12
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Quantitative determination of chromophore concentrations from 2D photoacoustic images using a nonlinear model-based inversion scheme.

      Applied optics
      Algorithms, Biopolymers, analysis, Computer Simulation, Image Interpretation, Computer-Assisted, methods, Microscopy, Acoustic, Models, Biological, Reproducibility of Results, Sensitivity and Specificity

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          A model-based inversion scheme was used to determine absolute chromophore concentrations from multiwavelength photoacoustic images. The inversion scheme incorporated a forward model, which predicted 2D images of the initial pressure distribution as a function of the spatial distribution of the chromophore concentrations. It comprised a multiwavelength diffusion based model of the light transport, a model of acoustic propagation and detection, and an image reconstruction algorithm. The model was inverted by fitting its output to measured photoacoustic images to determine the chromophore concentrations. The scheme was validated using images acquired in a tissue phantom at wavelengths between 590 nm and 980 nm. The phantom comprised a scattering emulsion in which up to four tubes, filled with absorbing solutions of copper and nickel chloride at different concentration ratios, were submerged. Photoacoustic signals were detected along a line perpendicular to the tubes from which images of the initial pressure distribution were reconstructed. By varying the excitation wavelength, sets of multiwavelength photoacoustic images were obtained. The majority of the determined chromophore concentrations were within +/-15% of the true value, while the concentration ratios were determined with an average accuracy of -1.2%.

          Related collections

          Author and article information

          Comments

          Comment on this article