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      Isolation, purification and chemical characterization of a new angucyclinone compound produced by a new halotolerant Nocardiopsis sp. HR-4 strain.

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          Abstract

          A halotolerant Actinobacteria strain HR-4 was isolated from a salt lake soil sample in Algerian Sahara. Analysis of 16S rDNA gene sequence showed that strain HR-4 belonged to the genus Nocardiopsis. The similarity level ranges between 97.45 and 99.20% with Nocardiopsis species and Nocardiopsis rosea being the most closely related one. Morphological, physiological and phylogenetic characteristics comparisons showed significant differences with the nearest species. These data strongly suggest that strain HR-4 represents novel species. The antimicrobial activity of strain HR-4 showed an antibacterial activity against Gram-positive bacteria as well as an antifungal one. Two major natural products including a new one were isolated from the culture broth using various separation and purification procedures. The chemical structure established on the basis of spectroscopic studies NMR and by comparing with spectroscopic data from the literature of the two compounds affirm that they are classified in the group of Angucyclinones. This is the first report of a production of this type of molecules by the genus Nocardiopsis. The new natural compound was established as (-)-7-deoxy-8-O-methyltetrangomycin with a new configuration.

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          Author and article information

          Journal
          World J. Microbiol. Biotechnol.
          World journal of microbiology & biotechnology
          Springer Nature
          1573-0972
          0959-3993
          Jun 2017
          : 33
          : 6
          Affiliations
          [1 ] Equipe Microbiologie, Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologique-Université des Sciences et de la Technologie Houari Boumediene, BP 32, El Alia, 16111, Bab Ezzouar Alger, Algeria.
          [2 ] Equipe Environnement et Microbiologie, Institut Pluridisciplinaire de Recherche sur l'Environnement et les Matériaux, UMR 5254, Centre national de la recherche scientifique-Université de Pau et des Pays de l'Adour, Pau, France.
          [3 ] CNRS - Institut de Chimie des Substances Naturelles - Equipe Métabolites de Microorganismes Symbiotiques-Bioinspiration, 1 Avenue de la Terrasse, 91198, Gif-sur-Yvette, France.
          [4 ] Equipe Microbiologie, Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologique-Université des Sciences et de la Technologie Houari Boumediene, BP 32, El Alia, 16111, Bab Ezzouar Alger, Algeria. h_hacene@yahoo.fr.
          Article
          10.1007/s11274-017-2292-8
          10.1007/s11274-017-2292-8
          28547727
          9d79d78e-fadb-40ac-b531-f29295210932
          History

          Angucyclinone,Antimicrobial activity,Halotolerant,Nocardiopsis,Taxonomy

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