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      First report and new molecular and morphological characterizations of root-knot nematode, Meloidogyne javanica, infecting ginger and long coriander in Vietnam


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          Ginger ( Zingiber officinale Roscoe) and long coriander ( Eryngium foetidum L.) are commonly grown and used as important spices and medicinal plants in Vietnam. Our study recovered for the first time one of the most damaging tropical root-knot nematodes, Meloidogyne javanica, associated with these plants in the Western Highlands of Vietnam. In this study, M. javanica was characterized based on morphology and molecular characterization of D2-D3 fragment of 28S rRNA, ITS, and Nad5 mtDNA regions. The identification of this species was done based on a combination of morphology, multiplex-PCR with specific primer, network haplotype analysis, and PPNID program.

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          popart: full-feature software for haplotype network construction

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            Top 10 plant-parasitic nematodes in molecular plant pathology.

            The aim of this review was to undertake a survey of researchers working with plant-parasitic nematodes in order to determine a 'top 10' list of these pathogens based on scientific and economic importance. Any such list will not be definitive as economic importance will vary depending on the region of the world in which a researcher is based. However, care was taken to include researchers from as many parts of the world as possible when carrying out the survey. The top 10 list emerging from the survey is composed of: (1) root-knot nematodes (Meloidogyne spp.); (2) cyst nematodes (Heterodera and Globodera spp.); (3) root lesion nematodes (Pratylenchus spp.); (4) the burrowing nematode Radopholus similis; (5) Ditylenchus dipsaci; (6) the pine wilt nematode Bursaphelenchus xylophilus; (7) the reniform nematode Rotylenchulus reniformis; (8) Xiphinema index (the only virus vector nematode to make the list); (9) Nacobbus aberrans; and (10) Aphelenchoides besseyi. The biology of each nematode (or nematode group) is reviewed briefly. © 2013 BSPP AND JOHN WILEY & SONS LTD.
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              Median-joining networks for inferring intraspecific phylogenies.

              Reconstructing phylogenies from intraspecific data (such as human mitochondrial DNA variation) is often a challenging task because of large sample sizes and small genetic distances between individuals. The resulting multitude of plausible trees is best expressed by a network which displays alternative potential evolutionary paths in the form of cycles. We present a method ("median joining" [MJ]) for constructing networks from recombination-free population data that combines features of Kruskal's algorithm for finding minimum spanning trees by favoring short connections, and Farris's maximum-parsimony (MP) heuristic algorithm, which sequentially adds new vertices called "median vectors", except that our MJ method does not resolve ties. The MJ method is hence closely related to the earlier approach of Foulds, Hendy, and Penny for estimating MP trees but can be adjusted to the level of homoplasy by setting a parameter epsilon. Unlike our earlier reduced median (RM) network method, MJ is applicable to multistate characters (e.g., amino acid sequences). An additional feature is the speed of the implemented algorithm: a sample of 800 worldwide mtDNA hypervariable segment I sequences requires less than 3 h on a Pentium 120 PC. The MJ method is demonstrated on a Tibetan mitochondrial DNA RFLP data set.

                Author and article information

                J Nematol
                J Nematol
                Journal of Nematology
                Exeley Inc.
                15 February 2021
                : 53
                : e2021-11
                [1 ]Vietnam National Museum of Nature , Vietnam Academy of Sciences and Technology , 18 Hoang Quoc Viet, Cau Giay, 100000, Hanoi, Vietnam
                [2 ]Institute of Ecology and Biological Resources , Vietnam Academy of Sciences and Technology , 18 Hoang Quoc Viet, Cau Giay, 100000, Hanoi, Vietnam
                [3 ]Graduate University of Science and Technology , Vietnam Academy of Sciences and Technology , 18 Hoang Quoc Viet, Cau Giay, 100000, Hanoi, Vietnam
                [4 ]Nematology Research Unit , Department of Biology , Ghent University , K.L. Ledeganckstraat 35, 9000, Ghent, Belgium
                Author notes

                This paper was edited by Zafar Ahmad Handoo.

                © 2021 Authors

                This is an Open Access article licensed under the Creative Commons CC BY 4.0 license, https://creativecommons.org/licenses/by/4.0/

                : 16 December 2020
                Arts & Humanities

                28s rrna,its,mtdna,dna barcode,multiplex-pcr,taxonomy,plant-parasitic nematodes


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