MiR-430 Can Affect the Mesoderm Formation and Metamorphosis of Paralichthys olivaceus by Targeting lefty Gene

Abstract

MiR-430 is a non-coding small RNA that participates in early embryonic development by acting on the Nodal signaling pathway. Its functions in Paralichthys olivaceus ( P. olivaceus), however, remain unclear. In this study, through biological information technology, we hypothesized lefty was the target gene of miR-430. Lefty is a divergent member of the transforming growth factor-β (TGF-β) superfamily, and is involved in regulating left-right patterning as an antagonist of the Nodal signaling pathway. To study the relationship between miR-430 and lefty gene, miR-430 and fluorescent expression vector were transfected into embryo cells. Our results showed that miR-430 has important effects on early embryonic development. It can inhibit the expressions of lefty and other genes related to Nodal signaling pathway. Additionally, the expression of miR-430 was involved in the metamorphosis of P. olivaceus. This study clarifies the function of miR-430 in P. olivaceus, which will be helpful for further research on the interactions between miR-430 and the Nodal signaling pathway.

Journal

Journal ID (publisher-id): JOUC

Title: Journal of Ocean University of China

Publisher: Science Press and Springer (China )

ISSN (Electronic): 1672-5182

Publication date (Electronic): 24 January 2020

Publication date (Print): 01 April 2020

Volume: 19

Issue: 2

Pages: 409-416

Affiliations

¹Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China

Author notes

*Corresponding author: WANG Zhigang, E-mail: zgwang@ 123456ouc.edu.cn

Article

Publisher ID: s11802-020-4131-z

DOI: 10.1007/s11802-020-4131-z

Copyright statement: Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

License:

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Product

Self URI (journal-page): https://www.springer.com/journal/11802