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      Influence of fast-food products on liver element composition and metabolic parameters of laboratory animals

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          Abstract

          Abstract. Objective: The aim of the work was to study the influence of fast-food and instant-food products on metabolic indicators and element status of laboratory animals. Materials and methods: The study was conducted on male Wistar rats. The experimental group consumed a semi-synthetic diet consisting of mixture of basic diet (50%), fast-food and instant food (50%). Total protein, ALT, AST, total bilirubin and triiodothyronine levels were assessed. The element composition of the liver was determined by atomic-emission and mass-spectrometry with inductively-coupled argon plasma. The activities of Cu, Zn-superoxide dismutase, and glutathione peroxidase were measured in erythrocyte lysate by spectrophotometric method. Results: A significant decrease in the content of a number of essential elements: Mn (3.6-fold), Se (1.21-fold), Si (3.6-fold), and Zn (1.25-fold) was observed. Al and Cd contents in the experimental group were significantly increased by a factor of 3.9 and 2, respectively. Consumption of fast-food products was accompanied by significantly lower values of total protein and greater activity of AST and ALT in serum. Activity of glutathione peroxidase (140.5 (121.0 – 151.0) U/mL) was significantly decreased (р = 0.000) in the experimental group in comparison to the control group (240.0 (229.0 – 251.0) U/mL).


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          Characterization of silver nanoparticles synthesized by using marine isolate Streptomyces albidoflavus.

          Silver nanoparticles production by the green chemistry approach was investigated using an isolated marine actinomycetes strain. The isolated strain was identified as Streptomyces albidoflavus based on chemotaxonomic and ribotyping properties. The strain revealed production of silver nanoparticles both extracellular and intracellularly. Surface Plasmon Resonance analysis with the function of time revealed that particle synthesis by this strain is reaction time dependent. The produced particles were spherical shaped and monodispersive in nature and showed a single surface plasmon resonance peak at 410 nm. Size distribution histograms indicated production of 10-40- nm-size nanoparticles with a mean size of 14.5 nm. FT-IR spectra of nanopartilces showed N-H, C-H, and C-N stretching vibrations, denoting the presence of amino acid/ peptide compounds on the surface of silver nanoparticles produced by S. albidoflavus. Synthesized nanoparticles revealed a mean negative zeta potential and electrophoretic mobility of -8.5 mV and -0.000066 cm2/Vs, respectively. The nanoparticles produced were proteinaceous compounds as capping agents with -8.5 mV zeta potential and revealed antimicrobial activity against both Gram-negative and -positive bacterial strains. Owing to their small size, these particles have greater impact on industrial application spectra.
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            Tissue trace elements and lipid peroxidation in breeding female bank voles Myodes glareolus.

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              Author and article information

              Journal
              Trace Elements and Electrolytes
              TE
              Dustri-Verlgag Dr. Karl Feistle
              0946-2104
              2018
              October 01 2018
              : 35
              : 10
              : 228-231
              Article
              10.5414/TEX0155415
              9b3704a1-c8b1-42c8-8816-1904971e64e6
              © 2018
              History

              Endocrinology & Diabetes,General medicine,Medicine,Gastroenterology & Hepatology,Nutrition & Dietetics
              trace elements,fast food,instant food,­antioxidant enzymes

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