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      Label-free Electrochemical Impedance Spectroscopy Aptasensor for Ultrasensitive Detection of Lung Cancer Biomarker Carcinoembryonic Antigen

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          Abstract

          In this work, an integrated electrode system consisting of a graphene working electrode, a carbon counter electrode and an Ag/AgCl reference electrode was fabricated on an FR-4 glass fiber plate by a polyethylene self-adhesive mask stencil method combined with a manual screen printing technique. The integrated graphene electrode was used as the base electrode, and AuNPs were deposited on the working electrode surface by cyclic voltammetry. Then, the carcinoembryonic antigen aptamer was immobilized using the sulfhydryl self-assembly technique. The sensor uses [Fe(CN) 6] 3−/4− as a redox probe for label free detection of carcinoembryonic antigen based on the impedance change caused by the difference in electron transfer rate before and after the binding of carcinoembryonic antigen aptamer and the target carcinoembryonic antigen. The results showed a good linear relationship when the CEA concentration is in the range of 0.2–15.0 ng/ml. The detection limit was calculated to be 0.085 ng/ml (S/N = 3).

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          Current approaches in SELEX: An update to aptamer selection technology.

          Systematic evolution of ligands by exponential enrichment (SELEX) is a well-established and efficient technology for the generation of oligonucleotides with a high target affinity. These SELEX-derived single stranded DNA and RNA molecules, called aptamers, were selected against various targets, such as proteins, cells, microorganisms, chemical compounds etc. They have a great potential in the use as novel antibodies, in cancer theragnostics and in biomedical research. Vast interest in aptamers stimulated continuous development of SELEX, which underwent numerous modifications since its first application in 1990. Novel modifications made the selection process more efficient, cost-effective and significantly less time-consuming. This article brings a comprehensive and up-to-date review of recent advances in SELEX methods and pinpoints advantages, main obstacles and limitations. The post-SELEX strategies and examples of application are also briefly outlined in this review.
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            A critical review on the use of potentiometric based biosensors for biomarkers detection

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              Tuning of metal oxides photocatalytic performance using Ag nanoparticles integration

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                Author and article information

                Contributors
                Journal
                Front Chem
                Front Chem
                Front. Chem.
                Frontiers in Chemistry
                Frontiers Media S.A.
                2296-2646
                19 July 2021
                2021
                : 9
                : 721008
                Affiliations
                [ 1 ]Department of Medical Oncology, Qilu Hospital, Shandong University, Jinan, China
                [ 2 ]Shandong Academy of Pharmaceutical Sciences, Jinan, China
                Author notes

                Edited by: Fatemeh Karimi, Quchan University of Advanced Technology, Iran

                Reviewed by: Somaye Cheraghi, Shahid Bahonar University of Kerman, Iran

                Vahid Arabali, Islamic Azad University Sari Branch, Iran

                *Correspondence: Xiuwen Wang, xiuwenwang@ 123456vtcuni.com

                This article was submitted to Electrochemistry, a section of the journal Frontiers in Chemistry

                Article
                721008
                10.3389/fchem.2021.721008
                8326396
                34350159
                9b12adcd-fe73-4047-adb0-c062a8668727
                Copyright © 2021 Wang, Chen, Xuan, Wang and Wang.

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                History
                : 05 June 2021
                : 07 July 2021
                Categories
                Chemistry
                Original Research

                screen-printed carbon electrode,aptamer,carcinoembryonic antigen,eis aptamer sensor,graphene nano-sheet

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