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      Community structure of ectomycorrhizal fungi in a Pinus muricata forest: above- and below-ground views

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      Canadian Journal of Botany
      Canadian Science Publishing

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          Ectomycorrhizal fungal succession in jack pine stands following wildfire

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            Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.

            Single-copy sequences can be enzymatically amplified from genomic DNA by the polymerase chain reaction. By using unequal molar amounts of the two amplification primers, it is possible in a single step to amplify a single-copy gene and produce an excess of single-stranded DNA of a chosen strand for direct sequencing or for use as a hybridization probe. Further, individual alleles in a heterozygote can be sequenced directly by using allele-specific oligonucleotides either in the amplification reaction or as sequencing primers. By using these methods, we have studied the allelic diversity at the HLA-DQA locus and its association with the serologically defined HLA-DR and -DQ types. This analysis has revealed a total of eight alleles and three additional haplotypes. This procedure has wide applications in screening for mutations in human genes and facilitates the linking of enzymatic amplification of genes to automated sequencing.
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              Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA

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                Author and article information

                Journal
                Canadian Journal of Botany
                Can. J. Bot.
                Canadian Science Publishing
                0008-4026
                October 1996
                October 1996
                : 74
                : 10
                : 1572-1583
                Article
                10.1139/b96-190
                996316b0-70d5-4f66-ac7a-f9f9a2b9fdf4
                © 1996
                Product
                Self URI (article page): http://www.nrcresearchpress.com/doi/10.1139/b96-190

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