presence of MP in whole-body
extracts of virgin females has now been determined by GC/MS. Methyl esters peaks were
identified by Total Ion Current chromatography and MP quantified by Single Ion monitoring.
In mated Drosophila melanogaster, reproductive maturation is primed via enhanced allatal
production of JHB3, induced by the N-terminus of the male-derived Sex Peptide (DrmSP).
We hypothesize that the CA of young female Medflies are deficient in either epoxidase,
or in farnesoic acid, the sesquiterpene precursor of JH-biosynthesis, and that biosynthesis
is therefore diverted via acetyl–CoA carboxylase to the alternative production of
palmitic acid (C16:0; PA). During this transition period, the CA methylates PA to
MP, which by inference presupposes the existence of endogenous methyl transferase.
Accordingly, mating is assumed to enhance the acquisition of enzymes/substrates for
production of JH-III and JHB3, the conventional products of mature dipteran CA.
The significance of allatal reproductive maturation: Multiple functions of insect
allatotropins
F.M. Horodyski1, K.-Y. Lee2 and M.E. Chamberlin3
Departments of Biomedical Sciences1 and Biological Sciences3, Ohio University, Athens,
OH, 45701, USA
2Department of Agricultural Biology, Kyungpook National University, Taegu, Korea
horodysk@ohio.edu
Juvenile hormone (JH) levels in the hemolymph are regulated in part by the actions
of neuropeptides that act on the corpora allata (CA). An allatotropin (Manse-AT) was
isolated from Manduca sexta that stimulates JH biosynthesis by the adult female CA
in vitro, and similar peptides have been isolated or predicted from several insect
species that exhibit a variety of additional roles on muscles, heart and midgut. In
other invertebrate orders, Manse-AT-related peptides exhibit myotropic functions suggesting
that this might be the ancestral role for this family of peptides. Tissue- and stage-specific
alternative splicing predicts the synthesis of allatotropin-like (ATL) peptides that
possess overlapping biological activities in M. sexta. The levels of one of the alternatively
spliced Manse-AT mRNAs is elevated in larvae that were starved, parasitized, or fed
the ecdysteroid agonist RH-5992. These elevated mRNA levels were seen exclusively
in the terminal abdominal ganglion of starved larvae. Although the CA of mated M.
sexta females exhibit an elevated rate of JH biosynthesis in vitro, levels of Manse-AT
mRNAs were similar to those in virgin females, suggesting that other factors might
be involved in the elevated CA activity.
JH titers, biosynthesis and metabolism in honey bee workers infected by a microsporidia
parasite, Nosema apis
Z.Y. Huang and R. Lin
Department of Entomology, Michigan State University, East Lansing, MI 48824, USA
bees@msu.edu
It is known that Nosema apis (a microsporadian parasite) accelerates the behavioural
development in honeybee workers, but the underlying mechanisms are unknown. Early
studies suggested that Nosema may be capable of producing juvenile hormone (JH) directly.
We studied the JH production and metabolism of Nosema infected workers. Infected workers
foraged at an earlier age and showed higher haemolymph JH titers than control bees.
This suggests that Nosema infection induces workers to forage earlier via higher JH
titers. Nosema infected workers showed higher rates of JH biosynthesis than control
bees when workers were 6–8 days old (preforaging). Allatectomized workers fed Nosema
had no detectable levels of juvenile hormone in hemolymph. The majority of these workers
did not show earlier foraging compared to the control group (allatectomized bees with
no Nosema). Finally, Nosema-infected workers also showed higher JH degradation compared
to control bees. These results suggest that Nosemainfected workers forage at an earlier
age than control bees due to higher JH titers, which was due to increased JH production,
and despite of the increased JH degradation in infected bees. Our results also suggest
that Nosema apis does not produce JH directly, but can induce the host CA to produce
higher titers of JH. Earlier foraging of the hosts might be advantageous for the parasite
because precious foragers may drift more easily to other colonies.
Transcriptional Integration of JH and Ecdysone Signaling Through Nuclear Receptor
Dimer Containing Ultraspiracle
D. Jones1, G. Jones2, Y. Xu2, Y.-X. Chu2, F. Fang1 and R. Thomans2
Graduate Center for Toxicology1 and Dept. of Biology2, University of Kentucky, Lexington,
KY, 40506, USA
djones@uky.edu
Using intrinsic fluorescence and anisotropy techniques, we have demonstrated binding
of methyl epoxyfarnesoate (juvenile hormone (JH) III) to USP. In an Sf9 cell transfection,
JH IIIactivation pathway, JH III can act through the USP ligand binding pocket and
through a heterologous USP binding site (direct repeat motif) to activate the reporter
core promoter. Additional, exogenous USP further increases the loading of USP onto
the direct repeat binding site (by gel mobility shift assay) and increases transcriptional
activation by treatment with JH III alone. Exogenous ecdysone receptor (EcR) increases
the loading of the USP/EcR dimer onto the direct repeat binding site and increases
activation due to treatment with 20-OH ecdysone. Treatment with both JH III and 20-OH
ecdysone activates through the direct repeat binding site to a much greater than the
additive effect of either hormone alone, and exogenous EcR also enhances this synergism,
indicating synergism of the two hormones is mediated by the USP/EcR dimer. However,
exogenous EcR suppresses activation by JH III alone, indicating that JH III activation
through USP is subordinated by the USP/EcR heterodimer when the EcR partner is unliganded.
Transcriptional Transduction of Juvenile Hormone Signaling by the Nuclear Hormone
Receptor Ultraspiracle
G, Jones1, D. Jones2, Y. Xu1, Y.-X. Chu1 and F. Fang2
Department of Biology1 and Graduate Center for Toxicology2, University of Kentucky,
Lexington, KY, 40506, USA
gjones@uky.edu
The published crystal structure of the nuclear receptor Ultraspiracle (USP) contributed
to a paradigm of USP as a ligandless receptor, in which the AF2 of USP is maintained
in an antagonist position over the site corresponding to a coactivator binding surface.
Using intrinsic fluorescence and anisotropy techniques, we have demonstrated binding
of methyl epoxyfarnesoate (juvenile hormone (JH) III) to USP. The interaction of JH
I and JH III with the USP ligand binding pocket is qualitatively different than that
of JH II. The binding by JH III promotes receptor oligomerization and repositioning
of AF2. In a cell transfection, JH IIIactivation pathway, JH III can act through a
heterologous USP binding site (DR12 motif) to activate the reporter core promoter.
Specific point mutations to particular ligand binding pocket residues alter or prevent
JH III binding, and render the mutant receptor to be a dominant negative in this system.
The mutant USP with reduced JH III binding and dominant negative action in the cell
transfection, JH III-activation pathway is reduced in its ability as a transgene to
replace missing function of the wild type USP in null USP flies.
Evaluation of the vinyl sulfoxide class of anti-juvenoids as inhibitors of isoprenoid
forming enzymes
S. Jull, G. Ewing, and S. Sen
Department of Chemistry, Indiana University & Purdue University Indianapolis, 402
North Blackford Street, Indianapolis, IN 46202, USA
sen@chem.iupui.edu
Several vinyl sulfoxides are known to possess topical anti-juvenile hormone activity.
While these compounds inhibit one or more steps prior to farnesoic acid formation,
their specific mode of action is unknown. We hypothesized that the most active compound
(1) could function as a transition state analog for the isoprenoid forming enzymes,
FPP synthase and IPP isomerase, being a mimic of the carbocations that form during
catalysis. To test this hypothesis, derivatives of 1 were prepared, including several
potential metabolites that could form upon topical application. The compounds were
tested for in vitro activity using corpora allata homogenates of Manduca sexta. None
of the compounds were inhibitory, with the exception of the diphosphate analog of
1, which was a modest inhibitor of FPP synthase. These results suggest that metabolic
activation of 1, through hydrolysis and subsequent phosphorylation via endogenous
kinases, may be operational.
Anti-metamorphic effect of an endoparasitoid, Cotesia plutellae, on Plutella xylostella
– Is it caused by alteration of JH titer or ecdysteroidogenesis?
S. Lee and Y. Kim
School of Bioresource Sciences, Andong National University, Andong 760-749, Korea
hosanna@andong.ac.kr
An endoparasitoid wasp, Cotesia plutellae, parasitized the diamondback moth, Plutella
xylostella, and inhibited larva-pupal metamorphosis. JH esterase (JHE) in the hemolymph
of P. xylostella was monitored by use of a surrogate substrate, HEPTAT (methyl 1heptylthioacetothioate).
The enzyme kinetic parameters of the fourth instar larvae of the nonparasitized showed
that Km was 19.70 µM and Vmax was 228.83 µM/min/µl. In the last larval instar of P.
xylostella, JHE activity kept high level, but decreased before pupation. Similarly,
the parasitized larval showed high level of JHE activity in the initial final instar,
which was not significantly different to that of the non-parasitized. Thus, the parasitization
by C. plutellae did not inhibit host JHE. In comparison, the prothoracic gland showed
significant morphological changes between parasitized and non-parasitized larval,
even though the application of an ecdystroid agonist, RH5992, did not induce metamorphosis
of the parasitized larvae.
Juvenile hormone-mediated gene expression in the midgut of the pine engraver beetle,
Ips pini (Say)
C.I. Keeling, S. Young, and C. Tittiger
Department of Biochemistry/MS 330, University of Nevada, Reno, NV, 89557-0014
ckeeling@mac.com
The pine engraver beetle is a significant pest of North American coniferous forests.
The male is the pioneering sex and attracts mates to its nuptial chamber in the phloem
of a host tree with a pheromone. Upon feeding, and regulated by juvenile hormone III
(JH), the pheromone component ipsdienol is biosynthesized de novo via the mevalonate
pathway in the male midgut. Identifying and characterizing genes in the midgut that
respond to JH treatment may yield the mode of JH action as well as targets for novel
pest management strategies. To identify responsive genes, cDNA microarrays were prepared
to represent the unique genes previously identified in a midgut EST project and then
hybridized with fluorescently labeled cDNA from midgut tissue from JH-treated and
control beetles of both sexes over a time course. Several genes were significantly
up or down regulated by JH, including known mevalonate genes, other known genes, and
unknown genes. The expression of these genes was further examined by quantitative
real-time RT-PCR. Transcript levels in the midgut of both sexes respond to JH although
there was a sex-specificity consistent with pheromone biosynthesis.
Juvenile hormone action involves multiple signal transduction mechanisms
D.R. Kethidi and S.R. Palli
Department of Entomology, University of Kentucky, Lexington KY 40545, USA
rpalli@uky.edu
Although, the biological actions of juvenile hormones (JH) in regulating development
and reproduction are well studied, the molecular basis of JH action is poorly understood.
We discovered that the juvenile hormone esterase gene from Choristoneura fumiferana
(Cfjhe) is induced by JH I and JH I induction is suppressed by 20-hydroxyecdysone
(20E). Cloning and analysis of the promoter region of Cfjhe gene identified a 30 bp
region that supports both JH and 20E response observed for the Cfjhe gene. This 30
bp sequence contains a direct repeat element with a 4-nucleotide spacer and is designated
as JH response element (JHRE). A reporter gene placed under the control of JHRE is
induced by JH in a dose- and time-dependent manner in CF-203, L57, HvE1s and Aa23
cells, and 20E in the presence of EcR was able to suppress JH induction. Nuclear proteins
isolated from all four cell lines bound to JHRE. Protein kinase C-mediated phosphorylation
decreased the binding of nuclear proteins to JHRE. JH or calf intestinal alkaline
phosphatase-mediated dephosphorylation increased the binding of nuclear proteins to
JHRE. The results from these studies suggest that JH action and its cross-talk with
20E involve multiple signal transduction mechanisms.
Methyl Farnesoate Controls Adult Male Morphogenesis in a Crayfish
H. Laufer, N. Demir and J.S.B. Ahl
Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 06269-3125
Laufer@uconn.edu
Insects mature sexually after a terminal molt. Louisiana crayfish, Procambarus clarkii,
continue to molt as adults, switching between Form Is, primary reproductives, with
large chelae and spines on ischiopodites of walking legs 3 and 4, and Form IIs, a
non-reproductive type with smaller chelae and lacking ischiopodite spines (Huner,
1994). We investigated the hormonal control of these transitions in two ways, by eyestalk
ablation, and by methyl farnesoate (MF) treatments. MF was quantitated by HPLC and
ecdysteroids by radioimmunoassay. Of 4 untreated Form I males all molted into Form
IIs, while 6 of 7 Form IIs molted into Form Is. After eyestalk ablation 8 of 8 Form
Is molted into Form IIs, while 5 of 5 Form IIs molted into Form IIs. MF treatment
of intact animals with MF (2 µg/animal/day) resulted in 6 of 7 Form Is becoming Form
IIs and 5 of 6 Form IIs becoming Form IIs (significant by chi square analysis P <
0.01). MF levels in premolt blood suggested that Form IIs are produced in the presence
of 1.3ng/ml MF, while Form Is result from MF levels of 0.5ng/ml. We conclude that
the control of morphogenesis of adult primary reproductives (Form Is) depends on low
levels of MF prior to the molt, while Form IIs are produced in the presence of elevated
levels of MF, since both eyestalk ablation and MF treatments result in the failure
of Form IIs becoming Form Is. (Supported by the Sea Grant College Program and CT.
DEP)
A comparison of in vivo and in vitro rates of juvenile hormone synthesis and degradation
in the lubber grasshopper
S. Li1, M.D. Knepp1, Y.C. Ouyang1, 2, J.D. Hatle1 and D.W. Borst1
1 Department of Biological Sciences, Illinois State University, Normal, IL 61790,
USA
2 Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
sli@ilstu.edu
To better understand how juvenile hormone (JH) levels are regulated, we developed
a novel method for estimating in vivo rates of both JH synthesis and degradation.
This method was used to determine these rates during the oviposition cycle of the
lubber grasshopper, Romalea microptera. These in vivo rates were compared to in vitro
measurements of JH synthesis by corpora allata (using a radiochemical assay) and JH
degradation by the hemolymph (using a partition assay). The rates of JH synthesis
in vivo paralleled those measured in vitro: JH synthesis was lowest at the beginning,
highest during the middle, and intermediate at the end of the oviposition cycle. However,
the estimated rates of JH synthesis in vivo were 3.4 to 33-fold higher than the rates
measured in vitro. The rates of JH degradation in vivo also paralleled those measured
in vitro: JH degradation was highest at the beginning, lowest near the middle, and
intermediate at the end of the cycle. However, the estimated rates of JH degradation
in vivo were 10−3 to 10−5 times lower than the rates measured in vitro. These results
confirm that changes of JH levels reflect variations in both JH synthesis and degradation.
This novel method provides a new approach to study the complex mechanisms involved
in regulating JH levels in insects. (Supported by NSF grant DBI - 9978810 to DWB).
Regulation of juvenile hormone synthesis in mosquito: physiological, biochemical and
molecular studies
Y. Li1, S. Hernandez-Martinez2, J.F Koener3, G.C. Unnithan3, R. Feyereisen3 and F.G.
Noriega1
1Department of Biological Sciences, Florida International University, Miami, FL 33199,
USA
2CISEI, INSP, Cuernavaca, Morelos, 62508, Mexico
3Department of Entomology, University of Arizona, Tucson, AZ 85721, USA
noriegaf@fiu.edu
We aim to understand the regulation of juvenile hormone (JH) synthesis in mosquitoes,
and how nutritional signals affect the activity of the neuroendocrine system. JH III
synthesis is low immediately after adult emergence, increases in sugar-fed females
and decreases after bloodfeeding. Aedes-allatotropin makes CA in newly emerged females
capable for JH biosynthesis. Anopheles gambiae PISCF-amide-allatostatin (allatostatin-C-type)
is very effective inhibiting JH synthesis. By raising larvae under different nutritional
diets two different adult phenotypes (large and small females) were generated. Teneral
reserves (protein, lipids and glycogen) were significantly lower in small females.
JH synthesis was significantly reduced in females emerged with low teneral reserves
and stimulated by sugar feeding; only when nutrients are appropriate the CA becomes
capable of synthesizing enough JH to activate reproductive maturation. EST were sequenced
from libraries made from corpora allata-corpora cardiaca complexes from Aedes aegypti
and Anopheles albimanus. A comparison with ESTs from the CA of Diploptera punctata
confirmed the identity of enzymes involved in JH synthesis and of other important
regulatory molecules.
Juvenile hormone activity of novel homo- and polyenehomobenzenes in fruit flies and
moths
M.W. Lorenz1, V. Rosales2, J.L. Zambrano3
1Department of Animal Ecology 1, University of Bayreuth, 95440 Bayreuth, Germany
2Universidad Central de Venezuela, Facultad de Farmacia, Caracas, Venezuela
3Universidad Simón Bolivar, Departamento de Química, Valle de Sartenejas, Baruta,
Caracas, Venezuela
matthias.lorenz@uni-bayreuth.de
Eight mono- and polyenehomobenzene compounds, synthesized via a highly regioselective
palladium-catalyzed ?-alkylation of allylic bromides and chlorides, with substituted
and unsubstituted benzylic Grignard reagents, were tested for their juvenile hormone
action on larvae and pupae of a dipteran (Drosophila virilis) and last instar larvae
of a lepidopteran species (Spodoptera frugiperda). Three dienes with a para-substituted
phenyl ring displayed a strong insecticidal activity against D. virilis, whereas mono-,
tri- and tetraenes as well as a nonsubstituted diene were not active. Hence, a para-substituted
phenyl ring and the length of the aryl chain seem to be important for insecticidal
action in D. virilis. In contrast, a monoene, a para-substituted diene and a para-substituted
triene were active against S. frugiperda. The same compounds were also active against
another lepidopteran, the map-butterfly Araschnia levana. Methoprene, which served
as a positive control, was active in both dipterans and lepidopterans. We conclude
that some of the tested compounds are specific against certain insect orders and may
thus serve as a starting point for the design of more specific juvenile hormone analogs
for insect pest control.
Molecular characterization and developmental expression of RXR in Blattella germanica
O. Maestro, D. Martin, J. Cruz and X. Belles
Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular
de Barcelona (CSIC), Jordi Girona 18, 08034 Barcelona, Spain
xbragr@ibmb.csic.es
We have cloned two isoforms of RXR in Blattella germanica, a short one (BgRXR-S) and
a longer one (BgRXR-L), which are identical except for a deletion/insertion of 23
amino acids in the loop between the helix 1 and 3 of the ligand binding domain. Transcript
levels of both isoforms were studied in the fat body of fifth and sixth larval stages,
in the ovary and fat body of females during the reproductive cycle and in embryos.
BgRXR-S predominates in fat body tissues, whereas BgRXR-L predominates in the ovary.
In larval fat body, the expression is quite constant along the stage and only shows
a modest peak during molting. In the adult female fat body, mRNA levels are quite
constant during vitellogenesis and slightly increase just before oviposition. Conversely,
ovarian mRNA levels slightly decrease towards the middle of vitellogenesis. In the
embryo, both isoforms show a differential expression, BgRXR-L transcripts being predominant
in the first three days and decrease thereafter, whereas those of BgRXR-S are low
during these first days, increase from days 3 to 5 and then remain practically constant.
Treatment of B. germanica embryo UM-BGE-1 cells with JH did not modify transcript
levels of any of the two isoforms.
Cloning of E75 nuclear receptors in Blattella germanica and functional characterization
in the fat body of during vitellogenesis
D. Mañé, D. Martin, J. Cruz and X. Belles
Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular
de Barcelona (CSIC), Jordi Girona 18, 08034 Barcelona, Spain
xbragr@ibmb.csic.es
We have cloned the ecdysone-inducible orphan nuclear receptor E75 in Blattella germanica.
Three isoforms, BgE75A, BgE75B and BgE75C, orthologues of the corresponding isoforms
of Drosophila melanogaster, were molecularly characterized and their expression was
studied in the fat body of adult females during the vitellogenic cycle. mRNA levels
of the three isoforms are low in the first days of adult age, increase from days 3
to 5–6 and decrease thereafter. The mRNA increase corresponds to the increase of circulating
ecdysteroids that occurs in parallel, and the general expression pattern of E75 looks
similar to that of vitellogenin production. However, the patterns of the three isoforms
are not completely parallel, the peak of BgE75B being one day delayed with respect
to BgE75A and BgE75C. These patterns and the occurrence of a putative E75 response
element in the promoter region of the vitellogenin gene of B. germanica suggest that
E75 might be involved in the regulation of the vitellogenic cycle. Results in line
with this hypothesis will be discussed.
Farnesyl dyphosphate synthase of insects targeted to mitochondria
D. Martin, M.D. Piulachs and X. Belles
Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular
de Barcelona (CSIC), Jordi Girona 18, 08034 Barcelona, Spain
xbragr@ibmb.csic.es
The NH2-terminal extension of pharnesyl-diphosphate synthase (FPS) sequence of Drosophila
melanogaster and Agrotis ipsilon have the structural features characterising mitochondrial
transit peptides. These extensions are enriched in basic, hydroxylated and hydrophobic
residues, contain a number of tetrapeptides fitting the consensus sequence RXXS, which
is currently reported as a mitochondrial targeting cleavage motif, and may form a
positively charged amphiphilic alphahelix. Functional studies using mutant yeast complementation
assays have shown that these extensions are able to convey a passenger protein into
yeast mitochondria. Moreover, Drosophila S2 cells transfected with EGFP showed a green
diffuse pattern covering nucleus and cytoplasm, whereas those transfected with the
NH2-terminal extension of D. melanogaster FPS fused to EGFP exhibited a typical mitochondrial
network pattern. The implications of FPS targeting into mitochondria in the context
of the regulation of JH biosynthesis is discussed.
Juvenile hormone diol kinase
R.A. Maxwell, W.H. Welch, F.M. Horodyski, R.J. Anderson, K.M. Schegg and D.A. Schooley
Department of Biochemistry, University of Nevada, Reno, Nevada 89557, USA
Robert.Maxwell@pnl.gov
The goal of this work was to isolate and characterize the enzyme responsible for producing
juvenile hormone diol phosphate, the principal end product of JHI metabolism in Manduca
sexta, and to investigate its role in juvenile hormone metabolism. This goal was accomplished
first by developing a simultaneous preparation of both enantiomers of juvenile hormones
labeled at C-10 with tritium at high specific activity. With substrate in hand, juvenile
hormone diol kinase (JHDK) was purified and characterized from Manduca sexta. Characterization
of JHDK indicated a nanomolar Km for JH I diol and a low micromolar value for MgATP.
JH II and III diols also serve as phosphate acceptors with low micromolar Km, whereas
other diol derivatives of terpenoid esters structurally similar to JH metabolites
are not phosphorylated. The gene sequence of JHDK codes for an enzyme that has 59%
sequence identity to Drosophila melanogaster sarcoplasmic calcium-binding protein-2
(dSCP2). Molecular modeling of JHDK and dSCP2 revealed structural similarity to G-proteins
and calcium binding proteins. We conclude that dSCP2 is a homolog of JHDK, and these
proteins constitute a novel kinase family that binds nucleotides using the scaffold
of a SCP.
Differential responses to allatostatin in migrant and non migrant populations of the
true armyworm, Pseudaletia unipuncta (Lepidoptera: Noctuidae)
J.N. McNeil1 and M. Cusson2
1 Biology, University of Western Ontario, London, ON. N6A 5B7, Canada
2Laurentian Forestry Centre, Sainte-Foy, QC, G1V 4C7, Canada
jmcneil2@uwo.ca
A long term interdisciplinary project has been examining the reproductive biology
of migrant (Quebec) and non-migrant (Azores) populations of the true armyworm, Pseudaletia
unipuncta. In this species, female sexual maturation is regulated by JH, whose titers
vary significantly in response to abiotic cues (temperature and photoperiod) associated
with habitat quality. Azorean individuals become sexually mature at a significantly
younger age than those from Quebec, and despite having a lower body mass they have
a significantly higher lifetime fecundity. However, an examination of JH biosynthesis,
as well as JH and JH esterase titers in the hemolymph, clearly shows that the difference
between the two populations results from more than just a simple temporal shift for
earlier, post-emergence, JH production in the non-migrant population. In addition,
new data examining the responses of the corpora allata (CA) from different-aged Quebec
and Azorean females to a fixed dose of allatotropin, as well as those from early and
late maturing lines selected from the Quebec population, also support the idea that
there are complex differences in the reproductive physiology of migrant and non-migrant
populations.
Increased juvenile hormone levels after long-duration flight in the grasshopper, Melanoplus
sanguinipes
K. J. Min1,2, N. Jones2, D. W. Borst3 and M. A. Rankin2
1Department of Ecology and Evolutionary Biology, Brown University, Providence, RI,
USA
2Integrative Biology, University of Texas, Austin, TX, 78712 USA
3Department of Biological Sciences, Illinois State University, Normal, IL 61790 USA
Kyung-Jin_Min@brown.edu
While migration imposes a cost to reproduction in many insects, in the migratory grasshopper
Melanoplus sanguinipes long duration flight accelerates the onset of first reproduction
and enhances reproductive success over the entire lifetime of the insect. Since juvenile
hormone (JH) is involved in the control of reproduction in most insects, we examined
JH titers after long flights using a chiral selective radioimmunoassay. JH levels
increased in animals flown to exhaustion but not in animals that were flown for short
durations (one-hour) or not flown. No differences were observed in the diel pattern
of JH titers associated with flight performance. Treatment of grasshoppers with JH
III mimicked the effect of long duration flight in the induction of early reproduction.
To test the possibility that the post-flight increase in JH titer is caused by adipokinetic
hormone (AKH), we injected unflown animals with physiological doses of LocmiAKH I
to simulate the release of AKH during long flight. This treatment had no effect on
JH titers. Grasshopper feeding and digestion was measured in response to flight experience
and long-duration fliers displayed increased feeding and lipid digestion. The relationship
between feeding and JH levels is being explored. (Supported by NSF grant 0235892 to
MAR).
Regulation of juvenile hormone titers in Coptotermes formosanus termite by different
soldier percentages
Y.I. Park and A. K. Raina
Formosan Subterranean Termite Research Unit, USDA-ARS-SRRC, 1100 Robert E. Lee Blvd.
New Orleans, LA, 70124, USA
ypark@srrc.ars.usda.gov
In the Formosan subterranean termite Coptotermes formosanus, the soldier caste represents
less than 10%, on average, of field collections. In the laboratory, the number of
soldiers increased to about 25% or higher, and workers and soldiers appeared to increase
JH III titers. In lab experiments with groups containing 25% or higher soldiers, few
workers were transformed into pre-soldiers and subsequently to soldiers. The JH titer
in these soldiers remained constant. Soldiers and workers from groups containing less
than 25%-soldiers showed higher JH titers. Newly formed soldiers had higher JH titers
than older soldiers. Groups containing 25% or higher soldiers inhibited further soldier
differentiation by possibly suppressing the activity of the corpora allata, eventually
showing lower JH titers in workers.
Pharmacological analysis of ovarial patency in Heliothis virescens
M.A. Pszczolkowski1, S. Ramaswamy1 and A. Srinivasan2
1Department of Entomology, Kansas State University, Manhattan, KS, 66506, USA
2Tougaloo College, Tougaloo, MI, 39714, USA
pszcz@ksu.edu
In insects, six homologues of juvenile hormone have been identified, including juvenile
hormones (JH) 0, 1, 2, 3, bisepoxide-3, and isomethyl-1. The lepidopterans, particularly
moths, are unique in being the only group of insects in which, except for JH-B-3,
all of the other homologues have been demonstrated. Using reproduction in Heliothis
virescens as a model system, we have initiated studies to explain the mechanisms for
why moths exhibit multiple JH homologues. As part of these studies we are attempting
to determine the JH signal transduction process in the patency of the follicular epithelium.
We used pharmacological agents to analyze signaling pathways that JHs use to cause
in vitro ovarial patency in H. virescens. Our results suggest that, similar to Rhodnius
prolixus, NaK-ATP-ase is involved in response to JHs in H. virescens as well. However,
in contrast to R. prolixus, at least two kinds of protein kinases appear to mediate
the signal transduction process in H. virescens. Potential roles of G protein and
internal calcium stores are being examined. We will present data on these experiments
and discuss the situation in H. virescens compared with that in R. prolixus and other
insects.
Inhibition of vitellogenesis by the chico1
insulin-signaling pathway mutation of Drosophila melanogaster does not involve systemic
juvenile hormones and ecdysteroids
D.S. Richard1 and L.G. Harshman2
1Department of Biology, Susquehanna University, Selinsgrove, PA, 17870, USA
2School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68588,
USA
richard@susqu.edu
Chico1
is a null mutation in the insulin-receptor substrate protein (CHICO) gene of Drosophila
melanogaster. Females are sterile and their oocytes do not mature beyond the last
previtellogenic stage. We show that the sterility of chico1 females is not attributable
to systemic factors, but instead to an autonomous ovarian phenotype. Chico1
females exhibit approximately wild type rates of juvenile hormone (JH) biosynthesis,
and relatively high ovarian and hemolymph ecdysteroid levels. Wild type ovaries transplanted
into chico1
females underwent vitellogenesis, indicating that systemic factors present in chico1
mutant females are sufficient to support normal vitellogenesis. Chico1
ovaries transplanted into wild type females did not undergo vitellogenesis indicating
that CHICO is necessary in the ovary for vitellogenic maturation. We suggest that
a failure of receptor-mediated endocytosis of yolk protein could contribute to the
molecular basis of chico1
female sterility. The global organizing hormones in insects, JHs and ecdysteroids,
have long been thought to exert overall control on vitellogenesis. However, this work
implies that irrespective of systemic regulatory roles of JHs and ecdysteroids, insulin
signaling is necessary for vitellogenic oocyte maturation. Supported by NIH GM/OD54905
(DSR) and NIA AG08761, NIH 1R24GM65513-01(LGH)
Parasitism by Glyptapanteles liparidis (Hym., Braconidae) alters the juvenile hormone
metabolism of its host larva, Lymantria dispar
C. Schafellner, R.C. Marktl and A. Schopf
Institute of Forest Entomology, BOKU -University of Natural Resources and Applied
Life Sciences, Vienna, Austria
Axel.Schopf@boku.ac.at
As shown earlier, parasitization by the larval parasitoid G. liparidis induces a dramatic
increase in the hemolymph juvenile hormone (JH) titer of its host larva, especially
in the JH III homologue, while the activity of the JH degrading enzyme (JHE) is reduced
compared to unparasitized controls. Here we investigated the role of the parasitoids
and associated factors in JH synthesis and degradation. GC-MS analysis confirmed that
the rising hemolymph JH titers coincided with the parasitoids' molt into 2nd instars.
Peak values were observed prior to parasitoid emergence from the host larva and titers
dropped to negligible levels shortly afterwards. Whole body extracts from 2nd instar
parasitoids yielded JH III and trace amounts of JH II. When the host's corpora allata
were separated by neck ligation, levels of JH III were elevated in the hemolymph of
the posterior section, which contained the parasitoids, but no JH II was found. When
parasitoids were kept in in vitro culture, they produced and released only JH III.
The parasitoids, on the other hand, had little influence on JH degradation. JHE suppression
was induced solely by the polydnavirus/venom complex which is injected into the host
larva at oviposition. Host JHE gene expression was not affected by parasitization.
Cloning and functional analysis of juvenile hormone acid methyltransferases from the
silkworm, Bombyx mori, and the fruitfly, Drosophila melanogaster
T. Shinoda1, K. Itoyama2 and T. Niimi3
1National Institute of Agrobiological Sciences, Oowashi, Tsukuba, Ibaraki, 305-8634,
Japan
2Akita Prefectural Agricultural Experiment Station, Yuwa, Kawabe, Akita, 010-1231,
Japan
3Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 461-8601,
Japan
shinoda@affrc.go.jp
Juvenile hormone (JH) acid O-methyltransferase (JHAMT) is the enzyme that converts
JH acids to JH methylesters at the final step of JH biosynthetic pathway. We have
recently isolated, by differential display, a cDNA encoding JHAMT from the corpora
allata (CA) of the silkworm Bombyx mori (BmJHAMT). The expression profile of BmJHAMT
gene in the CA during the penultimate and the last larval stages suggests that this
gene is a key regulator of JH biosynthesis at the onset of the metamorphosis in the
silkworm. We have further identified a homolog in the genome sequence of Drosophila
melanogaster (CG17330; DmJHAMT). Although the identity of the amino acid sequence
between DmJHAMT and BmJHAMT is quite low (∼35%), the recombinant DmJHAMT protein produced
in E. coli indeed converted JH acids to their cognate methylesters. In addition, immunohistochemistry
with an antiserum raised against the recombinant protein revealed the localization
of DmJHAMT protein in the CA cells of the larval ring gland. Thus DmJHAMT gene encodes
a genuine JHAMT working in the CA cells. Functional analysis of DmJHAMT gene in vivo
is now in progress using the ectopic expression and the inducible RNAi.
Juvenile hormone-binding protein at follicle cells in Bombyx mori
T. Shiotsuki1, A. Seino1 and Y. Kim2
1National Institute of Agrobiological Sciences, Tsukuba 305-8634, Japan
2Andong National University, Andong, 760-749, Korea
shiotsuk@affrc.go.jp
A novel JH binding protein with possibile relationship to hormone signal transduction
was identified from silkworm follicle cells. The follicle cells at a stage of pre-choriogenesis
were incubated with JHs and related compounds, and showed concentration-dependent
patency in contrast with no effect with 20-hydroxyecdysone. To characterize this biological
activity, the membrane fraction of the silkworm follicle cell membrane was prepared
and examined for binding activity with JH and analogous compounds. Two JH binding
assays were employed, dextran-coated charcoal (DCC) assay with tritiated JH III, and
a competitive assay with a biotinylated affinity ligand (phenoxyphenoxypropyl biotine)
partial structure of JH analogs, fenoxycarb and pyriproxyfen. The highest activity
of the binding activity was found at late pupal stage (day 6–8). Ligand binding was
inhibited competitively with excess of non-labeled JH. The dissociation constants,
KD values were 2.56, 1.77, and 4.32 for JHI, II, and III, respectively with the DCC
assay. The binding activity was lost after heating at 100° C for 10 min, or by protease
treatment of the follicle cell preparation.
Putative allatostatin peptides and their receptors in Caenorhabditis elegans: Analysis
of function through RNA inhibition
S. Slater1, I. Chin-Sang1, S.S. Tobe2 and W.G. Bendena1
1Department of Biology, Queen's University, Kingston, ON, Canada
2Department of Zoology, University of Toronto, Toronto, ON, Canada
slaters@biology.queensu.ca
Allatostatins are a group of peptides initially identified in Diploptera punctata,
which function to inhibit juvenile hormone synthesis and gut motility. Since their
discovery, allatostatin homologues have been identified in various other arthropods
as well as other invertebrates including Hydrozoa, Nematoda, Mollusca, and Platyhelminthes.
Allatostatins function in these latter invertebrates varies but can involve inhibition
of muscle contraction, inhibition of interneural and endocrine functions, as well
as direct action on biosynthetic pathways. There are, however, many organisms for
which allatostatin function has not been assigned. This includes the nematode Caenorhabditis
elegans. The C. elegans genome project has identified a gene termed nlp-6 whose precursor
polypeptide contains peptide sequences that are similar to allatostatin peptides.
Our current research is aimed at determining the function of nlp-6 and its potential
receptor ZK455.3 using RNA inhibition and GFP analysis. Preliminary results from RNAi
screens suggest increased egg laying rates in RNA inhibited nlp-6 and ZK455.3 worms.
Localization of nlp-6 has previously been shown in somatic gonad tissue. The mechanism
of action of these peptides is unclear but it is interesting that these peptides have
a role in reproduction as has been found in insect model systems.
Enzymology of moth farnesyl diphosphate synthase: Implications for the biosynthesis
of homologous juvenile hormones
A. Sperry, S. Sen, D.C. Brown and J. Hitchcock
Department of Chemistry, Indiana U.-Purdue U. Indianapolis, 402 North Blackford Street,
Indianapolis, IN, 46202, USA
sen@chem.iupui.edu
Juvenile hormone biosynthesis is derived from the mevalonate pathway and requires
the intermediacy of farnesyl diphosphate (FPP). FPP synthase (FPPS), the presumed
enzyme responsible for sesquiterpenoid construction in insects, is a metal-dependent
homodimeric protein, that catalyzes the sequential 1–4′ condensation of two molecules
of isopentenyl diphosphate (IPP) with the allylic primer, dimethylallyl diphosphate
(DMAPP). Additional complexity for FPPS catalysis is expected to exist in moths, which
are known to produce homologous JH structures. We have studied FPPS activity located
within the corpus allatum of larval and adult Manduca sexta. The two developmental
stages show several differences, including enzyme localization, detergent activation,
metal preference, substrate specificity, and kinetic behaviour. These properties are
distinct from those observed using purified FPPS obtained from whole body M. sexta
and porcine FPPS, suggesting that the isoprenyltransferase within the corpus allatum
has unique enzymological properties.
Regulation of Reproductive Development in the boll weevil, Anthonomus grandis
T. E. Taub-Montemayor1, K. J. Min2 and M. A. Rankin1
1Integrative Biology, University of Texas, Austin, TX 78712
2Department of Ecology and Evolutionary Biology, Brown University, Providence, RI,
02912
ttaub@mail.utexas.edu
Taub-Montemayor, et al. (1997) showed a positive correlation between juvenile hormone
(JH) synthesis and ovarian development in the boll weevil, Anthonomus grandis. The
production of vitellogenin (Vg) is typically restricted to reproductively competent
females and no Vg was detected in untreated male boll weevils (Taub-Montemayor & Rankin,
1997). Methoprene stimulates Vg production in the isolated abdomens of both female
and male boll weevils (Taub-Montemayor and Rankin, 1997), suggesting that males are
competent to produce Vg but are normally not stimulated to do so. Preliminary work
indicated that male boll weevil CA produce little or no JH in vitro, suggesting that
males may not produce Vg because of very low JH levels. Reports of JH effects in male
A. grandis induced us to more carefully monitor JH synthesis, degradation, and circulating
titres in male A. grandis during early adult development. We also examined JH titres
in females and re-examined the ability of males to produce Vg with and without hormonal
stimulation. Results indicate that male JH titers and JH production are similar to
females. The sexually dimorphic effect of JH on Vg production is thus not due to differences
in JH production or titers between the sexes.
Regulation of methyl farnesoate production in crayfish: A possible role for allatostatins
S.S. Tobe, J.R. Zhang, and R. Kwok
Department of Zoology, University of Toronto, Toronto, ON, Canada M5S 3G5
rodney.kwok@utoronto.ca
Decapod crustaceans do not appear to produce juvenile hormone (JH III), but rather
its immediate precursor, methyl farnesoate (MF). Both MF and its immediate precursor,
farnesoic acid (FA) are produced by the mandibular organs (MOs) in crustaceans. The
MOs are homologous to the insect corpora allata (CA), the site of juvenile hormone
biosynthesis. However, the FGLa ASTs, of which there are about 60 distinct peptides
reported from crustaceans, have previously been found to have no effect on MO activity
in crustaceans. In light of our recent report on MF production in embryonic CA of
the cockroach, Diploptera punctata and its modulation by ASTs, we have reexamined
the ability of selected FGLa ASTs to modulate MF production in the crayfish, Procambarus
clarkii. Using MO from adult males of, we have found wide variability between animals
in the in vitro rates of MF and FA synthesis. Treatment with Dippu-ASTs has a statistically
significant stimulatory effect on MF synthesis, but only in MO that are initially
producing MF at lower rates. No effect on FA production was observed, suggesting that
the FGLa ASTs exert their effect on the o-methyl transferase, the enzyme responsible
for the conversion of FA to MF.
Insulin signal regulation of juvenile hormone synthesis in Drosophila melanogaster
M.-P. Tu1, C.-M. Yin2 and M. Tatar1
1Department of Ecology and Evolutionary Biology, Brown University, Providence, RI,
02912
2Department of Entomology, University of Massachusetts, Amherst, MA, 01003, USA
Meng-Ping_Tu@brown.edu
Juvenile hormone is a key endocrine regulator of metamorphosis, reproduction, and
aging in Drosophila melanogaster. Mutants of the insulin receptor (InR) and the insulin
receptor substrate homolog (chico) are slow to develop, small, infertile, and long-lived.
Here we describe the 10-day adult age course of JH synthesis from isolated corpora
allata of InR and of chico. JH synthesis increased in wildtype flies to a maximum
of 30 fmol/gland/h at day 10. In contrast, homozygous InR mutants produced only 7
fmol/gland/h at 10 days. InR mutation disproportionately reduced the synthesis of
JH III-bisepoxide, the major JH subtype of the fly. Both homozygous and heterozygous
chico genotypes reduced JH synthesis, but only to 47% and 67% of wildtype synthesis,
respectively, and without influencing the ratio of JH subtypes. Because JH synthetic
rate does not correlate with the size of CA among chico genotypes, insulin signaling
appears not to influence JH by impeding tissue development. Rather, allatotropic positive
axons are abundant in the adult brain but less immunoreactive in the InR mutant genotype,
suggesting that insulin signaling may affect JH synthesis through control of neuropeptides.
Influence of larval haemolymph, JH acids and long term incubation with allatotropin
analogue on juvenile hormone biosynthesis in Manduca sexta : In vitro studies
B.G. Unni
Biochemistry Department, Regional Research Laboratory (CSIR) Jorhat-785006. Assam,
India
bgunni@yahoo.com
0 day Vth instar CA were incubated with the medium for 3hrs containing IIIrd and IVth
instar larval haemolymph, no stimulatory effect of JH biosynthesis was observed. However
exogenous addition of pharate Vth haemolymph with medium-199 and pharate Vth haemolymph
alone increases the JH production by four times than the control. The addition of
JH acids with the pharate haemolymph increased the JH production to 14.97 pmol/h/prCA,
which is about 15 fold more than the control. The degree of activation of corpora
allata with allatotropin analogue was about 4 times for 3–6 hours interval and about
6 times in both 6–9 and 9–12 hrs under the same incubation condition. The preliminary
experiments may suggest the possible existence of a CA stimulatory factor in the pharate
Vth instar haemolymph of M. sexta. Dr.B.G.Unni is thankful to Director for permission
to present this research work at the conference and also to Professors G. Bhaskaran,
K.H. Dahm and Tim Hayes for their collaboration and constant guidance to carry out
this piece of research work at the Biology Department, Texas A&M University, USA and
United States Educational Foundation in India for Fulbright Fellowship travel Grant.
Regulation of methyl farnesoate synthesis in mandibular organs of the crustacean,
Cancer pagurus by mandibular organ – inhibiting hormones
G. Wainwright1, C.Tang1, W. Lu1, C.J. Ruddell1, P.C. Turner1, S.G. Webster2 and H.H.
Rees1
1School of Biological Sciences, University of Liverpool, Biosciences Building, Crown
St., Liverpool, L69 7ZB, U.K.
2School of Biological Sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, U.K.
reeshh@liverpool.ac.uk
In Crustacea, there is evidence that methyl farnesoate (MF) acts as a juvenile hormone.
MF is synthesized in the mandibular organs (Mos) under the negative control of the
neuropeptides, mandibular organ-inhibiting hormones (MO-His), which are produced and
secreted from the X organ-sinus gland compex of the eyestalk. The latter 78 amino
acid residue peptides, which were purified from C. pagurus, are members of the crustacean
hyperglycemic hormone (CHH) family and their expression is confined to the X-organ.
Phylogenetic analysis and gene organization show that MO-IH and MIH (moult-inhibiting
hormone) genes are closely related and represent evolutionary divergence of crustacean
hormones. We have shown that MO-IH inhibits farnesoic acid (FA) O-methyl transferase
(FAMTase), the enzyme that catalyzes the final step of MF biosynthesis. Levels of
putative FAMTase transcripts in Mos of female C. pagurus fluctuated during vitellogenesis
and embryonic development. Evidence strongly supports a role for cAMP in the signal
transduction mechanism of MO-IH that leads to inhibition of MF synthesis in Mos.
Type-B [W(X6)Wamide] allatostatins from the cricket, Gryllus bimaculatus: molecular
cloning, expression and tissue-specific localisation
J. Wang, M. Meyering-Vos, and K.H. Hoffmann
Department of Animal Ecology I, University of Bayreuth, 95440 Bayreuth, Germany
klaus.hoffmann@uni-bayreuth.de
Five W(X6)Wamides which inhibited JH biosynthesis in vitro of the corpora allata were
isolated from cricket brains in a previous work. In addition to their allatostatic
function, two of the peptides effectively inhibited ecdysteroid biosynthesis in the
ovaries from adult females. Here we report on the partial identification of the cricket
allatostatin type-B gene, and experiments were performed to demonstrate spatial and
temporal expression patterns of the gene in various tissues of adult crickets. By
PCR screening of a random primed adaptor ligated cDNA library and by RACE, a 535 bp
3′cDNA fragment was yielded which encodes a putative translation product of 85 amino
acids containing six peptides of the allatostatin B-type. By Southern blot analyses
it was proven that the gene is expressed as a single copy per haploid genome. Using
RT in situ PCR with 10µm tissue sections and RNA dot blot analyses, it was demonstrated
that the gene is expressed, for example, in brain, ovary and digestive tract of adult
crickets. In the germarium and in primary oocytes, gene expression was detected as
condensed signals that changed into separated granules and finally disappeared during
oogenesis, whereas in the follicular cells strong signals became apparent. Supported
by the DFG (Ho 631/15-4).
Rapid quantification of juvenile hormones (JH) and their metabolites in the haemolymph
of insects by liquid chromatography-mass spectrometry (LC-MS)
S.A. Westerlund and K.H. Hoffmann
Department of Animal Ecology I, University of Bayreuth, 95440 Bayreuth, Germany
klaus.hoffmann@uni-bayreuth.de
Various methods have been developed to determine JH titres in insects by immunological
or physicochemical techniques. Physicochemical methods such as gas chromatography-mass
spectrometry (GC-MS) have been considered to be most accurate, but extensive sample
preparation has been a major disadvantage when using GC-MS for routine analyses, followed
by the inability to monitor JH degradation products. Here we present a simple, fast
and sensitive method for routine determination of JHs, JH-diols, and JH-acids in insect
haemolymph by LCMS. Sample clean-up involves the precipitation of proteins by methanol-isooctane
(1:1, v/v), centrifugation, and partial evaporation of the solvents. The JH compounds
were separated on a ReproSil-Pur ODS-3 C18 column by gradient elution with water and
methanol in less than 22 min and analysed by electrospray MS (ESI-MS). The limit of
detection and quantification was 6 and 20 pg for JHs, and 8 and 25 pg for JH-diols,
respectively. To demonstrate the applicability of the method, haemolymph samples from
crickets, moths, aphids and ants were analysed. Our results reveal that in the haemolymph
of cricket adult females, besides JH III significant amounts of JH I are present.
Supported by the DFG (GK 678/1).
Crystal structure of juvenile hormone esterase from Manduca sexta, with the inhibitor
OTFP covalently bound in the JHE active site
M. Wogulis1, S.G. Kamita2, A.C. Hinton2, D.K. Wilson1 and B.D. Hammock2
1Department of Molecular and Cellular Biology
2Department of Entomology and Cancer Research, University of California, Davis, CA,
95616
mdwogulis@ucdavis.edu
Juvenile hormone esterase (JHE) is a selective and highly efficient enzyme that hydrolyzes
the methyl ester of juvenile hormone. JHE belongs to the a/?–hydrolase family of enzymes,
which is a large family of enzymes with diverse substrates and a conserved catalytic
mechanism. This mechanism involves a nucleophilic attack on the substrate, leading
to release of the methyl alcohol and formation of an acyl-enzyme intermediate via
a tetrahedral transition state intermediate. This acyl-enzyme intermediate is then
hydrolyzed, leading to release of the JH acid and regeneration of enzyme. We present
a 2.8 Å crystal structure of JHE from the lepidopteran Manduca sexta. Crystals were
produced with the covalent inhibitor 3-octylthio-1,1,1-trifluoro-2propanone (OTFP)
present, and the inhibitor is clearly present in this crystal structure. The inhibitor
mimics the tetrahedral acyl-enzyme intermediate, and positioning of OTFP indicates
how the substrate likely binds. This provides insight into the specificity of the
enzyme and the potency of various inhibitors. Mutations have been introduced into
this protein to help us further understand the catalytic pathway of this highly efficient
enzyme.
Allatostatin in the termite Reticulitermes flavipes: Content in brain and corpus allatum
and effect on juvenile hormone synthesis
K.J. Yagi1, R. Kwok1, K.K. Chan2, R.R. Setter3, T.G. Myles4, S.S. Tobe1 and B. Stay2
1Department of Zoology, University of Toronto, Toronto, Ontario., M5S 3G5, Canada
2Department of Biological Sciences, University of Iowa, Iowa City, IA, 52242, USA
3Facilities Services Group, University of Iowa, Iowa City, IA, 52242, USA
4Centre for Urban and Community Studies, University of Toronto, Ontario, M5S 2G8,
Canada
k.yagi@utoronto.ca
In the subterranean termite Reticulitermes flavipes, allatostatins (ASTs) with the
C-terminus PheGly-Leu amide, were localized by immunocytochemistry with antibody against
cockroach AST. AST-immunoreactivity occurred in the corpus cardiacum and corpus allatum
and in the lateral and medial neurosecretory cells of the brain that innervate these
organs as well as in many other nerve cells of the brain. This was observed in workers,
nymphs, soldiers and secondary reproductives. A radioimmunoassay demonstrated about
40 fmole equivalents of Dippu AST-11 in brains of soldiers and secondary reproductives.
The product of the corpora allata in this species was determined to be juvenile hormone
III. Its synthesis by corpora allata of secondary reproductives, determined by in
vitro radiochemical assay, was inhibited in a dose dependant manner by two cockroach
allatostatins, Dippu AST-7 and Dippu AST-11. Thus, as in cockroaches and crickets,
AST-containing nerves innervate the corpora allata of this termite species and their
production of juvenile hormone is inhibited by these neuropeptides.
The hemolymph JH titer exhibits a large-amplitude, morph-dependent, diurnal cycle
in Gryllus species in the laboratory and field
A. Zera and Z. Zhao
School of Biological Sciences, University of Nebraska, Lincoln, NE, 68588, USA
azera1@unlnotes.unl.edu
We observed a striking, morph-dependent daily cycle in the hemolymph JH titer in the
cricket Gryllus firmus in both the laboratory and the field. In the flight-capable
(long-winged, LW) morph, the JH titer rose about 10–100-fold a few hours before lights-off
(lab) or sunset (field) and dropped precipitously just after lights-off or sunset.
By contrast, the JH titer was temporally invariant in the flightless (short-winged,
SW) morph. The JH titer cycle was lost in LW individuals that became flightless due
to flight-muscle histolysis. No morph-dependent cycle was observed for the hemolymph
ecdysteroid titer. A similar morph-dependent diel cycle for the JH titer was observed
in four other cricket species. This represents the most dramatic case of diel variation
in the JH titer in an insect, the only case where the titer cycle is morph-dependent,
and the only characterization of the JH titer under field conditions. The morph-dependent
JH titer cycle has important implications for the endocrine basis of morph specialization,
especially the regulation of morph-specific nocturnal flight, and JH endocrinology
in general.
A morph-dependent daily cycle of JH biosynthesis underlies a morph-dependent daily
cycle of the hemolymph JH titer in a wing-polymorphic cricket
Z. Zhao and A. Zera
School of Biological Sciences, University of Nebraska, Lincoln, NE, 68588, USA
zhaozw_00@yahoo.com
A dramatic morph-specific daily cycle in rate of JH biosynthesis that covaried tightly
with a daily cycle in the hemolymph JH titer was observed in the wing-polymorphic
cricket, Gryllus firmus. In the flight-capable (LW, long-winged) morph, rate of JH
biosynthesis rose 1–3 fold and the JH titer rose 10–100 fold near the end of the photophase,
while the rate of JH biosynthesis fell an equivalent amount, as did the JH titer,
during the beginning of the scotophase. By contrast, both rate of JH biosynthesis
and hemolymph JH titer were relatively invariant during this period in the flightless,
SW morph. An equivalent biosynthetic cycle was observed when assays were performed
with corpora allata alone, with corpora allata and attached corpora cardiaca, and
in males and females. The LW morph also exhibited significantly elevated juvenile
hormone esterase activity, mainly during the period when the JH titer was dropping.
To our knowledge, this is the first example, of a diurnal cycle in JH biosynthesis,
and one that is strongly associated with a particular morph in a wing-, caste-, or
phase-polymorphic insect.
CG14709 putatively mediates the juvenile hormone effect on preventing bristle outgrowth
in the developing adult Drosophila abdomen
X-F. Zhou and L.M. Riddiford
Department of Biology, University of Washington, Box351800, Seattle, WA 98195-1800
USA
xfzhou@u.washington.edu
Juvenile hormones (JH) are not able to prevent the larval-pupal transition in Drosophila
and other higher flies, unlike that seen in the Coleoptera and Lepidoptera. Yet exogenous
JH prevents the outgrowth of adult bristles and causes the formation of second pupal
cuticle on the abdomen. We have shown that the formation of the second pupal cuticle
is mediated by the transcription factor broad. However, loss-of-function analyses
suggest that broad is involved in bristle specification but not outgrowth. We recently
found three P element insertion mutants that are able to form normal bristles even
when given a high dose of JH. Interestingly, broad is still up-regulated by exogenous
JH in these mutants, indicating that JH effects on the developing adult abdomen are
likely mediated by two distinct pathways. All these P element insertions have been
mapped cytologically to the 86E14 region which hosts two genes: CG14709 and CG31305.
Our preliminary data show that the expression pattern of CG31305 is not altered by
these P element insertions. We are now determining if the expression of CG14709 is
changed in these insertion mutants. The finding of these JH resistant mutants provides
new opportunities of revealing the mechanism of JH action.
Isolation and characterization of a novel chymotrypsin-like protease gene that is
activated by JH III in mosquito Aedes aegypti
J. Zhu, L. Chen, A.S. Raikhel
Department of Entomology and Institute for Integrative Genome Biology
University of California, Riverside, CA 92521, USA
zhujin@ucr.edu
In order to isolate mosquito genes that are specifically up-regulated by JH III in
newly-emerged female mosquitoes, we exploit the suppression subtractive hybridization
(SSH) technique. Here we report the cloning and characterization of JA15, a chymotrypsin-like
protease gene. JA15 mRNA exhibits over a 10-fold increase in response to 10−6 M JH
III. This hormonal response is blocked by the protein synthesis inhibitor, cycloheximide
(CHX), suggesting that novel protein synthesis was obligatory for this hormonal effect.
Expression of JA15 in vivo is limited almost exclusively to the midgut. In adult females,
the JA15 transcript is scarce at eclosion, and starts to increase approximately 12
h post eclosion (PE). It reaches a plateau by 36 h PE, and remains at a nearly constant
level until a blood meal is taken. JA15mRNA decreases shortly after feeding, and remains
at relatively low levels until 48 h post blood meal (PBM), then begins to increase
again. By 72 h PBM, expression of JA15 is comparable to that before blood meal. The
expression profile of JA15 is closely correlated with the change in JH titers during
vitellogenic cycles, suggesting that the transcription of JA15 in previtellogenic
midgut is under the control of JH III.