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      Retraction Note: The effects of dexamethasone on the proliferation and apoptosis of human ovarian cancer cells induced by paclitaxel

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          Abstract

          Retraction The Publisher and Editor regretfully retract this article [1] because the peer-review process was inappropriately influenced and compromised. As a result, the scientific integrity of the article cannot be guaranteed. A systematic and detailed investigation suggests that a third party was involved in supplying fabricated details of potential peer reviewers for a large number of manuscripts submitted to different journals. In accordance with recommendations from COPE we have retracted all affected published articles, including this one. It was not possible to determine beyond doubt that the authors of this particular article were aware of any third party attempts to manipulate peer review of their manuscript.

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          The effects of dexamethasone on the proliferation and apoptosis of human ovarian cancer cells induced by paclitaxel

          Background Dexamethasone (DEX) has been routinely used as a pre-treatment in the clinical application of paclitaxel (PTX) to treat ovarian cancer. However, PTX-induced apoptosis might be inhibited by DEX. This study was undertaken to investigate the effects of DEX on the apoptosis induced by PTX. Methods Both of SKOV-3 and HO-8910 human ovarian cancer cells were divided into four groups: (1) untreated (Con); (2) treated with DEX (0.1 μM) alone; (3) treated with PTX (50 nM); and (4) pre-treated with DEX (0.1 μM), and 24 h later, treated with PTX (DEX + PTX). Cell proliferation was determined by the 3-(4,5)-dimethylthiahiazo (−z-y1)-3,5-di- phenytetrazoliumromide (MTT) dye uptake method, while cell apoptosis was analyzed by propidium iodide (PI) staining and flow cytometry. Then, reverse transcription polymerase chain reactions (RT-PCRs) were applied to semi-quantitative analysis, followed by western blot analysis. Statistical analysis was performed, with Fisher’s least significant difference test. Results Our results demonstrated that DEX can differentially inhibit SKOV-3 and HO-8910 cell proliferation induced by PTX and decrease the apoptosis rates in cancer cells. Pre-treatment with DEX could up-regulate the expressions of members of anti-apoptotic Bcl-2 family (Bcl-2 and Bcl-XL) and members of IAP family (survivin). The expression of cleaved caspase-3 was down-regulated by DEX, shown by semi-quantitative RT-PCRs and western blot analysis. Conclusions Our data gained invaluable insights of the antagonistic mechanisms of DEX on PTX-induced cancer cell death and may provide new methods of using DEX as antineoplastic drugs or agents in the clinical treatment for ovarian cancer patients.
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            Author and article information

            Contributors
            rongzhangrz@hotmail.com
            rongzhangrz@hotmail.com
            rongzhangrz@hotmail.com
            rongzhangrz@hotmail.com
            rongzhangrz@hotmail.com
            rongzhangrz@hotmail.com
            Journal
            J Ovarian Res
            J Ovarian Res
            Journal of Ovarian Research
            BioMed Central (London )
            1757-2215
            26 March 2015
            26 March 2015
            2015
            : 8
            : 16
            Affiliations
            Department of Obstetrics and Gynecology, Shanghai Jiaotong University-Affiliated Sixth People’s Hospital of Fengxian Branch, 6600 Nanfeng Road, Shanghai, 201499 People’s Republic of China
            Article
            144
            10.1186/s13048-015-0144-4
            4374203
            9d4c1392-f2ae-4baa-82c5-4482a691bb0b
            © Hou et al.; licensee BioMed Central. 2015

            This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

            History
            : 26 January 2015
            : 27 February 2015
            Categories
            Retraction Note
            Custom metadata
            © The Author(s) 2015

            Obstetrics & Gynecology
            Obstetrics & Gynecology

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