12
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Translationally controlled tumour protein (TCTP) from tomato and Nicotiana benthamiana is necessary for successful infection by a potyvirus

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Summary

          Translationally controlled tumour protein (TCTP) is a ubiquitously distributed protein in eukaryotes, involved in the regulation of several processes, including cell cycle progression, cell growth, stress protection, apoptosis and maintenance of genomic integrity. Its expression is induced during the early stages of tomato ( Solanum lycopersicum) infection by the potyvirus Pepper yellow mosaic virus (PepYMV, a close relative of Potato virus Y). Tomato TCTP is a protein of 168 amino acids, which contains all the conserved domains of the TCTP family. To study the effects of TCTP silencing in PepYMV infection, Nicotiana benthamiana plants were silenced by virus‐induced gene silencing (VIGS) and transgenic tomato plants silenced for TCTP were obtained. In the early stages of infection, both tomato and N. benthamiana silenced plants accumulated less virus than control plants. Transgenic tomato plants showed a drastic reduction in symptoms and no viral accumulation at 14 days post‐inoculation. Subcellular localization of TCTP was determined in healthy and systemically infected N. benthamiana leaves. TCTP was observed in both the nuclei and cytoplasm of non‐infected cells, but only in the cytoplasm of infected cells. Our results indicate that TCTP is a growth regulator necessary for successful PepYMV infection and that its localization is altered by the virus, probably to favour the establishment of virus infection. A network with putative interactions that may occur between TCTP and Arabidopsis thaliana proteins was built. This network brings together experimental data of interactions that occur in other eukaryotes and helps us to discuss the possibilities of TCTP involvement in viral infection.

          Related collections

          Author and article information

          Contributors
          palfenas@ufv.br
          Journal
          Mol Plant Pathol
          Mol. Plant Pathol
          10.1111/(ISSN)1364-3703
          MPP
          Molecular Plant Pathology
          John Wiley and Sons Inc. (Hoboken )
          1464-6722
          1364-3703
          27 July 2016
          June 2017
          : 18
          : 5 ( doiID: 10.1111/mpp.2017.18.issue-5 )
          : 672-683
          Affiliations
          [ 1 ] Departamento de Microbiologia/BIOAGRO/National Institute of Science and Technology in Plant‐Pest Interactions Universidade Federal de Viçosa Viçosa MG 36570‐900 Brazil
          [ 2 ] Departamento de Fitopatologia/BIOAGRO/National Institute of Science and Technology in Plant‐Pest Interactions Universidade Federal de Viçosa Viçosa MG 36570‐900 Brazil
          [ 3 ] Departamento de Biologia Vegetal/BIOAGRO Universidade Federal de Viçosa Viçosa MG 36570‐900 Brazil
          Author notes
          [*] [* ] Correspondence: Email: palfenas@ 123456ufv.br
          Article
          PMC6638207 PMC6638207 6638207 MPP12426
          10.1111/mpp.12426
          6638207
          27159273
          9b960687-211c-4c8c-95e6-d0b75f9f9c4a
          © 2016 BSPP AND JOHN WILEY & SONS LTD
          History
          : 08 March 2016
          : 02 May 2016
          : 05 May 2016
          Page count
          Figures: 8, Tables: 0, Pages: 12, Words: 8623
          Funding
          Funded by: Conselho Nacional de Desenvolvimento Científico e Tecnológico
          Award ID: 478633/2008‐4
          Award ID: 482631/2010‐4
          Funded by: International Foundation for Science
          Award ID: C/4978‐1
          Categories
          Original Article
          Original Articles
          Custom metadata
          2.0
          mpp12426
          June 2017
          Converter:WILEY_ML3GV2_TO_NLMPMC version:5.6.4 mode:remove_FC converted:10.06.2019

          potyvirus, Pepper yellow mosaic virus ,translationally controlled tumour protein,host factor

          Comments

          Comment on this article