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      Determination of Salivary Cortisol by ELISA and Its Application to the Assessment of the Circadian Rhythm in Children

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          In 35 young children, circadian rhythms of salivary cortisol levels were determined by ELISA using a commercially available kit with a minor modification. The concentration of labeled cortisol in the serum kit was reduced in order to measure cortisol in 10 μl of saliva. Intra- and interassay coefficients of variation for salivary cortisol ranged from 2.4 to 9.9 and 3.2 to 8.9%, respectively. Recovery of salivary cortisol was 82.9-107.0%. There was a highly significant correlation between cortisol levels in saliva and serum in adults (r = 0.857). Salivary cortisol levels ranged from 0.01 to 2.252 μg/l00 ml and showed significant diurnal variation in the children. Our ELISA is a precise, simple, noninvasive and useful method for clinical practice and study in infants and children.

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          Author and article information

          Horm Res Paediatr
          Hormone Research in Paediatrics
          S. Karger AG
          05 December 2008
          : 44
          : 5
          : 213-217
          aDepartment of Maternal and Child Health, Faculty of Medicine, University of Tokyo, bMusashi Hospital, National Center of Neurology and Psychiatry, cDepartment of Obstetrics and Gynecology, Teikyou University School of Medicine, and dSegawa Clinic of Neurology for Children, Tokyo, Japan
          184628 Horm Res 1995;44:213–217
          © 1995 S. Karger AG, Basel

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          Pages: 5
          Original Paper


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