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      Detection of genetic variation in Ocimum species using RAPD and ISSR markers

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          Abstract

          There is a lack of information on the molecular characterization of Ocimum species and hence, efforts have been made under the present study to characterize 17 Ocimum genotypes belonging to 5 different species ( O. basilicum, O. americanum, O. sanctum, O. gratissimum and O. Polystachyon) through random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. PCR amplification using 20 RAPD primers generated a total of 506 loci, of which 490 (96.47 %) loci were found polymorphic. The PIC value for RAPD ranged from 0.907 (OPF 14) to 0.954 (OPC 11) with an average of 0.937. The ISSR primers generated a total of 238 loci, of them 234 (98.17 %) loci were polymorphic. The PIC value ranged from 0.892 (UBC 808) to 0.943 (ISSR A12) with an average of 0.923. The average Jaccard’s similarity coefficient based on RAPD and ISSR analysis was 0.58 and 0.52, respectively. Clustering pattern of dendrogram generated using the pooled RAPD and ISSR data showed all Ocimum genotypes in their respective species groups at a cutoff value of 0.49 and 0.42, respectively. Many unique species-specific alleles were amplified by RAPD and ISSR markers. In both marker systems, a maximum number of unique alleles were observed in O. sanctum. The results of the present investigation provided valid guidelines for collection, conservation and characterization of Ocimum genetic resources.

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          Most cited references27

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          Phytochemical Ecology, Phytochemical Society Symposia Series no. 8

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            Nouvelles rescherches sur la distribution florale

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              Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers

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                Author and article information

                Contributors
                02692-261134 , biotech.aau@gmail.com
                Journal
                3 Biotech
                3 Biotech
                3 Biotech
                Springer Berlin Heidelberg (Berlin/Heidelberg )
                2190-572X
                2190-5738
                9 December 2014
                9 December 2014
                October 2015
                : 5
                : 5
                : 697-707
                Affiliations
                Department of Agricultural Biotechnology, Anand Agricultural University, Anand, 388 110 India
                Article
                269
                10.1007/s13205-014-0269-y
                4569613
                98f5d7bb-b0d4-4b51-a62a-066994c0a484
                © The Author(s) 2014

                Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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                Original Article
                Custom metadata
                © King Abdulaziz City for Science and Technology 2015

                basil, diversity, issr, ocimum, rapd, tulsi

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