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      Erratum: Tingting Lian, et al. Identification of Site-Specific Stroke Biomarker Candidates by Laser Capture Microdissection and Labeled Reference Peptide. Int. J. Mol. Sci. 2015, 16, 13427–13441

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          Abstract

          The authors wish to make a change to their published paper [1]. Since 2013, the authors have performed multiple experiments in many animals, including mice and rats, at both the Sun Yat-Sen University (Guangzhou, China) and China Medical University (Shenyang, China). After carefully checking the records, the authors found that experiments of these three particular animals in this study were performed in the animal facilities of the China Medical University. Section 3.2. Animals should therefore read: “The protocol in the study was approved by the Institutional Animal Care and Use Committee of the China Medical University [SYXK(Liao)20150003]. All efforts were made to ensure the animals' welfare and to lessen the number of animals used. Three young adult male Sprague Dawley rats weighing 250–300 g were allowed free access to food and water before all procedures”. The change does not affect the scientific results. The manuscript will be updated, and the original will remain online on the article webpage.

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          Identification of Site-Specific Stroke Biomarker Candidates by Laser Capture Microdissection and Labeled Reference Peptide

          The search to date for accurate protein biomarkers in acute ischemic stroke has taken into consideration the stage and/or the size of infarction, but has not accounted for the site of stroke. In the present study, multiple reaction monitoring using labeled reference peptide (LRP) following laser capture microdissection (LCM) is used to identify site-specific protein biomarker candidates. In middle cerebral artery occlusion (MCAO) rat models, both intact and infarcted brain tissue was collected by LCM, followed by on-film digestion and semi-quantification using triple-quadrupole mass spectrometry. Thirty-four unique peptides were detected for the verification of 12 proteins in both tissue homogenates and LCM-captured samples. Six insoluble proteins, including neurofilament light polypeptide (NEFL), alpha-internexin (INA), microtubule-associated protein 2 (MAP2), myelin basic protein (MBP), myelin proteolipid protein (PLP) and 2′,3′-cyclic-nucleotide 3′-phosphodiesterase (CNP), were found to be site-specific. Soluble proteins, such as neuron-specific enolase (NSE) and ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), and some insoluble proteins, including neurofilament heavy polypeptide (NEFH), glial fibrillary acidic protein (GFAP), microtubule-associated protein tau (MAPT) and tubulin β-3 chain (TUBB3), were found to be evenly distributed in the brain. Therefore, we conclude that some insoluble protein biomarkers for stroke are site-specific, and would make excellent candidates for the design and analysis of relevant clinical studies in the future.
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            Author and article information

            Journal
            Int J Mol Sci
            Int J Mol Sci
            ijms
            International Journal of Molecular Sciences
            MDPI
            1422-0067
            21 April 2017
            April 2017
            : 18
            : 4
            : 881
            Affiliations
            School of Bioscience and Bioengineering, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, China; l.tt04@ 123456mail.scut.edu.cn (T.L.); qu.daixin@ 123456mail.scut.edu.cn (D.Q.); rogerxu8911@ 123456gmail.com (X.Z.); yulixia2015@ 123456gmail.com (L.Y.)
            Author notes
            [* ]Correspondence: binggao@ 123456scut.edu.cn ; Tel./Fax: +86-20-3938-0618
            [†]

            These authors contributed equally to this work.

            Article
            ijms-18-00881
            10.3390/ijms18040881
            5412462
            28430125
            9d9495e9-dd50-45af-945d-575fa53d0686
            © 2017 by the authors.

            Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

            History
            : 19 April 2017
            : 20 April 2017
            Categories
            Erratum

            Molecular biology
            Molecular biology

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