+1 Recommend
1 collections
      • Record: found
      • Abstract: found
      • Article: found

      Endothelial Loss due to Leukocytes in Canine Experimental Vein-to-Artery Grafts


      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          Vein-to-artery grafts develop areas of endothelial loss with fibrin and leukocytes which lead to early thrombosis and may lead to subsequent atherosclerosis of the graft. En face monolayers were prepared which removed >90% of vascular intima. Unevenly distributed leukocytes and endothelial cells were counted using a standardized sampling of calibrated oil immersion fields of 0.01 mm2. Nongrafted veins had 14 ± 1 evenly arranged endothelial cells per field without gaps or leukocytes, while 10-min grafts had 13 ± 2 with rare leukocytes. Four-hour grafts from normal dogs had 9 ± 2 endothelial cells with gaps and 97 ± 37 neutrophils and 44 ± 25 monocytes. Leukopenic dogs (vinblastine-treated) had normal numbers of endothelial cells (14 ± 1) with scanty leukocytes. We conclude that leukocytes cause endothelial loss in vein-to-artery grafts that can be prevented by intense leukopenia. This may lead to practical approaches to protecting such grafts in humans.

          Related collections

          Author and article information

          J Vasc Res
          Journal of Vascular Research
          S. Karger AG
          23 September 2008
          : 23
          : 4-5
          : 173-182
          Departments of Medicine and Surgery, New York Medical College, Westchester County Medical Center, Valhalla, N.Y., USA
          158640 Blood Vessels 1986;23:173–182
          © 1986 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          : 15 July 1985
          : 06 February 1986
          Page count
          Pages: 10
          Research Paper

          General medicine,Neurology,Cardiovascular Medicine,Internal medicine,Nephrology
          Endothelium,Graft, vein-to-artery,Monocyte,Neutrophil,Leukocyte,Leukopenia


          Comment on this article