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      Subcellular Distribution of Corticotropin-Releasing-Factor-Like Immunoreactivity in Rat Central Nervous System

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          Abstract

          Corticotropin-releasing-factor-like immunoreactivity (CRF-LI) was measured in a number of subcellular fractions from rat brain using a highly sensitive and specific radioimmunoassay. CRF-LI was highly enriched in the crude synaptosomal/ mitochondrial fraction (P<sub>2</sub>) relative to the homogenate, P<sub>1</sub>, S<sub>1</sub>, and S<sub>2</sub> fractions. Separation of the P<sub>2</sub> fraction into synaptosomal, myelin, and mitochondria-enriched subfractions on a rapid one-step sucrose gradient revealed that CRF-LI was present at higher concentrations in the synaptosomal fraction than in the mitochondrial and myelin fractions. The distribution of CRF-LI paralleled that of synapsin, a synaptic vesicle marker phosphoprotein, but not that of pyruvate dehydrogenase, a mitochondrial phosphoprotein. These results are consistent with a nerve terminal localization of CRF and a potential role for this peptide as a central nervous system neurotransmitter.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1991
          1991
          04 April 2008
          : 54
          : 1
          : 36-41
          Affiliations
          Departments of aPsychiatry and bPharmacology, Duke University Medical Center, Durham, N.C., USA
          Article
          125848 Neuroendocrinology 1991;54:36–41
          10.1159/000125848
          1922675
          © 1991 S. Karger AG, Basel

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          Page count
          Pages: 6
          Categories
          Original Paper

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