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      A pause site for RNA polymerase II is associated with termination of transcription.

      The EMBO Journal
      Base Sequence, Gene Expression Regulation, Globins, genetics, HeLa Cells, Humans, Introns, Molecular Sequence Data, Poly A, RNA Polymerase II, metabolism, Terminator Regions, Genetic, Transcription, Genetic

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          Abstract

          Termination of transcription by RNA polymerase II has been postulated to involve a pausing process. We have identified such a pause signal, 350 bp into the 3' flanking region of the human alpha 2 globin gene at a position where termination is thought to occur. We show that this pause signal enhances the utilization of an upstream poly(A) site which is otherwise out-competed by a stronger downstream poly(A) site. We also demonstrate that the pause site rescues a poly(A) site that is inactive due to its location within an intron. Using nuclear run-on analysis we show that elongating RNA polymerase II molecules accumulate over this pause signal. Furthermore we show that when the pause site is positioned immediately downstream of a strong poly(A) signal, significant levels of transcription termination take place.

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          Author and article information

          Journal
          2050120
          452858
          10.1002/j.1460-2075.1991.tb07709.x

          Chemistry
          Base Sequence,Gene Expression Regulation,Globins,genetics,HeLa Cells,Humans,Introns,Molecular Sequence Data,Poly A,RNA Polymerase II,metabolism,Terminator Regions, Genetic,Transcription, Genetic

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