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      Perfil fisiometabólico de perros Pastor Belga Malinois y Pastor Holandés de alto rendimiento en respuesta a una sesión de entrenamiento para Ring Francés Translated title: Physiometabolic profile of Belgian Malinois Shepherd and Dutch Shepherd dogs in response to a training session for French Ring

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          Abstract

          En el presente estudio se valoró la respuesta fisiometabólica a una sesión de entrenamiento de Ring Francés en dos razas de perros de alto rendimiento. Se utilizaron 20 perros, machos y hembras de las razas Pastor Belga Malinois y Pastor Holandés entre 10 y 24 meses de edad con pesos entre 30 y 35 kg y que fueron sometidos durante 20 minutos a una rutina de Ring Francés. La toma de muestras se realizó en tres períodos: antes del entrenamiento, inmediatamente después y 1 h postentrenamiento. Las muestras sanguíneas se obtuvieron de la vena cefálica. Se determinaron los niveles de lactato, pH, calcio, potasio y gases sanguíneos (pCO2 y pO2) mediante un analizador de variables críticas sanguíneas. Se obtuvieron la frecuencia cardiaca y temperatura corporal. En ambas razas la temperatura, pO2, pCO2, lactato y pH incrementaron (P < 0,05) después del período de entrenamiento, y disminuyeron 1 h después (P < 0,05). La raza Pastor Belga Malinois mostró un incremento (P < 0,05) en la Frecuencia Cardiaca en el postentrenamiento, y posteriormente una recuperación (P < 0,05) hacia los valores de inicio. La raza P. Holandés presentó una disminución (P < 0,05) en los niveles de calcio posterior al entrenamiento, manteniéndose sin cambios en la recuperación. En conclusión, estos datos permitirán contar con antecedentes bioquímicos y fisiológicos para poder evaluar y estimar el potencial de rendimiento competitivo y lograr una mejor selección en las dos razas de perros más empleadas en el Ring Francés.

          Translated abstract

          This study evaluated the physiometabolic response to a training session for French Ring in two high performance breeds of dogs to. Twenty male and female Belgian Malinois Shepherd and Dutch Shepherd dogs were used. Animals aged 10 to 24 months and weighing between 30 and 35 kg (66-77 lbs) performed a French Ring routine for 20 minutes. Blood samples were taken at three moments: prior to training, immediately after exercise, and then 1 h later. All samples were drawn from the cephalic vein. Lactate, pH, calcium, potassium and blood gas (pCO2 and pO2) levels were determined using a critical blood variables analyzer. Heart rate and body temperature were also recorded. In both breeds, temperature, pO2, pCO2, lactate and pH levels increased (P < 0.05) immediately after the training period, but decreased 1 h after exercise (P < 0.05). The Belgian Malinois Shepherd dogs showed an increase (P < 0.05) in cardiac frequency in the post-training period, with a later recovery (P < 0.05) that tended towards initial values. The Dutch Shepherd breed displayed a decrease (P < 0.05) in calcium levels after training that remained without change during recovery. In conclusion, these results provide biochemical and physiological data that will make possible to evaluate and estimate the potential for competitive performance of these breeds, improving the selection of these two dog breeds.

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          Tratado de fisiologia médica

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            Hematologic, serum biochemical, and cortisol changes associated with anticipation of exercise and short duration high-intensity exercise in sled dogs.

            Many studies have demonstrated changes in clinicopathologic variables in response to exercise in athletic dogs. These changes have been attributed to the duration and intensity of exercise. However, inconsistencies in the timing of collection of resting blood samples can be a confounding variable when interpreting results. The objectives of this study were to determine whether hematologic and biochemical data in sled dogs are influenced by (1) anticipation of an exercise event or (2) short-duration high-intensity exercise. Blood samples were collected into EDTA tubes and tubes without anticoagulant from sled dogs at rest, immediately before a 3-mile run (preexercise), and immediately after the run (postexercise). A CBC, biochemical profile (including electrolytes, glucose, proteins, creatinine, urea, enzymes, cholesterol, and total bilirubin), and serum cortisol concentration were measured using standard methods on automated analyzers. Significant increases in sodium, chloride, albumin, calcium, and cortisol concentration were observed in preexercise samples when compared with resting samples. Hyperglycemia and a further rise in serum cortisol concentration occurred immediately postexercise. The results of this study indicate that anticipation of exercise affects several biochemical analytes, emphasizing the importance of appropriate timing of baseline blood samples. The pronounced hyperglycemia immediately after short-duration exercise may be a breed- or exercise-dependent phenomenon.
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              End points of lactate and glucose metabolism after exhausting exercise.

              To determine the extent of metabolite oxidation, rats were injected with [U-14C]lactate, -glucose, or -bicarbonate (n = 5, each) during rest or after continuous (CE) and intermittent (IE) exercises to exhaustion. Tissue analyses of resting rats, or rats killed following CE and IE and pulse injection with [14C]lactate or -glucose (n = 72, each), were used to determine the metabolic pathways of these two substrates. Oxygen consumption (VO2) declined rapidly for the first 15 min after exercise; thereafter, VO2 declined slowly and remained elevated above resting levels for 120 min. The slow phase of decline in VO2 during recovery did not coincide with lactate removal, which occurred within 15 min. Two-dimensional radiochromatograms produced from blood, kidney, liver, skeletal muscle, and heart indicated a rapid incorporation of 14C into several amino acid pools, including alanine, glutamine, glutamate, and aspartate. Four-hour postexercise recoveries (means of CE and IE) of injected [14C]lactate were lactate (0.75%), glucose (0.52%), protein (8.57%), glycogen (18.30%), CO2 (45.18%), and HCO3- (17.72%). Greater (P < 0.05) incorporation of 14C into protein and glycogen constituents after exercise, compared with rest, was demonstrated. Incorporation of [14C]lactate into glycogen represented a significant but only minor fraction of the metabolism of lactate after exhausting exercise. It is suggested that classical explanations of excess postexercise O2 consumption (i.e., "O2 debt") are too simplistic.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                amv
                Archivos de medicina veterinaria
                Arch. med. vet.
                Facultad de Ciencias Veterinarias, Universidad Austral de Chile (Valdivia )
                0301-732X
                2012
                : 44
                : 2
                : 137-144
                Affiliations
                [1 ] Universidad Autónoma Metropolitana México
                [2 ] Universidad Nacional Autónoma de México México
                [3 ] Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán México
                [4 ] Universidad Autónoma de Puebla México
                [5 ] Universidad Nacional Autónoma de México México
                Article
                S0301-732X2012000200007
                10.4067/S0301-732X2012000200007
                98d1d3c8-bad2-4b98-974d-aedb334959b4

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Chile

                Self URI (journal page): http://www.scielo.cl/scielo.php?script=sci_serial&pid=0301-732X&lng=en
                Categories
                VETERINARY SCIENCES

                General veterinary medicine
                dog,gasometry,physiological variables,ejercicio,perro,gasometría,variables fisiológicas,exercise

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