3
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Estudio comparativo de las técnicas utilizadas para el diagnóstico de bacteriemia en infecciones relacionadas a catéteres

      research-article

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Resumen Las infecciones relacionadas con catéteres representan una significativa fuente de morbilidad, mortalidad y prolongación de la estancia hospitalaria. El diagnóstico bacteriológico puede abordarse mediante diversas metodologías. Este estudio tuvo como objetivo evaluar la sensibilidad y especificidad de las técnicas comúnmente empleadas en el laboratorio de microbiología. Se analizaron 180 catéteres por medio de las técnicas de Maki, sonicación (técnica de Sherertz) y vórtex (técnica de Brun-Buisson). Cada técnica fue aplicada en primer, segundo y tercer lugar, en un orden sistemático. La sensibilidad y especificidad del método de Maki fueron del 67% y 75% respectivamente, mientras que para las técnicas cuantitativas de Brun-Buisson y Sherertz fueron del 78% y 83%, y del 56% y 88% respectivamente. Se observó que la técnica de Brun-Buisson funcionó mejor cuando se aplicó en primer lugar, la de Sherertz en segundo lugar y la de Maki en tercer lugar.

          Translated abstract

          Abstract Catheter-related infections represent a significant source of morbidity, mortality and prolongation of hospital stay. Bacteriological diagnosis can be approached using various methodologies. This study aimed at evaluating the sensitivity and specificity of the techniques commonly used in the microbiology laboratory. One hundred and eighty catheters were analysed using the Maki, sonication (Sherertz technique) and vortex (Brun-Buisson technique) techniques. Each technique was applied first, second and third, in a systematic order. The sensitivity and specificity of the Maki method were 67% and 75% respectively, while for the Brun-Buisson and Sherertz quantitative techniques they were 78% and 83%, and 56% and 88% respectively. It was observed that the Brun-Buisson technique performed best when applied first, the Sherertz technique second, and the Maki technique third.

          Translated abstract

          Resumo As infecções relacionadas ao cateter representam uma fonte significativa de morbidade, mortalidade e prolongamento da internação hospitalar. O diagnóstico bacteriológico pode ser abordado por meio de diversas metodologias. Este estudo teve como objetivo avaliar a sensibilidade e especificidade das técnicas comumente utilizadas no laboratório de microbiologia. Foram analisados 180 cateteres pelas técnicas de Maki, sonicação (técnica de Sherertz) e vórtice (técnica de Brun-Buisson). Cada técnica foi aplicada em primeiro, segundo e terceiro lugar, em ordem sistemática. A sensibilidade e especificidade do método Maki foram de 67% e 75% respectivamente, enquanto que para as técnicas quantitativas de Brun-Buisson e Sherertz foram de 78% e 83%, e de 56% e 88% respectivamente. Observou-se que a técnica de Brun-Buisson teve melhor desempenho quando aplicada em primeiro lugar, a de Sherertz em segundo e a de Maki em terceiro.

          Related collections

          Most cited references15

          • Record: found
          • Abstract: found
          • Article: not found

          The risk of bloodstream infection in adults with different intravascular devices: a systematic review of 200 published prospective studies.

          To better understand the absolute and relative risks of bloodstream Infection (BSI) associated with the various types of intravascular devices (IVDs), we analyzed 200 published studies of adults In which every device in the study population was prospectively evaluated for evidence of associated infection and microbiologically based criteria were used to define IVD-related BSI. English-language reports of prospective studies of adults published between January 1, 1966, and July 1, 2005, were identified by MEDLINE search using the following general search strategy: bacteremla [Medical Subject Heading, MeSH] OR septicemia [MeSH] OR bloodstream Infection AND the specific type of intravascular device (e.g., central venous port). Mean rates of IVD-related BSI were calculated from pooled data for each type of device and expressed as BSIs per 100 IVDs (%) and per 1000 IVD days. Point incidence rates of IVD-related BSI were lowest with peripheral Intravenous catheters (0.1%, 0.5 per 1000 IVD-days) and midline catheters (0.4%, 0.2 per 1000 catheter-days). Far higher rates were seen with short-term noncuffed and nonmedicated central venous catheters (CVCs) (4.4%, 2.7 per 1000 catheter-days). Arterial catheters used for hemodynamic monitoring (0.8%, 1.7 per 1000 catheter-days) and peripherally inserted central catheters used in hospitalized patients (2.4%, 2.1 per 1000 catheter-days) posed risks approaching those seen with short-term conventional CVCs used in the Intensive care unit. Surgically implanted long-term central venous devices--cuffed and tunneled catheters (22.5%, 1.6 per 1000 IVD-days) and central venous ports (3.6%, 0.1 per 1000 IVD-days)--appear to have high rates of Infection when risk Is expressed as BSIs per 100 IVDs but actually pose much lower risk when rates are expressed per 1000 IVD-days. The use of cuffed and tunneled dual lumen CVCs rather than noncuffed, nontunneled catheters for temporary hemodlalysis and novel preventive technologies, such as CVCs with anti-infective surfaces, was associated with considerably lower rates of catheter-related BSI. Expressing risk of IVD-related BSI per 1000 IVD-days rather than BSIs per 100 IVDs allows for more meaningful estimates of risk. These data, based on prospective studies In which every IVD in the study cohort was analyzed for evidence of infection by microbiologically based criteria, show that all types of IVDs pose a risk of IVD-related BSI and can be used for benchmarking rates of infection caused by the various types of IVDs In use at the present time. Since almost all the national effort and progress to date to reduce the risk of IVD-related Infection have focused on short-term noncuffed CVCs used in Intensive care units, Infection control programs must now strive to consistently apply essential control measures and preventive technologies with all types of IVDs.
            • Record: found
            • Abstract: found
            • Article: not found

            A semiquantitative culture method for identifying intravenous-catheter-related infection.

            We evaluated a semiquantitative culture technic for identifying infection due to intravenous catheters: rolling the catheter segment across blood agar. This method was compared to broth culture. Of 250 catheters studied, 225 (90%) had low-density colonization on semiquantitative culture (less than 15 colonies on the plate) although 49 (19.6%) of these grew some organisms in broth or on the plate. None of these catheters led to septicemia. Twenty-five catheters (10%) grew greater than or equal to 15 colonies by the semiquantitative technic; most gave confluent growth. Septicemia originated from four of these catheters (P = 0.008). Of 37 catheters exposed to bacteremias from distant foci of infection, four yielded matching growth in broth, whereas none were concordant with the blood isolate on semiquantitative culture. Local inflammation was associated with high-density colonization semiquantitative culture (P less than 0.001). The semiquantitative technic distinguishes infection (greater than or equal to 15 colonies) from contamination and is more specific in diagnosis of catheter-related septicemia than culture of the catheter in broth.
              • Record: found
              • Abstract: found
              • Article: not found

              Diagnosis of central venous catheter-related sepsis. Critical level of quantitative tip cultures.

              The results of a simplified quantitative broth dilution quantitative tip culture (QTC) of 331 central venous catheters were compared with clinical data prospectively recorded in critically III patients to diagnose bacteremic or nonbacteremic catheter-related sepsis (CRS) (36 catheters), as opposed to contamination (42 catheters) or simple colonization from a distant septic focus (seven catheters). Thirty-five of 36 catheters associated with CRS yielded 10(3) colony-forming units per milliliter (CFU/mL) or more, and 3.8 X 10(2) Candida organisms grew from one. In contrast, 5 X 10(2) CFU/mL or less grew from 37 of 42 contaminated catheters. A QTC of 10(3) CFU/mL or more was 97.5% sensitive and 88% specific for the diagnosis of CRS. The QTC appeared especially useful for the diagnosis of CRS secondary to blood-borne seeding of catheters, or associated with coagulase-negative staphylococci.

                Author and article information

                Journal
                abcl
                Acta bioquímica clínica latinoamericana
                Acta bioquím. clín. latinoam.
                Federación Bioquímica de la Provincia de Buenos Aires (La Plata, Buenos Aires, Argentina )
                0325-2957
                1851-6114
                December 2024
                : 58
                : 4
                : 333-339
                Affiliations
                [1] orgnameServicio de Bacteriología Clínica, Departamento de Bacteriología. Instituto Nacional de Enfermedades Infecciosas INEI-ANLIS ‘‘Dr. Carlos G. Malbrán”, Ciudad Autónoma de Buenos Aires Argentina
                [2] orgnameSanatorio Sagrado Corazón de Alta Complejidad, Laboratorio de Bacteriología, Ciudad Autónoma de Buenos Aires Argentina
                [3] orgnameCátedra de Microbiología, Medicina, Universidad del Salvador, Ciudad Autónoma de Buenos Aires Argentina
                Author information
                https://orcid.org/0000-0001-5442-0586
                https://orcid.org/0000-0003-2518-8727
                Article
                S0325-29572024000400333 S0325-2957(24)05800400333
                9db1f455-5caf-40de-9eb7-cbc538f61992

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 13 May 2024
                : 11 June 2024
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 15, Pages: 7
                Product

                SciELO Argentina

                Categories
                Microbiología

                Diagnóstico microbiológico,Hemocultivos,Bacteriemas relacionadas a catéteres,Blood cultures,Catheter-related bacteremia,Microbiological diagnosis,Hemoculturas,Bacteremias relacionadas a cateteres

                Comments

                Comment on this article

                Related Documents Log