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      A Rapid MALDI-TOF MS Identification Database at Genospecies Level for Clinical and Environmental Aeromonas Strains

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          Abstract

          The genus Aeromonas has undergone a number of taxonomic and nomenclature revisions over the past 20 years, and new (sub)species and biogroups are continuously described. Standard identification methods such as biochemical characterization have deficiencies and do not allow clarification of the taxonomic position. This report describes the development of a matrix-assisted laser desorption/ionisation–time of flight mass spectrometry (MALDI-TOF MS) identification database for a rapid identification of clinical and environmental Aeromonas isolates.

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          Applications of whole-cell matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry in systematic microbiology.

          Martin Welker, Edward R.B. Moore (2011)
          In the last few years matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been increasingly studied and applied for the identification and typing of microorganisms. Very recently, MALDI-TOF MS has been introduced in clinical routine microbiological diagnostics with marked success, which is remarkable considering that not long ago the technology was generally seen as being far from practical application. The identification of microbial isolates by whole-cell mass spectrometry (WC-MS) is being recognized as one of the latest tools forging a revolution in microbial diagnostics, with the potential of bringing to an end many of the time-consuming and man-power-intensive identification procedures that have been used for decades. Apart from applications of WC-MS in clinical diagnostics, other fields of microbiology also have adopted the technology with success. In this article, an over-view of the principles of MALDI-TOF MS and WC-MS is presented, highlighting the characteristics of the technology that allow its utilization for systematic microbiology. Copyright © 2010 Elsevier GmbH. All rights reserved.
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            Evolving concepts regarding the genus Aeromonas: an expanding Panorama of species, disease presentations, and unanswered questions.

            Benjamin Abbott, J. Janda (1998)
            It has been almost 10 years since a major review on the association of Aeromonas with human disease has been published. During that period the number of valid species in the genus has grown to 14, with a new family (Aeromonadaceae) established to house this genus. Despite this explosion in the number of new genomospecies, only five (Aeromonas hydrophila, A. caviae, A. veronii, A. jandaei, and A. schubertii) are currently recognized as human pathogens. New syndromes attributed to this genus include hemolytic uremic syndrome, burn-associated sepsis, and a variety of respiratory tract infections, including epiglottitis. Convincing evidence suggests that some aeromonads do cause gastroenteritis, but it is presently unclear whether many of the strains isolated from feces are involved in diarrheal disease. Many questions regarding this genus remain unanswered.
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              Characterization of intact microorganisms by MALDI mass spectrometry.

              C Fenselau, P A Demirev (2015)
              The application of MALDI mass spectrometry to desorb protein biomarkers from intact viruses, bacteria, fungus, and spores is the focus of this review. Instrumentation, sample collection, sample preparation, and algorithms for data analysis are summarized. Optimally these analyses should be carried out in less than five minutes. Successful applications are discussed from biotechnology, cell biology, and the pharmaceutical industry. Copyright 2002 John Wiley & Sons, Inc.
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                Author and article information

                Contributors
                Mikael Skurnik: Role: Editor
                Journal
                Journal ID (nlm-ta): PLoS One
                Journal ID (iso-abbrev): PLoS ONE
                Journal ID (publisher-id): plos
                Journal ID (pmc): plosone
                Title: PLoS ONE
                Publisher: Public Library of Science (San Francisco, USA )
                ISSN (Electronic): 1932-6203
                Publication date Collection: 2012
                Publication date (Electronic): 31 October 2012
                Volume: 7
                Issue: 10
                Electronic Location Identifier: e48441
                Affiliations
                [1 ]Institute of Microbiology, Bellinzona, Switzerland
                [2 ]Mabritec AG, Riehen, Switzerland
                [3 ]Microbial Ecology, Microbiology Unit, Plant Biology Department University of Geneva, Genève, Switzerland
                University of Helsinki, Finland
                Author notes

                Competing Interests: The authors have read the journal’s policy and have the following conflicts: Dominik Ziegler is employed by Mabritec AG. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials.

                Conceived and designed the experiments: CB AD OP MT. Performed the experiments: CB. Analyzed the data: CB APC DZ MT. Contributed reagents/materials/analysis tools: OP. Wrote the paper: CB OP.

                Article
                Publisher ID: PONE-D-12-13637
                DOI: 10.1371/journal.pone.0048441
                PMC ID: 3485216
                PubMed ID: 23119019
                SO-VID: 9ac46a82-b621-49f4-8f82-173134ad2d5d
                Copyright statement: Copyright @ 2012
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                Date received : 10 May 2012
                Date accepted : 25 September 2012
                Page count
                Pages: 6
                Funding
                No current external funding sources for this study.
                Categories
                Subject: Research Article
                Subject: Biology
                Subject: Evolutionary Biology
                Subject: Evolutionary Systematics
                Subject: Taxonomy
                Subject: Microbial Taxonomy
                Subject: Microbiology
                Subject: Bacterial Pathogens
                Subject: Gram Negative
                Subject: Bacteriology
                Subject: Bacterial Taxonomy
                Subject: Medical Microbiology
                Subject: Microbial Ecology
                Subject: Microbial Pathogens
                Subject: Proteomics
                Subject: Proteomic Databases
                Subject: Sequence Analysis
                Subject: Sequencing
                Subject: Spectrometric Identification of Proteins

                ScienceOpen disciplines: Uncategorized
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                ScienceOpen disciplines: Uncategorized

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