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      Draft Genome Sequence of Ureolytic Environmental Isolate Bacillus galactosidilyticus PL133

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          Abstract

          We report here the 5.19-Mb draft genome sequence of Bacillus galactosidilyticus PL133 isolated from poultry litter. The isolate was an important member of the cultivable aerobic bacteria identified to have ureolytic activity, which is responsible for ammonia generation in poultry litter residue.

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          Development of a quantitative real-time polymerase chain reaction assay to target a novel group of ammonia-producing bacteria found in poultry litter.

          Ammonia production in poultry houses has serious implications for flock health and performance, nutrient value of poultry litter, and energy costs for running poultry operations. In poultry litter, the conversion of organic N (uric acid and urea) to NH(4)-N is a microbially mediated process. The urease enzyme is responsible for the final step in the conversion of urea to NH(4)-N. Cloning and analysis of 168 urease sequences from extracted genomic DNA from poultry litter samples revealed the presence of a novel, dominant group of ureolytic microbes (representing 90% of the urease clone library). Specific primers and a probe were designed to target this novel poultry litter urease producer (PLUP) group, and a new quantitative real-time PCR assay was developed. The assay allowed for the detection of 10(2) copies of target urease sequences per PCR reaction (approximately 1 x 10(4) cells per gram of poultry litter), and the reaction was linear over 8 orders of magnitude. Our PLUP group was present only in poultry litter and was not present in environmental samples from diverse agricultural settings. This novel PLUP group represented between 0.1 to 3.1% of the total microbial populations (6.0 x 10(6) to 2.4 x 10(8) PLUP cells per gram of litter) from diverse poultry litter types. The PLUP cell concentrations were directly correlated to the total cell concentrations in the poultry litter and were found to be influenced by the physical parameters of the litters (bedding material, moisture content, pH), as well as the NH(4)-N content of the litters, based on principal component analysis. Chemical parameters (organic N, total N, total C) were not found to be influential in the concentrations of our PLUP group in the diverse poultry litters Future applications of this assay could include determining the efficacy of current NH(4)-N-reducing litter amendments or in designing more efficient treatment protocols.
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            Author and article information

            Journal
            Genome Announc
            Genome Announc
            ga
            ga
            GA
            Genome Announcements
            American Society for Microbiology (1752 N St., N.W., Washington, DC )
            2169-8287
            6 October 2016
            Sep-Oct 2016
            : 4
            : 5
            : e01067-16
            Affiliations
            School of Environmental Sciences, University of Guelph, Guelph, Ontario, Canada
            Author notes
            Address correspondence to Marc Habash, mhabash@ 123456uoguelph.ca .
            Article
            genomeA01067-16
            10.1128/genomeA.01067-16
            5054316
            9b3fb3b7-4364-4ca4-ab32-6d334af552cf
            Copyright © 2016 Gaiero et al.

            This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

            History
            : 12 August 2016
            : 17 August 2016
            Page count
            Figures: 0, Tables: 0, Equations: 0, References: 3, Pages: 1, Words: 915
            Funding
            Funded by: Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (NSERC) http://dx.doi.org/10.13039/501100000038
            Award Recipient : Marc Habash
            Funded by: Gouvernement du Canada | Agriculture and Agri-Food Canada (AAFC) http://dx.doi.org/10.13039/501100000040
            Award Recipient : Rob W. Nicol
            The CanAdvance program is funded by Agriculture and Agri-Food Canada (AAFC).
            Categories
            Prokaryotes
            Custom metadata
            September/October 2016

            Genetics
            Genetics

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