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      ANALYSIS OF TWO POPULATIONS OF THE URUGUAYAN CANINE BREED CIMARRON (Canis familiaris) USING RAPD MARKERS Translated title: ANÁLISIS DE DOS POBLACIONES DE CANINOS (Canis familiaris) DE LA RAZA CIMARRÓN URUGUAYO UTILIZANDO RAPDs COMO MARCADORES MOLECULARES

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          Abstract

          Objective. To analyze the genetic variability in two populations of the native canine breed "Uruguayan Cimarron" from different areas of the country. Materials and methods. DNA was isolated from 40 animals of the Cimarron breed. Using PCR, 11 RAPD (random amplified polymorphism of DNA) molecular markers were analyzed. Results. 14.9% of loci were polymorphic. We found an index of shared bands of 0.91, a genetic distance of 0.16 and a Nei's average diversity index of 0.08. Conclusions. Both populations present a high homogeneity and genetic identity for these markers suggesting that they belong to a common genetic nucleus.

          Translated abstract

          Objetivo. Analizar la variabilidad genética en dos poblaciones de caninos de la raza autóctona "Cimarrón uruguayo" provenientes de distintas zonas del País. Materiales y métodos. Se aisló ADN de 40 animales de la raza Cimarrón. Mediante la técnica de PCR se analizaron 11 marcadores moleculares RAPD (amplificación al azar de ADN polimórfico). Resultados. Se detectó un 14.9% de loci polimórficos. Se obtuvo un índice de bandas compartidas de 0.91, una distancia genética de 0.16 e índice promedio de diversidad de Nei's de 0.08. Conclusiones. Ambas poblaciones presentan una alta homogeneidad e identidad genética para estos marcadores y pertenecerían a un núcleo genético común.

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          Most cited references19

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          A simple and rapid DNA microextraction method for plant, animal, and insect suitable for RAPD and other PCR analyses.

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            Random amplified polymorphic DNA (RAPD) sequences as markers for canine genetic studies

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              Primeros datos de la caracterización etnológica del perro cimarrón

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                Author and article information

                Journal
                mvz
                Revista MVZ Córdoba
                Rev.MVZ Cordoba
                Universidad de Córdoba - Facultad de Medicina Veterinaria y Zootecnia. (Córdoba, Montería, Colombia )
                0122-0268
                1909-0544
                September 2008
                : 13
                : 3
                : 1464-1468
                Affiliations
                [01] Montevideo orgnameUniversity of Uruguay orgdiv1Faculty of Veterinary orgdiv2Laboratory of Genetics Uruguay
                [02] Zaragoza orgnameUniversity of Zaragoza orgdiv1Faculty of Veterinary orgdiv2Laboratory of Cytogenetics and Molecular Genetics Spain
                Article
                S0122-02682008000300005 S0122-0268(08)01300305
                99a73961-2255-41e3-938c-fa68ebc4c897

                This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

                History
                : 05 May 2008
                : 10 December 2008
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 12, Pages: 5
                Product

                SciELO Colombia

                Categories
                Originals

                Uruguay,genome,Canis familiaris,genoma
                Uruguay, genome, Canis familiaris, genoma

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