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      Two-Component Systems Involved in Susceptibility to Nisin A in Streptococcus pyogenes

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          ABSTRACT

          Two-component systems (TCSs) are regulatory systems in bacteria that play important roles in sensing and adapting to the environment. In this study, we systematically evaluated the roles of TCSs in the susceptibility of the group A Streptococcus (GAS; Streptococcus pyogenes) SF370 strain to several types of lantibiotics. Using individual TCS deletion mutants, we found that the deletion of srtRK (spy_1081–spy_1082) in SF370 increased the susceptibility to nisin A, which is produced by Lactococcus lactis ATCC 11454, but susceptibility to other types of lantibiotics (nukacin ISK-1, produced by Staphylococcus warneri, and staphylococcin C55, produced by Staphylococcus aureus) was not altered in the TCS mutants tested. The expression of srtFEG (spy_1085 to spy_1087), which is located downstream of srtRK and is homologous to ABC transporters, was increased in response to nisin A. However, srtEFG expression was not induced by nisin A in the srtRK mutant. The inactivation of srtFEG increased the susceptibility to nisin A. These results suggest that SrtRK controls SrtFEG expression to alter the susceptibility to nisin A. Further experiments showed that SrtRK is required for coexistence with L. lactis ATCC 11454, which produces nisin A. Our results elucidate the important roles of S. pyogenes TCSs in the interactions between different bacterial species, including bacteriocin-producing bacteria.

          IMPORTANCE In this study, we focused on the association of TCSs with susceptibility to bacteriocins in S. pyogenes SF370, which has no ability to produce bacteriocins, and reported two major new findings. We demonstrated that the SrtRK TCS is related to susceptibility to nisin A by controlling the ABC transporter SrtFEG. We also showed that S. pyogenes SrtRK is important for survival when the bacteria are cocultured with nisin A-producing Lactococcus lactis. This report highlights the roles of TCSs in the colocalization of bacteriocin-producing bacteria and non-bacteriocin-producing bacteria. Our findings provide new insights into the function of TCSs in S. pyogenes.

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          Author and article information

          Contributors
          Role: Editor
          Journal
          Appl Environ Microbiol
          Appl. Environ. Microbiol
          aem
          aem
          AEM
          Applied and Environmental Microbiology
          American Society for Microbiology (1752 N St., N.W., Washington, DC )
          0099-2240
          1098-5336
          29 July 2016
          16 September 2016
          1 October 2016
          : 82
          : 19
          : 5930-5939
          Affiliations
          [a ]Department of Oral Microbiology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
          [b ]Department of Bacteriology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
          [c ]Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
          [d ]Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
          [e ]Research and Education Center for Advanced Oral Science, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
          INRS–Institut Armand-Frappier
          Author notes
          Address correspondence to Hitoshi Komatsuzawa, hkomatsu@ 123456dent.kagoshima-u.ac.jp .

          Citation Kawada-Matsuo M, Tatsuno I, Arii K, Zendo T, Oogai Y, Noguchi K, Hasegawa T, Sonomoto K, Komatsuzawa H. 2016. Two-component systems involved in susceptibility to nisin A in Streptococcus pyogenes. Appl Environ Microbiol 82:5930–5939. doi: 10.1128/AEM.01897-16.

          Article
          PMC5038018 PMC5038018 5038018 01897-16
          10.1128/AEM.01897-16
          5038018
          27474716
          9c58f3dc-9681-4caf-9afa-41e6fa9bffc2
          Copyright © 2016, American Society for Microbiology. All Rights Reserved.
          History
          : 22 June 2016
          : 13 July 2016
          Page count
          Figures: 8, Tables: 3, Equations: 0, References: 35, Pages: 10, Words: 7528
          Funding
          Funded by: Ministry of Education, Culture, Sports, Science, and Technology (MEXT) http://dx.doi.org/10.13039/501100001700
          Award ID: 15K11017
          Award Recipient : Miki Kawada-Matsuo
          Categories
          Environmental Microbiology

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